Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29415 and RR42_RS29420 are separated by 39 nucleotides RR42_RS29420 and RR42_RS29425 are separated by 85 nucleotides
RR42_RS29415: RR42_RS29415 - acyl-CoA dehydrogenase, at 1,953,523 to 1,954,677
_RS29415
RR42_RS29420: RR42_RS29420 - 3-methylcrotonyl-CoA carboxylase, at 1,954,717 to 1,956,717
_RS29420
RR42_RS29425: RR42_RS29425 - enoyl-CoA hydratase, at 1,956,803 to 1,957,588
_RS29425
Position (kb)
1954
1955
1956
1957 Strain fitness (log2 ratio)
-2
-1
0
1 at 1953.721 kb on + strand, within RR42_RS29415 at 1953.722 kb on - strand, within RR42_RS29415 at 1953.877 kb on + strand, within RR42_RS29415 at 1953.877 kb on + strand, within RR42_RS29415 at 1953.878 kb on - strand, within RR42_RS29415 at 1953.878 kb on - strand, within RR42_RS29415 at 1953.964 kb on + strand, within RR42_RS29415 at 1953.964 kb on + strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1953.965 kb on - strand, within RR42_RS29415 at 1954.036 kb on - strand, within RR42_RS29415 at 1954.036 kb on - strand, within RR42_RS29415 at 1954.159 kb on + strand, within RR42_RS29415 at 1954.160 kb on - strand, within RR42_RS29415 at 1954.218 kb on + strand, within RR42_RS29415 at 1954.218 kb on + strand, within RR42_RS29415 at 1954.219 kb on - strand, within RR42_RS29415 at 1954.222 kb on + strand, within RR42_RS29415 at 1954.222 kb on + strand, within RR42_RS29415 at 1954.244 kb on + strand, within RR42_RS29415 at 1954.245 kb on - strand, within RR42_RS29415 at 1954.245 kb on - strand, within RR42_RS29415 at 1954.312 kb on + strand, within RR42_RS29415 at 1954.312 kb on + strand, within RR42_RS29415 at 1954.313 kb on - strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.369 kb on + strand, within RR42_RS29415 at 1954.370 kb on - strand, within RR42_RS29415 at 1954.370 kb on - strand, within RR42_RS29415 at 1954.549 kb on + strand, within RR42_RS29415 at 1954.550 kb on - strand, within RR42_RS29415 at 1954.550 kb on - strand, within RR42_RS29415 at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.879 kb on + strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.880 kb on - strand at 1954.881 kb on + strand at 1954.882 kb on - strand at 1954.909 kb on + strand at 1954.910 kb on - strand at 1955.033 kb on + strand, within RR42_RS29420 at 1955.033 kb on + strand, within RR42_RS29420 at 1955.033 kb on + strand, within RR42_RS29420 at 1955.034 kb on - strand, within RR42_RS29420 at 1955.131 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.581 kb on + strand, within RR42_RS29420 at 1955.582 kb on - strand, within RR42_RS29420 at 1955.582 kb on - strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.584 kb on + strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.585 kb on - strand, within RR42_RS29420 at 1955.775 kb on + strand, within RR42_RS29420 at 1955.776 kb on - strand, within RR42_RS29420 at 1955.869 kb on + strand, within RR42_RS29420 at 1955.869 kb on + strand, within RR42_RS29420 at 1955.870 kb on - strand, within RR42_RS29420 at 1956.089 kb on + strand, within RR42_RS29420 at 1956.090 kb on - strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.127 kb on + strand, within RR42_RS29420 at 1956.241 kb on + strand, within RR42_RS29420 at 1956.242 kb on - strand, within RR42_RS29420 at 1956.309 kb on - strand, within RR42_RS29420 at 1956.787 kb on + strand at 1957.065 kb on - strand, within RR42_RS29425 at 1957.065 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.252 kb on - strand, within RR42_RS29425 at 1957.439 kb on + strand, within RR42_RS29425 at 1957.439 kb on + strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.440 kb on - strand, within RR42_RS29425 at 1957.487 kb on + strand, within RR42_RS29425 at 1957.489 kb on + strand, within RR42_RS29425 at 1957.586 kb on + strand at 1957.587 kb on - strand at 1957.587 kb on - strand at 1957.660 kb on + strand at 1957.660 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,953,721 + RR42_RS29415 0.17 +0.3 1,953,722 - RR42_RS29415 0.17 -0.1 1,953,877 + RR42_RS29415 0.31 -1.1 1,953,877 + RR42_RS29415 0.31 -0.3 1,953,878 - RR42_RS29415 0.31 +0.3 1,953,878 - RR42_RS29415 0.31 +0.3 1,953,964 + RR42_RS29415 0.38 +0.7 1,953,964 + RR42_RS29415 0.38 -2.0 1,953,965 - RR42_RS29415 0.38 -0.5 1,953,965 - RR42_RS29415 0.38 +0.1 1,953,965 - RR42_RS29415 0.38 +0.9 1,953,965 - RR42_RS29415 0.38 -0.6 1,953,965 - RR42_RS29415 0.38 +0.6 1,953,965 - RR42_RS29415 0.38 +0.2 1,954,036 - RR42_RS29415 0.44 +0.3 1,954,036 - RR42_RS29415 0.44 -0.9 1,954,159 + RR42_RS29415 0.55 +0.5 1,954,160 - RR42_RS29415 0.55 -0.1 1,954,218 + RR42_RS29415 0.60 +0.3 1,954,218 + RR42_RS29415 0.60 -0.4 1,954,219 - RR42_RS29415 0.60 +1.1 1,954,222 + RR42_RS29415 0.61 -0.2 1,954,222 + RR42_RS29415 0.61 -0.8 1,954,244 + RR42_RS29415 0.62 -1.0 1,954,245 - RR42_RS29415 0.63 +0.2 1,954,245 - RR42_RS29415 0.63 +0.3 1,954,312 + RR42_RS29415 0.68 +0.9 1,954,312 + RR42_RS29415 0.68 +0.8 1,954,313 - RR42_RS29415 0.68 +0.7 1,954,369 + RR42_RS29415 0.73 -0.8 1,954,369 + RR42_RS29415 0.73 +0.6 1,954,369 + RR42_RS29415 0.73 +0.4 1,954,370 - RR42_RS29415 0.73 +0.3 1,954,370 - RR42_RS29415 0.73 +0.1 1,954,549 + RR42_RS29415 0.89 +0.4 1,954,550 - RR42_RS29415 0.89 +1.1 1,954,550 - RR42_RS29415 0.89 -0.7 1,954,879 + -0.5 1,954,879 + -0.3 1,954,879 + -0.4 1,954,879 + -0.5 1,954,879 + -0.9 1,954,880 - -1.0 1,954,880 - +0.2 1,954,880 - -0.9 1,954,880 - -0.5 1,954,880 - -0.2 1,954,881 + +0.5 1,954,882 - +0.6 1,954,909 + +0.5 1,954,910 - -0.6 1,955,033 + RR42_RS29420 0.16 -1.1 1,955,033 + RR42_RS29420 0.16 -0.9 1,955,033 + RR42_RS29420 0.16 -0.2 1,955,034 - RR42_RS29420 0.16 -0.2 1,955,131 + RR42_RS29420 0.21 +0.3 1,955,581 + RR42_RS29420 0.43 -1.3 1,955,581 + RR42_RS29420 0.43 +0.1 1,955,581 + RR42_RS29420 0.43 -1.3 1,955,581 + RR42_RS29420 0.43 -0.2 1,955,582 - RR42_RS29420 0.43 -0.5 1,955,582 - RR42_RS29420 0.43 +1.5 1,955,584 + RR42_RS29420 0.43 -0.4 1,955,584 + RR42_RS29420 0.43 -0.3 1,955,584 + RR42_RS29420 0.43 +0.0 1,955,585 - RR42_RS29420 0.43 +0.2 1,955,585 - RR42_RS29420 0.43 -1.3 1,955,585 - RR42_RS29420 0.43 +0.3 1,955,585 - RR42_RS29420 0.43 -0.2 1,955,585 - RR42_RS29420 0.43 -1.0 1,955,775 + RR42_RS29420 0.53 +0.5 1,955,776 - RR42_RS29420 0.53 +0.7 1,955,869 + RR42_RS29420 0.58 -0.8 1,955,869 + RR42_RS29420 0.58 -0.5 1,955,870 - RR42_RS29420 0.58 +0.2 1,956,089 + RR42_RS29420 0.69 -0.1 1,956,090 - RR42_RS29420 0.69 -0.5 1,956,127 + RR42_RS29420 0.70 -0.4 1,956,127 + RR42_RS29420 0.70 +0.9 1,956,127 + RR42_RS29420 0.70 -0.9 1,956,241 + RR42_RS29420 0.76 +0.3 1,956,242 - RR42_RS29420 0.76 -0.1 1,956,309 - RR42_RS29420 0.80 +1.5 1,956,787 + +0.7 1,957,065 - RR42_RS29425 0.33 -0.5 1,957,065 - RR42_RS29425 0.33 +0.9 1,957,252 - RR42_RS29425 0.57 -1.3 1,957,252 - RR42_RS29425 0.57 +0.7 1,957,252 - RR42_RS29425 0.57 -0.2 1,957,439 + RR42_RS29425 0.81 -0.5 1,957,439 + RR42_RS29425 0.81 -0.9 1,957,440 - RR42_RS29425 0.81 +0.8 1,957,440 - RR42_RS29425 0.81 +0.8 1,957,440 - RR42_RS29425 0.81 -0.5 1,957,487 + RR42_RS29425 0.87 -0.0 1,957,489 + RR42_RS29425 0.87 +0.3 1,957,586 + -0.5 1,957,587 - -0.1 1,957,587 - -2.0 1,957,660 + +0.4 1,957,660 + +0.4
Or see this region's nucleotide sequence