Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS33305

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS33300 and RR42_RS33305 are separated by 213 nucleotidesRR42_RS33305 and RR42_RS33310 are separated by 46 nucleotidesRR42_RS33310 and RR42_RS33315 are separated by 150 nucleotides RR42_RS33300: RR42_RS33300 - transcriptional regulator, at 2,886,204 to 2,886,905 _RS33300 RR42_RS33305: RR42_RS33305 - dihydroxy-acid dehydratase, at 2,887,119 to 2,888,858 _RS33305 RR42_RS33310: RR42_RS33310 - hypothetical protein, at 2,888,905 to 2,889,642 _RS33310 RR42_RS33315: RR42_RS33315 - ABC transporter substrate-binding protein, at 2,889,793 to 2,890,797 _RS33315 Position (kb) 2887 2888 2889Strain fitness (log2 ratio) -2 -1 0 1 2at 2886.194 kb on - strandat 2886.194 kb on - strandat 2886.206 kb on - strandat 2886.224 kb on - strandat 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.429 kb on + strand, within RR42_RS33300at 2886.430 kb on - strand, within RR42_RS33300at 2886.430 kb on - strand, within RR42_RS33300at 2886.430 kb on - strand, within RR42_RS33300at 2886.455 kb on - strand, within RR42_RS33300at 2886.455 kb on - strand, within RR42_RS33300at 2886.686 kb on + strand, within RR42_RS33300at 2886.687 kb on - strand, within RR42_RS33300at 2886.824 kb on + strand, within RR42_RS33300at 2886.824 kb on + strand, within RR42_RS33300at 2886.824 kb on + strand, within RR42_RS33300at 2886.940 kb on - strandat 2886.983 kb on - strandat 2887.225 kb on - strandat 2887.557 kb on + strand, within RR42_RS33305at 2887.557 kb on + strand, within RR42_RS33305at 2887.557 kb on + strand, within RR42_RS33305at 2887.557 kb on + strand, within RR42_RS33305at 2887.557 kb on + strand, within RR42_RS33305at 2887.558 kb on - strand, within RR42_RS33305at 2887.558 kb on - strand, within RR42_RS33305at 2887.558 kb on - strand, within RR42_RS33305at 2887.658 kb on + strand, within RR42_RS33305at 2888.073 kb on + strand, within RR42_RS33305at 2888.074 kb on - strand, within RR42_RS33305at 2888.074 kb on - strand, within RR42_RS33305at 2888.074 kb on - strand, within RR42_RS33305at 2888.074 kb on - strand, within RR42_RS33305at 2888.095 kb on - strand, within RR42_RS33305at 2888.095 kb on - strand, within RR42_RS33305at 2888.095 kb on - strand, within RR42_RS33305at 2888.205 kb on + strand, within RR42_RS33305at 2888.205 kb on + strand, within RR42_RS33305at 2888.205 kb on + strand, within RR42_RS33305at 2888.206 kb on - strand, within RR42_RS33305at 2888.206 kb on - strand, within RR42_RS33305at 2888.208 kb on - strand, within RR42_RS33305at 2888.244 kb on + strand, within RR42_RS33305at 2888.411 kb on + strand, within RR42_RS33305at 2888.566 kb on - strand, within RR42_RS33305at 2888.601 kb on + strand, within RR42_RS33305at 2888.601 kb on + strand, within RR42_RS33305at 2888.602 kb on - strand, within RR42_RS33305at 2888.603 kb on + strand, within RR42_RS33305at 2888.604 kb on - strand, within RR42_RS33305at 2888.853 kb on + strandat 2888.853 kb on + strandat 2888.853 kb on + strandat 2888.853 kb on + strandat 2888.853 kb on + strandat 2888.853 kb on + strandat 2888.854 kb on - strandat 2888.854 kb on - strandat 2888.854 kb on - strandat 2888.913 kb on + strandat 2888.913 kb on + strandat 2889.088 kb on + strand, within RR42_RS33310at 2889.088 kb on + strand, within RR42_RS33310at 2889.088 kb on + strand, within RR42_RS33310at 2889.089 kb on - strand, within RR42_RS33310at 2889.334 kb on + strand, within RR42_RS33310at 2889.334 kb on + strand, within RR42_RS33310at 2889.335 kb on - strand, within RR42_RS33310at 2889.335 kb on - strand, within RR42_RS33310at 2889.335 kb on - strand, within RR42_RS33310

