Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS33460 and RR42_RS33465 overlap by 4 nucleotides RR42_RS33465 and RR42_RS33470 are separated by 75 nucleotides RR42_RS33470 and RR42_RS33475 are separated by 78 nucleotides RR42_RS33475 and RR42_RS33480 are separated by 334 nucleotides
RR42_RS33460: RR42_RS33460 - glutathione S-transferase, at 2,924,848 to 2,925,933
_RS33460
RR42_RS33465: RR42_RS33465 - ArsR family transcriptional regulator, at 2,925,930 to 2,926,313
_RS33465
RR42_RS33470: RR42_RS33470 - hypothetical protein, at 2,926,389 to 2,926,715
_RS33470
RR42_RS33475: RR42_RS33475 - CopG family transcriptional regulator, at 2,926,794 to 2,926,997
_RS33475
RR42_RS33480: RR42_RS33480 - spermidine synthase, at 2,927,332 to 2,928,147
_RS33480
Position (kb)
2926
2927 Strain fitness (log2 ratio)
-2
-1
0
1 at 2925.918 kb on + strand at 2925.918 kb on + strand at 2925.919 kb on - strand at 2925.919 kb on - strand at 2925.919 kb on - strand at 2925.919 kb on - strand at 2926.391 kb on - strand at 2926.407 kb on + strand at 2926.407 kb on + strand at 2926.407 kb on + strand at 2926.408 kb on - strand at 2926.408 kb on - strand at 2926.434 kb on - strand, within RR42_RS33470 at 2926.585 kb on + strand, within RR42_RS33470 at 2926.586 kb on - strand, within RR42_RS33470 at 2926.586 kb on - strand, within RR42_RS33470 at 2926.586 kb on - strand, within RR42_RS33470 at 2926.621 kb on + strand, within RR42_RS33470 at 2926.622 kb on - strand, within RR42_RS33470 at 2926.622 kb on - strand, within RR42_RS33470 at 2926.625 kb on + strand, within RR42_RS33470 at 2926.625 kb on + strand, within RR42_RS33470 at 2926.648 kb on + strand, within RR42_RS33470 at 2926.648 kb on + strand, within RR42_RS33470 at 2926.648 kb on + strand, within RR42_RS33470 at 2926.649 kb on - strand, within RR42_RS33470 at 2926.649 kb on - strand, within RR42_RS33470 at 2926.649 kb on - strand, within RR42_RS33470 at 2926.649 kb on - strand, within RR42_RS33470 at 2926.649 kb on - strand, within RR42_RS33470 at 2926.652 kb on + strand, within RR42_RS33470 at 2926.652 kb on + strand, within RR42_RS33470 at 2926.653 kb on - strand, within RR42_RS33470 at 2926.690 kb on + strand at 2926.690 kb on + strand at 2926.767 kb on + strand at 2926.768 kb on - strand at 2926.768 kb on - strand at 2926.849 kb on + strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.850 kb on - strand, within RR42_RS33475 at 2926.873 kb on + strand, within RR42_RS33475 at 2927.013 kb on - strand at 2927.042 kb on - strand at 2927.134 kb on + strand at 2927.134 kb on + strand at 2927.135 kb on - strand at 2927.135 kb on - strand at 2927.135 kb on - strand at 2927.138 kb on + strand at 2927.146 kb on - strand at 2927.146 kb on - strand at 2927.146 kb on - strand at 2927.346 kb on + strand at 2927.346 kb on + strand at 2927.362 kb on + strand at 2927.363 kb on - strand at 2927.503 kb on + strand, within RR42_RS33480 at 2927.593 kb on + strand, within RR42_RS33480 at 2927.593 kb on + strand, within RR42_RS33480 at 2927.593 kb on + strand, within RR42_RS33480 at 2927.593 kb on + strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.594 kb on - strand, within RR42_RS33480 at 2927.729 kb on - strand, within RR42_RS33480 at 2927.729 kb on - strand, within RR42_RS33480 at 2927.729 kb on - strand, within RR42_RS33480 at 2927.729 kb on - strand, within RR42_RS33480 at 2927.821 kb on + strand, within RR42_RS33480 