Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29460 and RR42_RS29465 are separated by 43 nucleotides RR42_RS29465 and RR42_RS29470 are separated by 53 nucleotides
RR42_RS29460: RR42_RS29460 - fatty-acid--CoA ligase, at 1,963,852 to 1,965,681
_RS29460
RR42_RS29465: RR42_RS29465 - enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, at 1,965,725 to 1,968,010
_RS29465
RR42_RS29470: RR42_RS29470 - ABC transporter substrate-binding protein, at 1,968,064 to 1,969,236
_RS29470
Position (kb)
1965
1966
1967
1968
1969 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1964.965 kb on + strand, within RR42_RS29460 at 1964.965 kb on + strand, within RR42_RS29460 at 1964.966 kb on - strand, within RR42_RS29460 at 1964.966 kb on - strand, within RR42_RS29460 at 1964.966 kb on - strand, within RR42_RS29460 at 1964.966 kb on - strand, within RR42_RS29460 at 1964.966 kb on - strand, within RR42_RS29460 at 1964.968 kb on - strand, within RR42_RS29460 at 1964.968 kb on - strand, within RR42_RS29460 at 1965.027 kb on + strand, within RR42_RS29460 at 1965.085 kb on - strand, within RR42_RS29460 at 1965.148 kb on + strand, within RR42_RS29460 at 1965.236 kb on - strand, within RR42_RS29460 at 1965.236 kb on - strand, within RR42_RS29460 at 1965.239 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.241 kb on + strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.242 kb on - strand, within RR42_RS29460 at 1965.244 kb on - strand, within RR42_RS29460 at 1965.334 kb on + strand, within RR42_RS29460 at 1965.334 kb on + strand, within RR42_RS29460 at 1965.334 kb on + strand, within RR42_RS29460 at 1965.334 kb on + strand, within RR42_RS29460 at 1965.335 kb on - strand, within RR42_RS29460 at 1965.335 kb on - strand, within RR42_RS29460 at 1965.335 kb on - strand, within RR42_RS29460 at 1965.335 kb on - strand, within RR42_RS29460 at 1965.373 kb on + strand, within RR42_RS29460 at 1965.451 kb on + strand, within RR42_RS29460 at 1965.452 kb on - strand, within RR42_RS29460 at 1965.452 kb on - strand, within RR42_RS29460 at 1965.637 kb on + strand at 1965.638 kb on - strand at 1965.638 kb on - strand at 1965.661 kb on + strand at 1965.662 kb on - strand at 1965.662 kb on - strand at 1965.971 kb on + strand, within RR42_RS29465 at 1965.971 kb on + strand, within RR42_RS29465 at 1965.972 kb on - strand, within RR42_RS29465 at 1965.972 kb on - strand, within RR42_RS29465 at 1965.979 kb on + strand, within RR42_RS29465 at 1966.424 kb on + strand, within RR42_RS29465 at 1966.628 kb on - strand, within RR42_RS29465 at 1966.763 kb on + strand, within RR42_RS29465 at 1966.764 kb on - strand, within RR42_RS29465 at 1966.791 kb on + strand, within RR42_RS29465 at 1966.792 kb on - strand, within RR42_RS29465 at 1966.966 kb on + strand, within RR42_RS29465 at 1966.966 kb on + strand, within RR42_RS29465 at 1966.967 kb on - strand, within RR42_RS29465 at 1966.967 kb on - strand, within RR42_RS29465 at 1966.967 kb on - strand, within RR42_RS29465 at 1966.967 kb on - strand, within RR42_RS29465 at 1966.967 kb on - strand, within RR42_RS29465 at 1967.201 kb on + strand, within RR42_RS29465 at 1967.401 kb on + strand, within RR42_RS29465 at 1967.401 kb on + strand, within RR42_RS29465 at 1967.402 kb on - strand, within RR42_RS29465 at 1967.451 kb on - strand, within RR42_RS29465 at 1967.666 kb on + strand, within