Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29860 and RR42_RS29865 are separated by 341 nucleotides RR42_RS29865 and RR42_RS29870 are separated by 411 nucleotides
RR42_RS29860: RR42_RS29860 - aldehyde dehydrogenase, at 2,064,370 to 2,065,797
_RS29860
RR42_RS29865: RR42_RS29865 - hypothetical protein, at 2,066,139 to 2,066,396
_RS29865
RR42_RS29870: RR42_RS29870 - anhydrase, at 2,066,808 to 2,067,332
_RS29870
Position (kb)
2066
2067 Strain fitness (log2 ratio)
-2
-1
0
1 at 2065.189 kb on + strand, within RR42_RS29860 at 2065.190 kb on - strand, within RR42_RS29860 at 2065.190 kb on - strand, within RR42_RS29860 at 2065.190 kb on - strand, within RR42_RS29860 at 2065.214 kb on + strand, within RR42_RS29860 at 2065.214 kb on + strand, within RR42_RS29860 at 2065.214 kb on + strand, within RR42_RS29860 at 2065.215 kb on - strand, within RR42_RS29860 at 2065.215 kb on - strand, within RR42_RS29860 at 2065.215 kb on - strand, within RR42_RS29860 at 2065.215 kb on - strand, within RR42_RS29860 at 2065.255 kb on + strand, within RR42_RS29860 at 2065.255 kb on + strand, within RR42_RS29860 at 2065.255 kb on + strand, within RR42_RS29860 at 2065.260 kb on + strand, within RR42_RS29860 at 2065.261 kb on - strand, within RR42_RS29860 at 2065.261 kb on - strand, within RR42_RS29860 at 2065.279 kb on - strand, within RR42_RS29860 at 2065.316 kb on + strand, within RR42_RS29860 at 2065.316 kb on + strand, within RR42_RS29860 at 2065.316 kb on + strand, within RR42_RS29860 at 2065.316 kb on + strand, within RR42_RS29860 at 2065.317 kb on - strand, within RR42_RS29860 at 2065.317 kb on - strand, within RR42_RS29860 at 2065.471 kb on - strand, within RR42_RS29860 at 2065.472 kb on + strand, within RR42_RS29860 at 2065.472 kb on + strand, within RR42_RS29860 at 2065.473 kb on - strand, within RR42_RS29860 at 2065.473 kb on - strand, within RR42_RS29860 at 2065.536 kb on - strand, within RR42_RS29860 at 2065.585 kb on + strand, within RR42_RS29860 at 2065.585 kb on + strand, within RR42_RS29860 at 2065.586 kb on - strand, within RR42_RS29860 at 2065.600 kb on + strand, within RR42_RS29860 at 2065.600 kb on + strand, within RR42_RS29860 at 2065.601 kb on - strand, within RR42_RS29860 at 2065.641 kb on + strand, within RR42_RS29860 at 2065.642 kb on - strand, within RR42_RS29860 at 2065.716 kb on + strand at 2065.716 kb on + strand at 2065.716 kb on + strand at 2065.722 kb on + strand at 2065.795 kb on + strand at 2065.795 kb on + strand at 2065.795 kb on + strand at 2065.795 kb on + strand at 2065.795 kb on + strand at 2065.819 kb on + strand at 2065.819 kb on + strand at 2065.819 kb on + strand at 2065.842 kb on + strand at 2065.842 kb on + strand at 2065.843 kb on - strand at 2065.843 kb on - strand at 2065.879 kb on + strand at 2065.880 kb on - strand at 2065.901 kb on - strand at 2065.902 kb on + strand at 2065.903 kb on - strand at 2065.903 kb on - strand at 2066.009 kb on + strand at 2066.009 kb on + strand at 2066.010 kb on - strand at 2066.053 kb on + strand at 2066.053 kb on + strand at 2066.056 kb on + strand at 2066.056 kb on + strand at 2066.056 kb on + strand at 2066.093 kb on + strand at 2066.093 kb on + strand at 2066.219 kb on + strand, within RR42_RS29865 at 2066.220 kb on - strand, within RR42_RS29865 at 2066.309 kb on + strand, within RR42_RS29865 at 2066.309 kb on + strand, within RR42_RS29865 at 2066.378 kb on + strand at 2066.379 kb on - strand at 2066.379 kb on - strand at 2066.379 kb on - strand at 2066.436 kb on + strand at 2066.436 kb on + strand at 2066.462 kb on + strand at 2066.489 kb on + strand at 2066.490 kb on - strand at 2066.537 kb on - strand at 2066.539 kb on - strand at 2066.570 kb on - strand at 2066.665 kb on + strand at 2066.809 kb on + strand at 2066.810 kb on - strand at 2066.810 kb on - strand at 2066.836 kb on + strand at 2066.836 kb on + strand at 2066.836 kb on + strand at 2066.852 kb on + strand at 2066.852 kb on + strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.853 kb on - strand