Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS29465

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS29460 and RR42_RS29465 are separated by 43 nucleotidesRR42_RS29465 and RR42_RS29470 are separated by 53 nucleotides RR42_RS29460: RR42_RS29460 - fatty-acid--CoA ligase, at 1,963,852 to 1,965,681 _RS29460 RR42_RS29465: RR42_RS29465 - enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, at 1,965,725 to 1,968,010 _RS29465 RR42_RS29470: RR42_RS29470 - ABC transporter substrate-binding protein, at 1,968,064 to 1,969,236 _RS29470 Position (kb) 1965 1966 1967 1968 1969Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 1964.965 kb on + strand, within RR42_RS29460at 1964.965 kb on + strand, within RR42_RS29460at 1964.966 kb on - strand, within RR42_RS29460at 1964.966 kb on - strand, within RR42_RS29460at 1964.966 kb on - strand, within RR42_RS29460at 1964.966 kb on - strand, within RR42_RS29460at 1964.966 kb on - strand, within RR42_RS29460at 1964.968 kb on - strand, within RR42_RS29460at 1964.968 kb on - strand, within RR42_RS29460at 1965.027 kb on + strand, within RR42_RS29460at 1965.085 kb on - strand, within RR42_RS29460at 1965.148 kb on + strand, within RR42_RS29460at 1965.236 kb on - strand, within RR42_RS29460at 1965.236 kb on - strand, within RR42_RS29460at 1965.239 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.241 kb on + strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.242 kb on - strand, within RR42_RS29460at 1965.244 kb on - strand, within RR42_RS29460at 1965.334 kb on + strand, within RR42_RS29460at 1965.334 kb on + strand, within RR42_RS29460at 1965.334 kb on + strand, within RR42_RS29460at 1965.334 kb on + strand, within RR42_RS29460at 1965.335 kb on - strand, within RR42_RS29460at 1965.335 kb on - strand, within RR42_RS29460at 1965.335 kb on - strand, within RR42_RS29460at 1965.335 kb on - strand, within RR42_RS29460at 1965.373 kb on + strand, within RR42_RS29460at 1965.451 kb on + strand, within RR42_RS29460at 1965.452 kb on - strand, within RR42_RS29460at 1965.452 kb on - strand, within RR42_RS29460at 1965.637 kb on + strandat 1965.638 kb on - strandat 1965.638 kb on - strandat 1965.661 kb on + strandat 1965.662 kb on - strandat 1965.662 kb on - strandat 1965.971 kb on + strand, within RR42_RS29465at 1965.971 kb on + strand, within RR42_RS29465at 1965.972 kb on - strand, within RR42_RS29465at 1965.972 kb on - strand, within RR42_RS29465at 1965.979 kb on + strand, within RR42_RS29465at 1966.424 kb on + strand, within RR42_RS29465at 1966.628 kb on - strand, within RR42_RS29465at 1966.763 kb on + strand, within RR42_RS29465at 1966.764 kb on - strand, within RR42_RS29465at 1966.791 kb on + strand, within RR42_RS29465at 1966.792 kb on - strand, within RR42_RS29465at 1966.966 kb on + strand, within RR42_RS29465at 1966.966 kb on + strand, within RR42_RS29465at 1966.967 kb on - strand, within RR42_RS29465at 1966.967 kb on - strand, within RR42_RS29465at 1966.967 kb on - strand, within RR42_RS29465at 1966.967 kb on - strand, within RR42_RS29465at 1966.967 kb on - strand, within RR42_RS29465at 1967.201 kb on + strand, within RR42_RS29465at 1967.401 kb on + strand, within RR42_RS29465at 1967.401 kb on + strand, within RR42_RS29465at 1967.402 kb on - strand, within RR42_RS29465at 1967.451 kb on - strand, within RR42_RS29465at 1967.666 kb on + strand, within RR42_RS29465at 1967.751 kb on - strand, within RR42_RS29465at 1967.775 kb on - strand, within RR42_RS29465at 1967.775 kb on - strand, within RR42_RS29465at 1967.775 kb on - strand, within RR42_RS29465at 1967.781 kb on - strand, within RR42_RS29465at 1967.781 kb on - strand, within RR42_RS29465at 1967.918 kb on - strandat 1967.932 kb on + strandat 1967.933 kb on - strandat 1967.933 kb on - strandat 1968.174 kb on + strandat 1968.174 kb on + strandat 1968.174 kb on + strandat 1968.174 kb on + strandat 1968.175 kb on - strandat 1968.196 kb on + strand, within RR42_RS29470at 1968.196 kb on + strand, within RR42_RS29470at 1968.196 kb on + strand, within RR42_RS29470at 1968.197 kb on - strand, within RR42_RS29470at 1968.393 kb on + strand, within RR42_RS29470at 1968.466 kb on + strand, within RR42_RS29470at 1968.466 kb on + strand, within RR42_RS29470at 1968.466 kb on + strand, within RR42_RS29470at 1968.466 kb on + strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.467 kb on - strand, within RR42_RS29470at 1968.484 kb on + strand, within RR42_RS29470at 1968.484 kb on + strand, within RR42_RS29470at 1968.485 kb on - strand, within RR42_RS29470at 1968.485 kb on - strand, within RR42_RS29470at 1968.485 kb on - strand, within RR42_RS29470at 1968.538 kb on + strand, within RR42_RS29470at 1968.538 kb on + strand, within RR42_RS29470at 1968.539 kb on - strand, within RR42_RS29470at 1968.539 kb on - strand, within RR42_RS29470at 1968.539 kb on - strand, within RR42_RS29470at 1968.539 kb on - strand, within RR42_RS29470at 1968.580 kb on + strand, within RR42_RS29470at 1968.580 kb on + strand, within RR42_RS29470at 1968.581 kb on - strand, within RR42_RS29470at 1968.629 kb on - strand, within RR42_RS29470at 1968.670 kb on + strand, within RR42_RS29470at 1968.670 kb on + strand, within RR42_RS29470at 1968.671 kb on - strand, within RR42_RS29470at 1968.671 kb on - strand, within RR42_RS29470at 1968.833 kb on - strand, within RR42_RS29470at 1968.907 kb on + strand, within RR42_RS29470at 1968.953 kb on - strand, within RR42_RS29470at 1968.985 kb on + strand, within RR42_RS29470at 1968.985 kb on + strand, within RR42_RS29470at 1968.986 kb on - strand, within RR42_RS29470at 1969.000 kb on + strand, within RR42_RS29470at 1969.010 kb on - strand, within RR42_RS29470

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,964,965 + RR42_RS29460 0.61 +0.0
1,964,965 + RR42_RS29460 0.61 -0.6
1,964,966 - RR42_RS29460 0.61 -0.0
1,964,966 - RR42_RS29460 0.61 -1.3
1,964,966 - RR42_RS29460 0.61 +0.1
1,964,966 - RR42_RS29460 0.61 -0.3
1,964,966 - RR42_RS29460 0.61 -0.0
1,964,968 - RR42_RS29460 0.61 +1.0
1,964,968 - RR42_RS29460 0.61 -1.3
1,965,027 + RR42_RS29460 0.64 +0.7
1,965,085 - RR42_RS29460 0.67 -0.3
1,965,148 + RR42_RS29460 0.71 -0.3
1,965,236 - RR42_RS29460 0.76 +0.0
1,965,236 - RR42_RS29460 0.76 -0.5
1,965,239 + RR42_RS29460 0.76 -0.5
1,965,241 + RR42_RS29460 0.76 -3.0
1,965,241 + RR42_RS29460 0.76 +1.8
1,965,241 + RR42_RS29460 0.76 +0.3
1,965,241 + RR42_RS29460 0.76 -0.5
1,965,241 + RR42_RS29460 0.76 -1.0
1,965,241 + RR42_RS29460 0.76 +1.1
1,965,242 - RR42_RS29460 0.76 -0.4
1,965,242 - RR42_RS29460 0.76 -1.3
1,965,242 - RR42_RS29460 0.76 +0.9
1,965,242 - RR42_RS29460 0.76 -0.1
1,965,242 - RR42_RS29460 0.76 +0.7
1,965,242 - RR42_RS29460 0.76 +1.0
1,965,242 - RR42_RS29460 0.76 -0.7