at 2927.858 kb on + strand, within RR42_RS33480 at 2927.858 kb on + strand, within RR42_RS33480 at 2927.859 kb on - strand, within RR42_RS33480 at 2927.872 kb on + strand, within RR42_RS33480 at 2927.872 kb on + strand, within RR42_RS33480 at 2927.873 kb on - strand, within RR42_RS33480 at 2927.892 kb on + strand, within RR42_RS33480 at 2927.902 kb on + strand, within RR42_RS33480 at 2927.903 kb on - strand, within RR42_RS33480 at 2927.971 kb on + strand, within RR42_RS33480 at 2927.971 kb on + strand, within RR42_RS33480 at 2927.972 kb on - strand, within RR42_RS33480 at 2927.972 kb on - strand, within RR42_RS33480
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,925,918 + +0.0 2,925,918 + +0.7 2,925,919 - -0.6 2,925,919 - +0.3 2,925,919 - -0.1 2,925,919 - -0.2 2,926,391 - -0.4 2,926,407 + +0.3 2,926,407 + -0.4 2,926,407 + -0.1 2,926,408 - +0.7 2,926,408 - -1.3 2,926,434 - RR42_RS33470 0.14 +0.1 2,926,585 + RR42_RS33470 0.60 +0.7 2,926,586 - RR42_RS33470 0.60 +0.4 2,926,586 - RR42_RS33470 0.60 -0.6 2,926,586 - RR42_RS33470 0.60 +1.3 2,926,621 + RR42_RS33470 0.71 -0.9 2,926,622 - RR42_RS33470 0.71 +0.3 2,926,622 - RR42_RS33470 0.71 +0.1 2,926,625 + RR42_RS33470 0.72 -0.7 2,926,625 + RR42_RS33470 0.72 +0.9 2,926,648 + RR42_RS33470 0.79 -0.4 2,926,648 + RR42_RS33470 0.79 -1.2 2,926,648 + RR42_RS33470 0.79 +0.1 2,926,649 - RR42_RS33470 0.80 -0.7 2,926,649 - RR42_RS33470 0.80 -0.6 2,926,649 - RR42_RS33470 0.80 +0.4 2,926,649 - RR42_RS33470 0.80 +0.4 2,926,649 - RR42_RS33470 0.80 +0.0 2,926,652 + RR42_RS33470 0.80 -1.4 2,926,652 + RR42_RS33470 0.80 -0.3 2,926,653 - RR42_RS33470 0.81 +0.6 2,926,690 + -0.1 2,926,690 + -0.5 2,926,767 + +0.0 2,926,768 - +0.0 2,926,768 - -0.0 2,926,849 + RR42_RS33475 0.27 -1.0 2,926,850 - RR42_RS33475 0.27 -0.5 2,926,850 - RR42_RS33475 0.27 +0.2 2,926,850 - RR42_RS33475 0.27 -1.0 2,926,850 - RR42_RS33475 0.27 -0.7 2,926,850 - RR42_RS33475 0.27 -1.2 2,926,850 - RR42_RS33475 0.27 -2.6 2,926,873 + RR42_RS33475 0.39 -0.4 2,927,013 - -0.4 2,927,042 - +0.4 2,927,134 + +0.3 2,927,134 + +0.2 2,927,135 - -0.2 2,927,135 - +0.1 2,927,135 - -0.1 2,927,138 + -1.0 2,927,146 - +0.4 2,927,146 - -0.1 2,927,146 - -0.3 2,927,346 + +0.8 2,927,346 + +0.2 2,927,362 + -0.0 2,927,363 - +0.2 2,927,503 + RR42_RS33480 0.21 -1.4 2,927,593 + RR42_RS33480 0.32 -2.0 2,927,593 + RR42_RS33480 0.32 +0.1 2,927,593 + RR42_RS33480 0.32 +0.1 2,927,593 + RR42_RS33480 0.32 -0.2 2,927,594 - RR42_RS33480 0.32 -1.9 2,927,594 - RR42_RS33480 0.32 +0.1 2,927,594 - RR42_RS33480 0.32 -0.2 2,927,594 - RR42_RS33480 0.32 +0.8 2,927,594 - RR42_RS33480 0.32 +0.1 2,927,594 - RR42_RS33480 0.32 +0.2 2,927,594 - RR42_RS33480 0.32 -0.2 2,927,594 - RR42_RS33480 0.32 -1.1 2,927,594 - RR42_RS33480 0.32 -1.3 2,927,729 - RR42_RS33480 0.49 -0.2 2,927,729 - RR42_RS33480 0.49 -0.3 2,927,729 - RR42_RS33480 0.49 -0.3 2,927,729 - RR42_RS33480 0.49 +0.0 2,927,821 + RR42_RS33480 0.60 -0.6 2,927,858 + RR42_RS33480 0.64 -0.6 2,927,858 + RR42_RS33480 0.64 +0.1 2,927,859 - RR42_RS33480 0.65 +0.4 2,927,872 + RR42_RS33480 0.66 +0.9 2,927,872 + RR42_RS33480 0.66 -0.9 2,927,873 - RR42_RS33480 0.66 +0.1 2,927,892 + RR42_RS33480 0.69 -2.1 2,927,902 + RR42_RS33480 0.70 +1.1 2,927,903 - RR42_RS33480 0.70 +0.0 2,927,971 + RR42_RS33480 0.78 +0.1 2,927,971 + RR42_RS33480 0.78 -0.2 2,927,972 - RR42_RS33480 0.78 +0.0 2,927,972 - RR42_RS33480 0.78 -1.4
Or see this region's nucleotide sequence