Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31185 and RR42_RS31190 overlap by 4 nucleotides RR42_RS31190 and RR42_RS31195 overlap by 4 nucleotides RR42_RS31195 and RR42_RS31200 are separated by 2 nucleotides
RR42_RS31185: RR42_RS31185 - 3-oxoacyl-ACP synthase, at 2,374,286 to 2,375,512
_RS31185
RR42_RS31190: RR42_RS31190 - 3-ketoacyl-ACP reductase, at 2,375,509 to 2,376,240
_RS31190
RR42_RS31195: RR42_RS31195 - dehydratase, at 2,376,237 to 2,376,707
_RS31195
RR42_RS31200: RR42_RS31200 - 3-oxoacyl-ACP synthase, at 2,376,710 to 2,377,891
_RS31200
Position (kb)
2375
2376
2377 Strain fitness (log2 ratio)
-2
-1
0
1 at 2374.516 kb on + strand, within RR42_RS31185 at 2374.517 kb on - strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.518 kb on + strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.519 kb on - strand, within RR42_RS31185 at 2374.623 kb on + strand, within RR42_RS31185 at 2374.623 kb on + strand, within RR42_RS31185 at 2374.989 kb on + strand, within RR42_RS31185 at 2374.989 kb on + strand, within RR42_RS31185 at 2375.035 kb on + strand, within RR42_RS31185 at 2375.035 kb on + strand, within RR42_RS31185 at 2375.053 kb on + strand, within RR42_RS31185 at 2375.109 kb on + strand, within RR42_RS31185 at 2375.109 kb on + strand, within RR42_RS31185 at 2375.110 kb on - strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.144 kb on + strand, within RR42_RS31185 at 2375.145 kb on - strand, within RR42_RS31185 at 2375.199 kb on + strand, within RR42_RS31185 at 2375.200 kb on - strand, within RR42_RS31185 at 2375.328 kb on + strand, within RR42_RS31185 at 2375.328 kb on + strand, within RR42_RS31185 at 2375.329 kb on - strand, within RR42_RS31185 at 2375.329 kb on - strand, within RR42_RS31185 at 2375.553 kb on + strand at 2375.553 kb on + strand at 2375.554 kb on - strand at 2375.555 kb on + strand at 2375.556 kb on - strand at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.780 kb on + strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.781 kb on - strand, within RR42_RS31190 at 2375.891 kb on + strand, within RR42_RS31190 at 2375.892 kb on - strand, within RR42_RS31190 at 2375.994 kb on - strand, within RR42_RS31190 at 2375.997 kb on - strand, within RR42_RS31190 at 2376.159 kb on - strand, within RR42_RS31190 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.384 kb on + strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.385 kb on - strand, within RR42_RS31195 at 2376.431 kb on - strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.523 kb on + strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.524 kb on - strand, within RR42_RS31195 at 2376.725 kb on + strand at 2376.726 kb on - strand at 2376.726 kb on - strand at 2377.056 kb on + strand, within RR42_RS31200 at 2377.057 kb on - strand, within RR42_RS31200
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,374,516 + RR42_RS31185 0.19 -0.9 2,374,517 - RR42_RS31185 0.19 -0.5 2,374,518 + RR42_RS31185 0.19 +1.2 2,374,518 + RR42_RS31185 0.19 +0.2 2,374,518 + RR42_RS31185 0.19 +0.5 2,374,518 + RR42_RS31185 0.19 -1.4 2,374,519 - RR42_RS31185 0.19 -0.6 2,374,519 - RR42_RS31185 0.19 -0.3 2,374,519 - RR42_RS31185 0.19 -1.9 2,374,519 - RR42_RS31185 0.19 -0.9 2,374,519 - RR42_RS31185 0.19 +0.9 2,374,519 - RR42_RS31185 0.19 -0.3 2,374,519 - RR42_RS31185 0.19 +0.0 2,374,623 + RR42_RS31185 0.27 +0.6 2,374,623 + RR42_RS31185 0.27 -1.3 2,374,989 + RR42_RS31185 0.57 +0.1 2,374,989 + RR42_RS31185 0.57 +0.2 2,375,035 + RR42_RS31185 0.61 -0.2 2,375,035 + RR42_RS31185 0.61 -0.2 2,375,053 + RR42_RS31185 0.63 +0.2 2,375,109 + RR42_RS31185 0.67 +0.3 2,375,109 + RR42_RS31185 0.67 -0.2 2,375,110 - RR42_RS31185 0.67 -0.1 2,375,144 + RR42_RS31185 0.70 -0.9 2,375,144 + RR42_RS31185 0.70 +0.5 2,375,144 + RR42_RS31185 0.70 +0.1 2,375,144 + RR42_RS31185 0.70 +1.2 2,375,144 + RR42_RS31185 0.70 +0.2 2,375,145 - RR42_RS31185 0.70 -0.2 2,375,199 + RR42_RS31185 0.74 -0.2 2,375,200 - RR42_RS31185 0.74 -0.5 2,375,328 + RR42_RS31185 0.85 -0.1 2,375,328 + RR42_RS31185 0.85 +0.3 2,375,329 - RR42_RS31185 0.85 -0.8 2,375,329 - RR42_RS31185 0.85 +0.2 2,375,553 + -0.2 2,375,553 + -0.4 2,375,554 - -0.2 2,375,555 + -0.9 2,375,556 - +0.1 2,375,780 + RR42_RS31190 0.37 +0.6 2,375,780 + RR42_RS31190 0.37 +0.2 2,375,780 + RR42_RS31190 0.37 -0.7 2,375,780 + RR42_RS31190 0.37 -2.1 2,375,780 + RR42_RS31190 0.37 -1.3 2,375,781 - RR42_RS31190 0.37 +0.5 2,375,781 - RR42_RS31190 0.37 -0.2 2,375,781 - RR42_RS31190 0.37 +0.4 2,375,781 - RR42_RS31190 0.37 +0.0 2,375,891 + RR42_RS31190 0.52 -1.0 2,375,892 - RR42_RS31190 0.52 -0.2 2,375,994 - RR42_RS31190 0.66 -1.4 2,375,997 - RR42_RS31190 0.67 +0.6 2,376,159 - RR42_RS31190 0.89 +0.9 2,376,384 + RR42_RS31195 0.31 +0.1 2,376,384 + RR42_RS31195 0.31 -0.2 2,376,385 - RR42_RS31195 0.31 +0.3 2,376,385 - RR42_RS31195 0.31 +0.8 2,376,431 - RR42_RS31195 0.41 -0.5 2,376,523 + RR42_RS31195 0.61 -0.9 2,376,523 + RR42_RS31195 0.61 -1.9 2,376,524 - RR42_RS31195 0.61 +0.1 2,376,524 - RR42_RS31195 0.61 -0.3 2,376,725 + -1.0 2,376,726 - +0.4 2,376,726 - -1.4 2,377,056 + RR42_RS31200 0.29 +0.8 2,377,057 - RR42_RS31200 0.29 -0.6
Or see this region's nucleotide sequence