Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33855 and RR42_RS33860 are separated by 407 nucleotides RR42_RS33860 and RR42_RS33865 are separated by 140 nucleotides RR42_RS33865 and RR42_RS33870 are separated by 132 nucleotides
RR42_RS33855: RR42_RS33855 - TetR family transcriptional regulator, at 3,025,240 to 3,025,908
_RS33855
RR42_RS33860: RR42_RS33860 - 3-demethylubiquinone-9 3-methyltransferase, at 3,026,316 to 3,026,747
_RS33860
RR42_RS33865: RR42_RS33865 - hypothetical protein, at 3,026,888 to 3,027,088
_RS33865
RR42_RS33870: RR42_RS33870 - hypothetical protein, at 3,027,221 to 3,027,571
_RS33870
Position (kb)
3026
3027 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3025.392 kb on - strand, within RR42_RS33855 at 3025.392 kb on - strand, within RR42_RS33855 at 3025.470 kb on - strand, within RR42_RS33855 at 3025.583 kb on + strand, within RR42_RS33855 at 3025.583 kb on + strand, within RR42_RS33855 at 3025.868 kb on + strand at 3025.906 kb on + strand at 3025.906 kb on + strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3025.907 kb on - strand at 3026.049 kb on + strand at 3026.050 kb on - strand at 3026.055 kb on - strand at 3026.067 kb on - strand at 3026.320 kb on + strand at 3026.321 kb on - strand at 3026.331 kb on + strand at 3026.331 kb on + strand at 3026.331 kb on + strand at 3026.373 kb on + strand, within RR42_RS33860 at 3026.374 kb on - strand, within RR42_RS33860 at 3026.676 kb on + strand, within RR42_RS33860 at 3026.676 kb on + strand, within RR42_RS33860 at 3027.144 kb on + strand at 3027.144 kb on + strand at 3027.144 kb on + strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.145 kb on - strand at 3027.152 kb on + strand at 3027.153 kb on - strand at 3027.207 kb on + strand at 3027.208 kb on - strand at 3027.217 kb on + strand at 3027.217 kb on + strand at 3027.218 kb on - strand at 3027.218 kb on - strand at 3027.219 kb on + strand at 3027.220 kb on - strand at 3027.220 kb on - strand at 3027.453 kb on + strand, within RR42_RS33870 at 3027.453 kb on + strand, within RR42_RS33870 at 3027.454 kb on - strand, within RR42_RS33870 at 3027.476 kb on - strand, within RR42_RS33870 at 3027.476 kb on - strand, within RR42_RS33870 at 3027.565 kb on + strand at 3027.582 kb on + strand at 3027.582 kb on + strand at 3027.583 kb on - strand at 3027.583 kb on - strand at 3027.724 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.734 kb on + strand at 3027.735 kb on - strand at 3027.739 kb on + strand at 3027.739 kb on + strand at 3027.739 kb on + strand at 3027.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,025,392 - RR42_RS33855 0.23 +0.3 3,025,392 - RR42_RS33855 0.23 -0.2 3,025,470 - RR42_RS33855 0.34 +1.1 3,025,583 + RR42_RS33855 0.51 +1.4 3,025,583 + RR42_RS33855 0.51 -0.5 3,025,868 + +0.6 3,025,906 + -0.0 3,025,906 + -0.7 3,025,907 - -0.5 3,025,907 - -0.6 3,025,907 - -1.4 3,025,907 - +1.2 3,025,907 - +0.8 3,026,049 + -0.9 3,026,050 - -0.4 3,026,055 - +0.0 3,026,067 - +0.4 3,026,320 + +1.3 3,026,321 - -0.5 3,026,331 + +1.4 3,026,331 + +1.4 3,026,331 + +0.2 3,026,373 + RR42_RS33860 0.13 -0.2 3,026,374 - RR42_RS33860 0.13 -1.5 3,026,676 + RR42_RS33860 0.83 -3.6 3,026,676 + RR42_RS33860 0.83 -1.7 3,027,144 + +0.2 3,027,144 + -1.5 3,027,144 + +0.9 3,027,145 - -0.3 3,027,145 - +0.1 3,027,145 - +0.9 3,027,145 - -0.4 3,027,152 + +0.9 3,027,153 - -0.9 3,027,207 + +0.2 3,027,208 - -0.1 3,027,217 + +0.2 3,027,217 + +1.2 3,027,218 - -0.3 3,027,218 - +0.6 3,027,219 + -0.5 3,027,220 - -0.4 3,027,220 - -1.5 3,027,453 + RR42_RS33870 0.66 +0.7 3,027,453 + RR42_RS33870 0.66 +1.0 3,027,454 - RR42_RS33870 0.66 +0.1 3,027,476 - RR42_RS33870 0.73 +0.2 3,027,476 - RR42_RS33870 0.73 -0.1 3,027,565 + -0.0 3,027,582 + +0.3 3,027,582 + -0.1 3,027,583 - +0.8 3,027,583 - +0.8 3,027,724 + +0.2 3,027,734 + -0.7 3,027,734 + +0.3 3,027,734 + +0.9 3,027,734 + -0.6 3,027,734 + -1.1 3,027,735 - +0.6 3,027,739 + -0.9 3,027,739 + -0.9 3,027,739 + -0.5 3,027,740 - +0.4
Or see this region's nucleotide sequence