Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS28440 and RR42_RS28445 are separated by 135 nucleotides RR42_RS28445 and RR42_RS28450 are separated by 52 nucleotides
RR42_RS28440: RR42_RS28440 - alanine racemase, at 1,739,517 to 1,740,695
_RS28440
RR42_RS28445: RR42_RS28445 - ABC transporter substrate-binding protein, at 1,740,831 to 1,741,811
_RS28445
RR42_RS28450: RR42_RS28450 - serine dehydratase, at 1,741,864 to 1,743,237
_RS28450
Position (kb)
1740
1741
1742 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1739.861 kb on + strand, within RR42_RS28440 at 1739.912 kb on - strand, within RR42_RS28440 at 1739.932 kb on + strand, within RR42_RS28440 at 1739.932 kb on + strand, within RR42_RS28440 at 1739.933 kb on - strand, within RR42_RS28440 at 1739.933 kb on - strand, within RR42_RS28440 at 1739.933 kb on - strand, within RR42_RS28440 at 1739.933 kb on - strand, within RR42_RS28440 at 1739.956 kb on + strand, within RR42_RS28440 at 1739.956 kb on + strand, within RR42_RS28440 at 1739.957 kb on - strand, within RR42_RS28440 at 1740.153 kb on + strand, within RR42_RS28440 at 1740.395 kb on + strand, within RR42_RS28440 at 1740.493 kb on - strand, within RR42_RS28440 at 1740.695 kb on + strand at 1740.695 kb on + strand at 1740.695 kb on + strand at 1741.056 kb on - strand, within RR42_RS28445 at 1741.056 kb on - strand, within RR42_RS28445 at 1741.056 kb on - strand, within RR42_RS28445 at 1741.062 kb on - strand, within RR42_RS28445 at 1741.320 kb on - strand, within RR42_RS28445 at 1741.478 kb on - strand, within RR42_RS28445 at 1741.726 kb on + strand at 1741.796 kb on + strand at 1741.796 kb on + strand at 1741.905 kb on - strand at 1741.905 kb on - strand at 1741.994 kb on + strand at 1741.995 kb on - strand at 1742.231 kb on + strand, within RR42_RS28450 at 1742.232 kb on - strand, within RR42_RS28450 at 1742.232 kb on - strand, within RR42_RS28450 at 1742.232 kb on - strand, within RR42_RS28450 at 1742.385 kb on - strand, within RR42_RS28450 at 1742.465 kb on + strand, within RR42_RS28450 at 1742.507 kb on + strand, within RR42_RS28450 at 1742.508 kb on - strand, within RR42_RS28450 at 1742.562 kb on - strand, within RR42_RS28450 at 1742.640 kb on + strand, within RR42_RS28450 at 1742.640 kb on + strand, within RR42_RS28450 at 1742.640 kb on + strand, within RR42_RS28450 at 1742.640 kb on + strand, within RR42_RS28450 at 1742.640 kb on + strand, within RR42_RS28450 at 1742.641 kb on - strand, within RR42_RS28450 at 1742.641 kb on - strand, within RR42_RS28450 at 1742.641 kb on - strand, within RR42_RS28450 at 1742.641 kb on - strand, within RR42_RS28450 at 1742.641 kb on - strand, within RR42_RS28450 at 1742.771 kb on + strand, within RR42_RS28450 at 1742.771 kb on + strand, within RR42_RS28450 at 1742.771 kb on + strand, within RR42_RS28450
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,739,861 + RR42_RS28440 0.29 -1.1 1,739,912 - RR42_RS28440 0.34 -1.7 1,739,932 + RR42_RS28440 0.35 +0.0 1,739,932 + RR42_RS28440 0.35 -0.5 1,739,933 - RR42_RS28440 0.35 -0.5 1,739,933 - RR42_RS28440 0.35 +0.1 1,739,933 - RR42_RS28440 0.35 +1.1 1,739,933 - RR42_RS28440 0.35 -0.3 1,739,956 + RR42_RS28440 0.37 +0.2 1,739,956 + RR42_RS28440 0.37 +1.0 1,739,957 - RR42_RS28440 0.37 -0.7 1,740,153 + RR42_RS28440 0.54 +0.9 1,740,395 + RR42_RS28440 0.74 +0.0 1,740,493 - RR42_RS28440 0.83 +0.0 1,740,695 + -1.8 1,740,695 + -0.7 1,740,695 + +0.9 1,741,056 - RR42_RS28445 0.23 -1.7 1,741,056 - RR42_RS28445 0.23 -0.4 1,741,056 - RR42_RS28445 0.23 -0.7 1,741,062 - RR42_RS28445 0.24 +2.9 1,741,320 - RR42_RS28445 0.50 -1.8 1,741,478 - RR42_RS28445 0.66 -0.7 1,741,726 + +0.3 1,741,796 + -0.3 1,741,796 + -0.4 1,741,905 - +1.1 1,741,905 - +0.1 1,741,994 + -0.3 1,741,995 - +0.3 1,742,231 + RR42_RS28450 0.27 +2.7 1,742,232 - RR42_RS28450 0.27 -0.4 1,742,232 - RR42_RS28450 0.27 -0.5 1,742,232 - RR42_RS28450 0.27 +0.7 1,742,385 - RR42_RS28450 0.38 -0.5 1,742,465 + RR42_RS28450 0.44 +0.5 1,742,507 + RR42_RS28450 0.47 -0.1 1,742,508 - RR42_RS28450 0.47 +0.3 1,742,562 - RR42_RS28450 0.51 +0.7 1,742,640 + RR42_RS28450 0.56 -1.0 1,742,640 + RR42_RS28450 0.56 -0.6 1,742,640 + RR42_RS28450 0.56 +0.1 1,742,640 + RR42_RS28450 0.56 +0.9 1,742,640 + RR42_RS28450 0.56 -0.1 1,742,641 - RR42_RS28450 0.57 -0.3 1,742,641 - RR42_RS28450 0.57 +0.0 1,742,641 - RR42_RS28450 0.57 +0.0 1,742,641 - RR42_RS28450 0.57 +0.7 1,742,641 - RR42_RS28450 0.57 -0.3 1,742,771 + RR42_RS28450 0.66 -0.6 1,742,771 + RR42_RS28450 0.66 -3.0 1,742,771 + RR42_RS28450 0.66 +0.0
Or see this region's nucleotide sequence