1,965,244 - RR42_RS29460 0.76 -0.1
1,965,334 + RR42_RS29460 0.81 -0.5
1,965,334 + RR42_RS29460 0.81 -0.3
1,965,334 + RR42_RS29460 0.81 -0.8
1,965,334 + RR42_RS29460 0.81 -0.0
1,965,335 - RR42_RS29460 0.81 -0.4
1,965,335 - RR42_RS29460 0.81 +0.0
1,965,335 - RR42_RS29460 0.81 -0.3
1,965,335 - RR42_RS29460 0.81 -1.8
1,965,373 + RR42_RS29460 0.83 -0.7
1,965,451 + RR42_RS29460 0.87 -0.0
1,965,452 - RR42_RS29460 0.87 +0.1
1,965,452 - RR42_RS29460 0.87 -1.4
1,965,637 + +0.1
1,965,638 - +0.0
1,965,638 - -0.4
1,965,661 + -1.7
1,965,662 - -0.3
1,965,662 - -1.3
1,965,971 + RR42_RS29465 0.11 -0.6
1,965,971 + RR42_RS29465 0.11 -1.2
1,965,972 - RR42_RS29465 0.11 +0.7
1,965,972 - RR42_RS29465 0.11 +0.9
1,965,979 + RR42_RS29465 0.11 +0.2
1,966,424 + RR42_RS29465 0.31 +1.7
1,966,628 - RR42_RS29465 0.40 +0.2
1,966,763 + RR42_RS29465 0.45 +0.5
1,966,764 - RR42_RS29465 0.45 -0.2
1,966,791 + RR42_RS29465 0.47 -0.6
1,966,792 - RR42_RS29465 0.47 +0.8
1,966,966 + RR42_RS29465 0.54 -0.1
1,966,966 + RR42_RS29465 0.54 -0.9
1,966,967 - RR42_RS29465 0.54 +0.0
1,966,967 - RR42_RS29465 0.54 -0.4
1,966,967 - RR42_RS29465 0.54 +0.8
1,966,967 - RR42_RS29465 0.54 +1.3
1,966,967 - RR42_RS29465 0.54 +0.3
1,967,201 + RR42_RS29465 0.65 -0.5
1,967,401 + RR42_RS29465 0.73 -0.1
1,967,401 + RR42_RS29465 0.73 +1.3
1,967,402 - RR42_RS29465 0.73 -0.3
1,967,451 - RR42_RS29465 0.76 -0.3
1,967,666 + RR42_RS29465 0.85 -0.4
1,967,751 - RR42_RS29465 0.89 +0.2
1,967,775 - RR42_RS29465 0.90 +0.7
1,967,775 - RR42_RS29465 0.90 -0.8
1,967,775 - RR42_RS29465 0.90 -0.8
1,967,781 - RR42_RS29465 0.90 +0.2
1,967,781 - RR42_RS29465 0.90 +0.1
1,967,918 - +0.3
1,967,932 + -1.1
1,967,933 - +1.1
1,967,933 - -2.0
1,968,174 + +0.2
1,968,174 + +0.6
1,968,174 + -0.3
1,968,174 + -0.3
1,968,175 - +0.8
1,968,196 + RR42_RS29470 0.11 +0.3
1,968,196 + RR42_RS29470 0.11 -0.0
1,968,196 + RR42_RS29470 0.11 +0.0
1,968,197 - RR42_RS29470 0.11 -0.1
1,968,393 + RR42_RS29470 0.28 +2.0
1,968,466 + RR42_RS29470 0.34 +0.6
1,968,466 + RR42_RS29470 0.34 -0.2
1,968,466 + RR42_RS29470 0.34 +0.8
1,968,466 + RR42_RS29470 0.34 -0.3
1,968,467 - RR42_RS29470 0.34 +1.0
1,968,467 - RR42_RS29470 0.34 +2.1
1,968,467 - RR42_RS29470 0.34 +1.0
1,968,467 - RR42_RS29470 0.34 -0.8
1,968,467 - RR42_RS29470 0.34 -0.3
1,968,467 - RR42_RS29470 0.34 -0.6
1,968,467 - RR42_RS29470 0.34 +0.4
1,968,467 - RR42_RS29470 0.34 -0.0
1,968,484 + RR42_RS29470 0.36 +0.0
1,968,484 + RR42_RS29470 0.36 -0.1
1,968,485 - RR42_RS29470 0.36 -3.0
1,968,485 - RR42_RS29470 0.36 +0.2
1,968,485 - RR42_RS29470 0.36 -0.3
1,968,538 + RR42_RS29470 0.40 +0.6
1,968,538 + RR42_RS29470 0.40 +0.2
1,968,539 - RR42_RS29470 0.40 -0.7
1,968,539 - RR42_RS29470 0.40 -0.8
1,968,539 - RR42_RS29470 0.40 +1.2
1,968,539 - RR42_RS29470 0.40 -0.9
1,968,580 + RR42_RS29470 0.44 -2.5
1,968,580 + RR42_RS29470 0.44 +0.0
1,968,581 - RR42_RS29470 0.44 +0.7
1,968,629 - RR42_RS29470 0.48 +0.2
1,968,670 + RR42_RS29470 0.52 -0.1
1,968,670 + RR42_RS29470 0.52 +0.9
1,968,671 - RR42_RS29470 0.52 -5.6
1,968,671 - RR42_RS29470 0.52 -0.2
1,968,833 - RR42_RS29470 0.66 -0.7
1,968,907 + RR42_RS29470 0.72 +1.7
1,968,953 - RR42_RS29470 0.76 +0.3
1,968,985 + RR42_RS29470 0.79 -0.7
1,968,985 + RR42_RS29470 0.79 +0.3
1,968,986 - RR42_RS29470 0.79 -2.0
1,969,000 + RR42_RS29470 0.80 +0.6
1,969,010 - RR42_RS29470 0.81 -0.6

Or see this region's nucleotide sequence