Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28555

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28545 and RR42_RS28550 are separated by 10 nucleotidesRR42_RS28550 and RR42_RS28555 overlap by 4 nucleotidesRR42_RS28555 and RR42_RS28560 are separated by 449 nucleotides RR42_RS28545: RR42_RS28545 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data), at 1,759,161 to 1,760,315 _RS28545 RR42_RS28550: RR42_RS28550 - enoyl-CoA hydratase, at 1,760,326 to 1,761,096 _RS28550 RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992 _RS28555 RR42_RS28560: RR42_RS28560 - methylmalonate-semialdehyde dehydrogenase, at 1,762,442 to 1,763,962 _RS28560 Position (kb) 1761 1762Strain fitness (log2 ratio) -3 -2 -1 0 1at 1760.327 kb on + strandat 1760.328 kb on - strandat 1760.487 kb on - strand, within RR42_RS28550at 1760.793 kb on - strand, within RR42_RS28550at 1760.992 kb on + strand, within RR42_RS28550at 1761.121 kb on + strandat 1761.970 kb on - strandat 1761.984 kb on + strandat 1761.984 kb on + strandat 1761.984 kb on + strandat 1761.985 kb on - strandat 1762.042 kb on + strandat 1762.043 kb on - strandat 1762.462 kb on + strandat 1762.527 kb on + strandat 1762.551 kb on + strandat 1762.698 kb on - strand, within RR42_RS28560at 1762.772 kb on + strand, within RR42_RS28560at 1762.773 kb on - strand, within RR42_RS28560at 1762.803 kb on + strand, within RR42_RS28560at 1762.803 kb on + strand, within RR42_RS28560at 1762.803 kb on + strand, within RR42_RS28560at 1762.979 kb on + strand, within RR42_RS28560at 1762.980 kb on - strand, within RR42_RS28560

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,760,327 + +0.3
1,760,328 - -0.5
1,760,487 - RR42_RS28550 0.21 -1.8
1,760,793 - RR42_RS28550 0.61 +0.5
1,760,992 + RR42_RS28550 0.86 +0.3
1,761,121 + +0.7
1,761,970 - +0.9
1,761,984 + +0.6
1,761,984 + +0.3
1,761,984 + +0.2
1,761,985 - -1.1
1,762,042 + +0.3
1,762,043 - +0.2
1,762,462 + +0.3
1,762,527 + +0.5
1,762,551 + -0.5
1,762,698 - RR42_RS28560 0.17 -3.1
1,762,772 + RR42_RS28560 0.22 +0.8
1,762,773 - RR42_RS28560 0.22 -0.7
1,762,803 + RR42_RS28560 0.24 +1.7
1,762,803 + RR42_RS28560 0.24 -1.5
1,762,803 + RR42_RS28560 0.24 +0.9
1,762,979 + RR42_RS28560 0.35 +0.1
1,762,980 - RR42_RS28560 0.35 -0.0

Or see this region's nucleotide sequence