Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33550 and RR42_RS33555 are separated by 35 nucleotides RR42_RS33555 and RR42_RS33560 are separated by 84 nucleotides RR42_RS33560 and RR42_RS33565 are separated by 76 nucleotides
RR42_RS33550: RR42_RS33550 - secretion protein HylD, at 2,943,576 to 2,944,880
_RS33550
RR42_RS33555: RR42_RS33555 - secretion protein HlyD, at 2,944,916 to 2,945,686
_RS33555
RR42_RS33560: RR42_RS33560 - transporter, at 2,945,771 to 2,947,279
_RS33560
RR42_RS33565: RR42_RS33565 - hypothetical protein, at 2,947,356 to 2,954,855
_RS33565
Position (kb)
2945
2946
2947
2948 Strain fitness (log2 ratio)
-2
-1
0
1 at 2944.780 kb on + strand at 2945.275 kb on + strand, within RR42_RS33555 at 2945.275 kb on + strand, within RR42_RS33555 at 2945.276 kb on - strand, within RR42_RS33555 at 2945.276 kb on - strand, within RR42_RS33555 at 2945.276 kb on - strand, within RR42_RS33555 at 2945.276 kb on - strand, within RR42_RS33555 at 2945.276 kb on - strand, within RR42_RS33555 at 2945.277 kb on + strand, within RR42_RS33555 at 2945.277 kb on + strand, within RR42_RS33555 at 2945.278 kb on - strand, within RR42_RS33555 at 2945.278 kb on - strand, within RR42_RS33555 at 2945.711 kb on + strand at 2945.772 kb on + strand at 2945.773 kb on - strand at 2945.773 kb on - strand at 2945.773 kb on - strand at 2945.773 kb on - strand at 2945.848 kb on - strand at 2945.935 kb on - strand, within RR42_RS33560 at 2946.023 kb on - strand, within RR42_RS33560 at 2946.084 kb on + strand, within RR42_RS33560 at 2946.093 kb on + strand, within RR42_RS33560 at 2946.093 kb on + strand, within RR42_RS33560 at 2946.093 kb on + strand, within RR42_RS33560 at 2946.094 kb on - strand, within RR42_RS33560 at 2946.094 kb on - strand, within RR42_RS33560 at 2946.094 kb on - strand, within RR42_RS33560 at 2946.103 kb on - strand, within RR42_RS33560 at 2946.168 kb on + strand, within RR42_RS33560 at 2946.168 kb on + strand, within RR42_RS33560 at 2946.369 kb on + strand, within RR42_RS33560 at 2946.437 kb on + strand, within RR42_RS33560 at 2946.477 kb on + strand, within RR42_RS33560 at 2946.477 kb on + strand, within RR42_RS33560 at 2946.478 kb on - strand, within RR42_RS33560 at 2946.588 kb on + strand, within RR42_RS33560 at 2946.589 kb on - strand, within RR42_RS33560 at 2946.589 kb on - strand, within RR42_RS33560 at 2946.720 kb on + strand, within RR42_RS33560 at 2946.813 kb on + strand, within RR42_RS33560 at 2946.813 kb on + strand, within RR42_RS33560 at 2946.813 kb on + strand, within RR42_RS33560 at 2946.814 kb on - strand, within RR42_RS33560 at 2946.817 kb on - strand, within RR42_RS33560 at 2946.817 kb on - strand, within RR42_RS33560 at 2946.865 kb on + strand, within RR42_RS33560 at 2946.866 kb on - strand, within RR42_RS33560 at 2946.866 kb on - strand, within RR42_RS33560 at 2946.867 kb on + strand, within RR42_RS33560 at 2946.867 kb on + strand, within RR42_RS33560 at 2946.868 kb on - strand, within RR42_RS33560 at 2946.868 kb on - strand, within RR42_RS33560 at 2947.068 kb on + strand, within RR42_RS33560 at 2947.068 kb on + strand, within RR42_RS33560 at 2947.069 kb on - strand, within RR42_RS33560 at 2947.103 kb on - strand, within RR42_RS33560 at 2947.279 kb on + strand at 2947.280 kb on - strand at 2947.324 kb on + strand at 2947.324 kb on + strand at 2947.326 kb on + strand at 2947.327 kb on - strand at 2947.327 kb on - strand at 2947.357 kb on + strand at 2947.630 kb on - strand at 2947.978 kb on + strand at 2947.978 kb on + strand at 2947.978 kb on + strand at 2947.979 kb on - strand at 2947.979 kb on - strand at 2947.979 kb on - strand at 2948.221 kb on + strand, within RR42_RS33565 at 2948.222 kb on - strand, within RR42_RS33565
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,944,780 + +1.6 2,945,275 + RR42_RS33555 0.47 -0.8 2,945,275 + RR42_RS33555 0.47 -0.5 2,945,276 - RR42_RS33555 0.47 -0.4 2,945,276 - RR42_RS33555 0.47 +0.0 2,945,276 - RR42_RS33555 0.47 +0.3 2,945,276 - RR42_RS33555 0.47 -0.5 2,945,276 - RR42_RS33555 0.47 +0.2 2,945,277 + RR42_RS33555 0.47 +0.1 2,945,277 + RR42_RS33555 0.47 +0.4 2,945,278 - RR42_RS33555 0.47 +1.1 2,945,278 - RR42_RS33555 0.47 +0.8 2,945,711 + -2.7 2,945,772 + -0.2 2,945,773 - -0.3 2,945,773 - +0.3 2,945,773 - -0.3 2,945,773 - +0.2 2,945,848 - +0.8 2,945,935 - RR42_RS33560 0.11 -0.5 2,946,023 - RR42_RS33560 0.17 -1.8 2,946,084 + RR42_RS33560 0.21 -1.3 2,946,093 + RR42_RS33560 0.21 +0.5 2,946,093 + RR42_RS33560 0.21 +0.3 2,946,093 + RR42_RS33560 0.21 -0.4 2,946,094 - RR42_RS33560 0.21 +0.4 2,946,094 - RR42_RS33560 0.21 -1.0 2,946,094 - RR42_RS33560 0.21 +0.6 2,946,103 - RR42_RS33560 0.22 +1.2 2,946,168 + RR42_RS33560 0.26 -0.4 2,946,168 + RR42_RS33560 0.26 -0.3 2,946,369 + RR42_RS33560 0.40 +0.4 2,946,437 + RR42_RS33560 0.44 -0.6 2,946,477 + RR42_RS33560 0.47 +0.5 2,946,477 + RR42_RS33560 0.47 +0.2 2,946,478 - RR42_RS33560 0.47 -0.9 2,946,588 + RR42_RS33560 0.54 -0.2 2,946,589 - RR42_RS33560 0.54 +0.2 2,946,589 - RR42_RS33560 0.54 +0.6 2,946,720 + RR42_RS33560 0.63 +0.7 2,946,813 + RR42_RS33560 0.69 +1.0 2,946,813 + RR42_RS33560 0.69 -0.6 2,946,813 + RR42_RS33560 0.69 +0.4 2,946,814 - RR42_RS33560 0.69 -0.0 2,946,817 - RR42_RS33560 0.69 -0.3 2,946,817 - RR42_RS33560 0.69 -0.4 2,946,865 + RR42_RS33560 0.72 -0.4 2,946,866 - RR42_RS33560 0.73 -0.3 2,946,866 - RR42_RS33560 0.73 -0.6 2,946,867 + RR42_RS33560 0.73 -0.5 2,946,867 + RR42_RS33560 0.73 -0.1 2,946,868 - RR42_RS33560 0.73 -0.3 2,946,868 - RR42_RS33560 0.73 +0.0 2,947,068 + RR42_RS33560 0.86 -0.1 2,947,068 + RR42_RS33560 0.86 +0.1 2,947,069 - RR42_RS33560 0.86 +0.3 2,947,103 - RR42_RS33560 0.88 +0.1 2,947,279 + -0.4 2,947,280 - +0.6 2,947,324 + +0.6 2,947,324 + +0.3 2,947,326 + -0.2 2,947,327 - +0.1 2,947,327 - -0.2 2,947,357 + +1.3 2,947,630 - +0.1 2,947,978 + -0.4 2,947,978 + -1.0 2,947,978 + -0.4 2,947,979 - -0.1 2,947,979 - +0.3 2,947,979 - +1.4 2,948,221 + RR42_RS33565 0.12 +0.0 2,948,222 - RR42_RS33565 0.12 -0.0
Or see this region's nucleotide sequence