at 2066.857 kb on + strand at 2066.858 kb on - strand at 2066.858 kb on - strand at 2066.902 kb on - strand, within RR42_RS29870 at 2066.902 kb on - strand, within RR42_RS29870 at 2066.902 kb on - strand, within RR42_RS29870 at 2066.910 kb on + strand, within RR42_RS29870 at 2067.050 kb on + strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.051 kb on - strand, within RR42_RS29870 at 2067.111 kb on + strand, within RR42_RS29870 at 2067.112 kb on - strand, within RR42_RS29870 at 2067.276 kb on + strand, within RR42_RS29870 at 2067.277 kb on - strand, within RR42_RS29870 at 2067.280 kb on + strand at 2067.280 kb on + strand at 2067.280 kb on + strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.281 kb on - strand at 2067.323 kb on - strand at 2067.346 kb on + strand at 2067.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,065,189 + RR42_RS29860 0.57 +0.5 2,065,190 - RR42_RS29860 0.57 -0.1 2,065,190 - RR42_RS29860 0.57 -0.5 2,065,190 - RR42_RS29860 0.57 +0.7 2,065,214 + RR42_RS29860 0.59 -0.6 2,065,214 + RR42_RS29860 0.59 -0.6 2,065,214 + RR42_RS29860 0.59 +0.3 2,065,215 - RR42_RS29860 0.59 +0.0 2,065,215 - RR42_RS29860 0.59 +0.2 2,065,215 - RR42_RS29860 0.59 -1.8 2,065,215 - RR42_RS29860 0.59 -0.5 2,065,255 + RR42_RS29860 0.62 +0.4 2,065,255 + RR42_RS29860 0.62 -0.5 2,065,255 + RR42_RS29860 0.62 -2.7 2,065,260 + RR42_RS29860 0.62 +0.0 2,065,261 - RR42_RS29860 0.62 -1.4 2,065,261 - RR42_RS29860 0.62 -0.2 2,065,279 - RR42_RS29860 0.64 -1.0 2,065,316 + RR42_RS29860 0.66 -0.8 2,065,316 + RR42_RS29860 0.66 +0.8 2,065,316 + RR42_RS29860 0.66 +0.4 2,065,316 + RR42_RS29860 0.66 -0.9 2,065,317 - RR42_RS29860 0.66 -0.2 2,065,317 - RR42_RS29860 0.66 -1.1 2,065,471 - RR42_RS29860 0.77 +0.2 2,065,472 + RR42_RS29860 0.77 -0.4 2,065,472 + RR42_RS29860 0.77 -0.1 2,065,473 - RR42_RS29860 0.77 -0.3 2,065,473 - RR42_RS29860 0.77 +1.7 2,065,536 - RR42_RS29860 0.82 +0.9 2,065,585 + RR42_RS29860 0.85 -0.2 2,065,585 + RR42_RS29860 0.85 -1.1 2,065,586 - RR42_RS29860 0.85 +0.3 2,065,600 + RR42_RS29860 0.86 +0.0 2,065,600 + RR42_RS29860 0.86 +0.5 2,065,601 - RR42_RS29860 0.86 -0.1 2,065,641 + RR42_RS29860 0.89 -0.3 2,065,642 - RR42_RS29860 0.89 +0.1 2,065,716 + +0.3 2,065,716 + +0.0 2,065,716 + +0.9 2,065,722 + +0.5 2,065,795 + -0.3 2,065,795 + +0.3 2,065,795 + -0.9 2,065,795 + -0.1 2,065,795 + -0.6 2,065,819 + +1.5 2,065,819 + -0.1 2,065,819 + -0.4 2,065,842 + -1.1 2,065,842 + -0.4 2,065,843 - +0.8 2,065,843 - -0.9 2,065,879 + +1.5 2,065,880 - +0.8 2,065,901 - -0.9 2,065,902 + +0.3 2,065,903 - +0.9 2,065,903 - +1.0 2,066,009 + -1.1 2,066,009 + +0.4 2,066,010 - -0.4 2,066,053 + +0.3 2,066,053 + +0.2 2,066,056 + +1.2 2,066,056 + +1.0 2,066,056 + -2.0 2,066,093 + -2.3 2,066,093 + -0.2 2,066,219 + RR42_RS29865 0.31 -1.3 2,066,220 - RR42_RS29865 0.31 -1.1 2,066,309 + RR42_RS29865 0.66 +0.3 2,066,309 + RR42_RS29865 0.66 -0.3 2,066,378 + -0.3 2,066,379 - -1.3 2,066,379 - +0.8 2,066,379 - +0.2 2,066,436 + -1.1 2,066,436 + -0.5 2,066,462 + +0.3 2,066,489 + -0.3 2,066,490 - +0.1 2,066,537 - -0.1 2,066,539 - +1.6 2,066,570 - -0.5 2,066,665 + +0.4 2,066,809 + -0.3 2,066,810 - -0.7 2,066,810 - -1.1 2,066,836 + -0.9 2,066,836 + +0.2 2,066,836 + +0.4 2,066,852 + +0.9 2,066,852 + -0.9 2,066,853 - -0.2 2,066,853 - -2.6 2,066,853 - -0.2 2,066,853 - +0.0 2,066,853 - +0.1 2,066,857 + +0.1 2,066,858 - +0.2 2,066,858 - +0.1 2,066,902 - RR42_RS29870 0.18 +0.8 2,066,902 - RR42_RS29870 0.18 -1.8 2,066,902 - RR42_RS29870 0.18 +1.0 2,066,910 + RR42_RS29870 0.19 -0.8 2,067,050 + RR42_RS29870 0.46 -0.2 2,067,051 - RR42_RS29870 0.46 +0.3 2,067,051 - RR42_RS29870 0.46 +0.3 2,067,051 - RR42_RS29870 0.46 +1.0 2,067,111 + RR42_RS29870 0.58 +0.1 2,067,112 - RR42_RS29870 0.58 +0.1 2,067,276 + RR42_RS29870 0.89 +0.3 2,067,277 - RR42_RS29870 0.89 -1.3 2,067,280 + -0.7 2,067,280 + +0.3 2,067,280 + -0.6 2,067,281 - +0.5 2,067,281 - -0.6 2,067,281 - -0.5 2,067,281 - -0.0 2,067,281 - +0.3 2,067,323 - +0.3 2,067,346 + -1.0 2,067,347 - -0.1
Or see this region's nucleotide sequence