RR42_RS29465 at 1967.751 kb on - strand, within RR42_RS29465 at 1967.775 kb on - strand, within RR42_RS29465 at 1967.775 kb on - strand, within RR42_RS29465 at 1967.775 kb on - strand, within RR42_RS29465 at 1967.781 kb on - strand, within RR42_RS29465 at 1967.781 kb on - strand, within RR42_RS29465 at 1967.918 kb on - strand at 1967.932 kb on + strand at 1967.933 kb on - strand at 1967.933 kb on - strand at 1968.174 kb on + strand at 1968.174 kb on + strand at 1968.174 kb on + strand at 1968.174 kb on + strand at 1968.175 kb on - strand at 1968.196 kb on + strand, within RR42_RS29470 at 1968.196 kb on + strand, within RR42_RS29470 at 1968.196 kb on + strand, within RR42_RS29470 at 1968.197 kb on - strand, within RR42_RS29470 at 1968.393 kb on + strand, within RR42_RS29470 at 1968.466 kb on + strand, within RR42_RS29470 at 1968.466 kb on + strand, within RR42_RS29470 at 1968.466 kb on + strand, within RR42_RS29470 at 1968.466 kb on + strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.467 kb on - strand, within RR42_RS29470 at 1968.484 kb on + strand, within RR42_RS29470 at 1968.484 kb on + strand, within RR42_RS29470 at 1968.485 kb on - strand, within RR42_RS29470 at 1968.485 kb on - strand, within RR42_RS29470 at 1968.485 kb on - strand, within RR42_RS29470 at 1968.538 kb on + strand, within RR42_RS29470 at 1968.538 kb on + strand, within RR42_RS29470 at 1968.539 kb on - strand, within RR42_RS29470 at 1968.539 kb on - strand, within RR42_RS29470 at 1968.539 kb on - strand, within RR42_RS29470 at 1968.539 kb on - strand, within RR42_RS29470 at 1968.580 kb on + strand, within RR42_RS29470 at 1968.580 kb on + strand, within RR42_RS29470 at 1968.581 kb on - strand, within RR42_RS29470 at 1968.629 kb on - strand, within RR42_RS29470 at 1968.670 kb on + strand, within RR42_RS29470 at 1968.670 kb on + strand, within RR42_RS29470 at 1968.671 kb on - strand, within RR42_RS29470 at 1968.671 kb on - strand, within RR42_RS29470 at 1968.833 kb on - strand, within RR42_RS29470 at 1968.907 kb on + strand, within RR42_RS29470 at 1968.953 kb on - strand, within RR42_RS29470 at 1968.985 kb on + strand, within RR42_RS29470 at 1968.985 kb on + strand, within RR42_RS29470 at 1968.986 kb on - strand, within RR42_RS29470 at 1969.000 kb on + strand, within RR42_RS29470 at 1969.010 kb on - strand, within RR42_RS29470
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,964,965 + RR42_RS29460 0.61 +0.0 1,964,965 + RR42_RS29460 0.61 -0.6 1,964,966 - RR42_RS29460 0.61 -0.0 1,964,966 - RR42_RS29460 0.61 -1.3 1,964,966 - RR42_RS29460 0.61 +0.1 1,964,966 - RR42_RS29460 0.61 -0.3 1,964,966 - RR42_RS29460 0.61 -0.0 1,964,968 - RR42_RS29460 0.61 +1.0 1,964,968 - RR42_RS29460 0.61 -1.3 1,965,027 + RR42_RS29460 0.64 +0.7 1,965,085 - RR42_RS29460 0.67 -0.3 1,965,148 + RR42_RS29460 0.71 -0.3 1,965,236 - RR42_RS29460 0.76 +0.0 1,965,236 - RR42_RS29460 0.76 -0.5 1,965,239 + RR42_RS29460 0.76 -0.5 1,965,241 + RR42_RS29460 0.76 -3.0 1,965,241 + RR42_RS29460 0.76 +1.8 1,965,241 + RR42_RS29460 0.76 +0.3 1,965,241 + RR42_RS29460 0.76 -0.5 1,965,241 + RR42_RS29460 0.76 -1.0 1,965,241 + RR42_RS29460 0.76 +1.1 1,965,242 - RR42_RS29460 0.76 -0.4 1,965,242 - RR42_RS29460 0.76 -1.3 1,965,242 - RR42_RS29460 0.76 +0.9 1,965,242 - RR42_RS29460 0.76 -0.1 1,965,242 - RR42_RS29460 0.76 +0.7 1,965,242 - RR42_RS29460 0.76 +1.0 1,965,242 - RR42_RS29460 