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,886,194 - -0.4
2,886,194 - +0.2
2,886,206 - +0.1
2,886,224 - +2.2
2,886,429 + RR42_RS33300 0.32 +0.1
2,886,429 + RR42_RS33300 0.32 +0.2
2,886,429 + RR42_RS33300 0.32 -2.0
2,886,429 + RR42_RS33300 0.32 +0.1
2,886,429 + RR42_RS33300 0.32 +0.5
2,886,429 + RR42_RS33300 0.32 +0.4
2,886,429 + RR42_RS33300 0.32 -0.2
2,886,429 + RR42_RS33300 0.32 -0.4
2,886,429 + RR42_RS33300 0.32 -0.0
2,886,430 - RR42_RS33300 0.32 +1.1
2,886,430 - RR42_RS33300 0.32 +0.7
2,886,430 - RR42_RS33300 0.32 +0.2
2,886,455 - RR42_RS33300 0.36 +0.2
2,886,455 - RR42_RS33300 0.36 -0.3
2,886,686 + RR42_RS33300 0.69 -0.4
2,886,687 - RR42_RS33300 0.69 +0.2
2,886,824 + RR42_RS33300 0.88 -0.4
2,886,824 + RR42_RS33300 0.88 -0.4
2,886,824 + RR42_RS33300 0.88 +1.1
2,886,940 - +0.2
2,886,983 - -0.8
2,887,225 - -0.1
2,887,557 + RR42_RS33305 0.25 +0.1
2,887,557 + RR42_RS33305 0.25 -1.0
2,887,557 + RR42_RS33305 0.25 +0.9
2,887,557 + RR42_RS33305 0.25 +0.2
2,887,557 + RR42_RS33305 0.25 -1.2
2,887,558 - RR42_RS33305 0.25 +0.3
2,887,558 - RR42_RS33305 0.25 +1.2
2,887,558 - RR42_RS33305 0.25 +0.1
2,887,658 + RR42_RS33305 0.31 +0.7
2,888,073 + RR42_RS33305 0.55 -0.6
2,888,074 - RR42_RS33305 0.55 -0.7
2,888,074 - RR42_RS33305 0.55 +0.1
2,888,074 - RR42_RS33305 0.55 -0.4
2,888,074 - RR42_RS33305 0.55 +0.0
2,888,095 - RR42_RS33305 0.56 -0.9
2,888,095 - RR42_RS33305 0.56 +0.6
2,888,095 - RR42_RS33305 0.56 +0.4
2,888,205 + RR42_RS33305 0.62 +0.0
2,888,205 + RR42_RS33305 0.62 +0.9
2,888,205 + RR42_RS33305 0.62 -0.3
2,888,206 - RR42_RS33305 0.62 -0.5
2,888,206 - RR42_RS33305 0.62 -0.4
2,888,208 - RR42_RS33305 0.63 +0.9
2,888,244 + RR42_RS33305 0.65 -0.2
2,888,411 + RR42_RS33305 0.74 +0.1
2,888,566 - RR42_RS33305 0.83 -0.0
2,888,601 + RR42_RS33305 0.85 -0.3
2,888,601 + RR42_RS33305 0.85 +0.7
2,888,602 - RR42_RS33305 0.85 -0.8
2,888,603 + RR42_RS33305 0.85 -0.4
2,888,604 - RR42_RS33305 0.85 +0.1
2,888,853 + +0.1
2,888,853 + +0.5
2,888,853 + +0.3
2,888,853 + -2.7
2,888,853 + -0.6
2,888,853 + +0.0
2,888,854 - -0.3
2,888,854 - +0.2
2,888,854 - +0.4
2,888,913 + -0.0
2,888,913 + +0.8
2,889,088 + RR42_RS33310 0.25 -0.4
2,889,088 + RR42_RS33310 0.25 +0.5
2,889,088 + RR42_RS33310 0.25 -0.5
2,889,089 - RR42_RS33310 0.25 +0.0
2,889,334 + RR42_RS33310 0.58 +0.6
2,889,334 + RR42_RS33310 0.58 -2.0
2,889,335 - RR42_RS33310 0.58 -0.0
2,889,335 - RR42_RS33310 0.58 -1.1
2,889,335 - RR42_RS33310 0.58 -0.4

Or see this region's nucleotide sequence