Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28545 and RR42_RS28550 are separated by 10 nucleotides RR42_RS28550 and RR42_RS28555 overlap by 4 nucleotides RR42_RS28555 and RR42_RS28560 are separated by 449 nucleotides
RR42_RS28545: RR42_RS28545 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 1,759,161 to 1,760,315
_RS28545
RR42_RS28550: RR42_RS28550 - enoyl-CoA hydratase, at 1,760,326 to 1,761,096
_RS28550
RR42_RS28555: RR42_RS28555 - 3-hydroxyisobutyrate dehydrogenase, at 1,761,093 to 1,761,992
_RS28555
RR42_RS28560: RR42_RS28560 - methylmalonate-semialdehyde dehydrogenase, at 1,762,442 to 1,763,962
_RS28560
Position (kb)
1761
1762 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1760.327 kb on + strand at 1760.328 kb on - strand at 1760.487 kb on - strand, within RR42_RS28550 at 1760.793 kb on - strand, within RR42_RS28550 at 1760.992 kb on + strand, within RR42_RS28550 at 1761.121 kb on + strand at 1761.970 kb on - strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.984 kb on + strand at 1761.985 kb on - strand at 1762.042 kb on + strand at 1762.043 kb on - strand at 1762.462 kb on + strand at 1762.527 kb on + strand at 1762.551 kb on + strand at 1762.698 kb on - strand, within RR42_RS28560 at 1762.772 kb on + strand, within RR42_RS28560 at 1762.773 kb on - strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.803 kb on + strand, within RR42_RS28560 at 1762.979 kb on + strand, within RR42_RS28560 at 1762.980 kb on - strand, within RR42_RS28560
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,760,327 + +0.3 1,760,328 - -0.5 1,760,487 - RR42_RS28550 0.21 -1.8 1,760,793 - RR42_RS28550 0.61 +0.5 1,760,992 + RR42_RS28550 0.86 +0.3 1,761,121 + +0.7 1,761,970 - +0.9 1,761,984 + +0.6 1,761,984 + +0.3 1,761,984 + +0.2 1,761,985 - -1.1 1,762,042 + +0.3 1,762,043 - +0.2 1,762,462 + +0.3 1,762,527 + +0.5 1,762,551 + -0.5 1,762,698 - RR42_RS28560 0.17 -3.1 1,762,772 + RR42_RS28560 0.22 +0.8 1,762,773 - RR42_RS28560 0.22 -0.7 1,762,803 + RR42_RS28560 0.24 +1.7 1,762,803 + RR42_RS28560 0.24 -1.5 1,762,803 + RR42_RS28560 0.24 +0.9 1,762,979 + RR42_RS28560 0.35 +0.1 1,762,980 - RR42_RS28560 0.35 -0.0
Or see this region's nucleotide sequence