0.76 -0.7 1,965,244 - RR42_RS29460 0.76 -0.1 1,965,334 + RR42_RS29460 0.81 -0.5 1,965,334 + RR42_RS29460 0.81 -0.3 1,965,334 + RR42_RS29460 0.81 -0.8 1,965,334 + RR42_RS29460 0.81 -0.0 1,965,335 - RR42_RS29460 0.81 -0.4 1,965,335 - RR42_RS29460 0.81 +0.0 1,965,335 - RR42_RS29460 0.81 -0.3 1,965,335 - RR42_RS29460 0.81 -1.8 1,965,373 + RR42_RS29460 0.83 -0.7 1,965,451 + RR42_RS29460 0.87 -0.0 1,965,452 - RR42_RS29460 0.87 +0.1 1,965,452 - RR42_RS29460 0.87 -1.4 1,965,637 + +0.1 1,965,638 - +0.0 1,965,638 - -0.4 1,965,661 + -1.7 1,965,662 - -0.3 1,965,662 - -1.3 1,965,971 + RR42_RS29465 0.11 -0.6 1,965,971 + RR42_RS29465 0.11 -1.2 1,965,972 - RR42_RS29465 0.11 +0.7 1,965,972 - RR42_RS29465 0.11 +0.9 1,965,979 + RR42_RS29465 0.11 +0.2 1,966,424 + RR42_RS29465 0.31 +1.7 1,966,628 - RR42_RS29465 0.40 +0.2 1,966,763 + RR42_RS29465 0.45 +0.5 1,966,764 - RR42_RS29465 0.45 -0.2 1,966,791 + RR42_RS29465 0.47 -0.6 1,966,792 - RR42_RS29465 0.47 +0.8 1,966,966 + RR42_RS29465 0.54 -0.1 1,966,966 + RR42_RS29465 0.54 -0.9 1,966,967 - RR42_RS29465 0.54 +0.0 1,966,967 - RR42_RS29465 0.54 -0.4 1,966,967 - RR42_RS29465 0.54 +0.8 1,966,967 - RR42_RS29465 0.54 +1.3 1,966,967 - RR42_RS29465 0.54 +0.3 1,967,201 + RR42_RS29465 0.65 -0.5 1,967,401 + RR42_RS29465 0.73 -0.1 1,967,401 + RR42_RS29465 0.73 +1.3 1,967,402 - RR42_RS29465 0.73 -0.3 1,967,451 - RR42_RS29465 0.76 -0.3 1,967,666 + RR42_RS29465 0.85 -0.4 1,967,751 - RR42_RS29465 0.89 +0.2 1,967,775 - RR42_RS29465 0.90 +0.7 1,967,775 - RR42_RS29465 0.90 -0.8 1,967,775 - RR42_RS29465 0.90 -0.8 1,967,781 - RR42_RS29465 0.90 +0.2 1,967,781 - RR42_RS29465 0.90 +0.1 1,967,918 - +0.3 1,967,932 + -1.1 1,967,933 - +1.1 1,967,933 - -2.0 1,968,174 + +0.2 1,968,174 + +0.6 1,968,174 + -0.3 1,968,174 + -0.3 1,968,175 - +0.8 1,968,196 + RR42_RS29470 0.11 +0.3 1,968,196 + RR42_RS29470 0.11 -0.0 1,968,196 + RR42_RS29470 0.11 +0.0 1,968,197 - RR42_RS29470 0.11 -0.1 1,968,393 + RR42_RS29470 0.28 +2.0 1,968,466 + RR42_RS29470 0.34 +0.6 1,968,466 + RR42_RS29470 0.34 -0.2 1,968,466 + RR42_RS29470 0.34 +0.8 1,968,466 + RR42_RS29470 0.34 -0.3 1,968,467 - RR42_RS29470 0.34 +1.0 1,968,467 - RR42_RS29470 0.34 +2.1 1,968,467 - RR42_RS29470 0.34 +1.0 1,968,467 - RR42_RS29470 0.34 -0.8 1,968,467 - RR42_RS29470 0.34 -0.3 1,968,467 - RR42_RS29470 0.34 -0.6 1,968,467 - RR42_RS29470 0.34 +0.4 1,968,467 - RR42_RS29470 0.34 -0.0 1,968,484 + RR42_RS29470 0.36 +0.0 1,968,484 + RR42_RS29470 0.36 -0.1 1,968,485 - RR42_RS29470 0.36 -3.0 1,968,485 - RR42_RS29470 0.36 +0.2 1,968,485 - RR42_RS29470 0.36 -0.3 1,968,538 + RR42_RS29470 0.40 +0.6 1,968,538 + RR42_RS29470 0.40 +0.2 1,968,539 - RR42_RS29470 0.40 -0.7 1,968,539 - RR42_RS29470 0.40 -0.8 1,968,539 - RR42_RS29470 0.40 +1.2 1,968,539 - RR42_RS29470 0.40 -0.9 1,968,580 + RR42_RS29470 0.44 -2.5 1,968,580 + RR42_RS29470 0.44 +0.0 1,968,581 - RR42_RS29470 0.44 +0.7 1,968,629 - RR42_RS29470 0.48 +0.2 1,968,670 + RR42_RS29470 0.52 -0.1 1,968,670 + RR42_RS29470 0.52 +0.9 1,968,671 - RR42_RS29470 0.52 -5.6 1,968,671 - RR42_RS29470 0.52 -0.2 1,968,833 - RR42_RS29470 0.66 -0.7 1,968,907 + RR42_RS29470 0.72 +1.7 1,968,953 - RR42_RS29470 0.76 +0.3 1,968,985 + RR42_RS29470 0.79 -0.7 1,968,985 + RR42_RS29470 0.79 +0.3 1,968,986 - RR42_RS29470 0.79 -2.0 1,969,000 + RR42_RS29470 0.80 +0.6 1,969,010 - RR42_RS29470 0.81 -0.6
Or see this region's nucleotide sequence