Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS28445

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS28440 and RR42_RS28445 are separated by 135 nucleotidesRR42_RS28445 and RR42_RS28450 are separated by 52 nucleotides RR42_RS28440: RR42_RS28440 - alanine racemase, at 1,739,517 to 1,740,695 _RS28440 RR42_RS28445: RR42_RS28445 - ABC transporter substrate-binding protein, at 1,740,831 to 1,741,811 _RS28445 RR42_RS28450: RR42_RS28450 - serine dehydratase, at 1,741,864 to 1,743,237 _RS28450 Position (kb) 1740 1741 1742Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1739.861 kb on + strand, within RR42_RS28440at 1739.912 kb on - strand, within RR42_RS28440at 1739.932 kb on + strand, within RR42_RS28440at 1739.932 kb on + strand, within RR42_RS28440at 1739.933 kb on - strand, within RR42_RS28440at 1739.933 kb on - strand, within RR42_RS28440at 1739.933 kb on - strand, within RR42_RS28440at 1739.933 kb on - strand, within RR42_RS28440at 1739.956 kb on + strand, within RR42_RS28440at 1739.956 kb on + strand, within RR42_RS28440at 1739.957 kb on - strand, within RR42_RS28440at 1740.153 kb on + strand, within RR42_RS28440at 1740.395 kb on + strand, within RR42_RS28440at 1740.493 kb on - strand, within RR42_RS28440at 1740.695 kb on + strandat 1740.695 kb on + strandat 1740.695 kb on + strandat 1741.056 kb on - strand, within RR42_RS28445at 1741.056 kb on - strand, within RR42_RS28445at 1741.056 kb on - strand, within RR42_RS28445at 1741.062 kb on - strand, within RR42_RS28445at 1741.320 kb on - strand, within RR42_RS28445at 1741.478 kb on - strand, within RR42_RS28445at 1741.726 kb on + strandat 1741.796 kb on + strandat 1741.796 kb on + strandat 1741.905 kb on - strandat 1741.905 kb on - strandat 1741.994 kb on + strandat 1741.995 kb on - strandat 1742.231 kb on + strand, within RR42_RS28450at 1742.232 kb on - strand, within RR42_RS28450at 1742.232 kb on - strand, within RR42_RS28450at 1742.232 kb on - strand, within RR42_RS28450at 1742.385 kb on - strand, within RR42_RS28450at 1742.465 kb on + strand, within RR42_RS28450at 1742.507 kb on + strand, within RR42_RS28450at 1742.508 kb on - strand, within RR42_RS28450at 1742.562 kb on - strand, within RR42_RS28450at 1742.640 kb on + strand, within RR42_RS28450at 1742.640 kb on + strand, within RR42_RS28450at 1742.640 kb on + strand, within RR42_RS28450at 1742.640 kb on + strand, within RR42_RS28450at 1742.640 kb on + strand, within RR42_RS28450at 1742.641 kb on - strand, within RR42_RS28450at 1742.641 kb on - strand, within RR42_RS28450at 1742.641 kb on - strand, within RR42_RS28450at 1742.641 kb on - strand, within RR42_RS28450at 1742.641 kb on - strand, within RR42_RS28450at 1742.771 kb on + strand, within RR42_RS28450at 1742.771 kb on + strand, within RR42_RS28450at 1742.771 kb on + strand, within RR42_RS28450

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,739,861 + RR42_RS28440 0.29 -1.1
1,739,912 - RR42_RS28440 0.34 -1.7
1,739,932 + RR42_RS28440 0.35 +0.0
1,739,932 + RR42_RS28440 0.35 -0.5
1,739,933 - RR42_RS28440 0.35 -0.5
1,739,933 - RR42_RS28440 0.35 +0.1
1,739,933 - RR42_RS28440 0.35 +1.1
1,739,933 - RR42_RS28440 0.35 -0.3
1,739,956 + RR42_RS28440 0.37 +0.2
1,739,956 + RR42_RS28440 0.37 +1.0
1,739,957 - RR42_RS28440 0.37 -0.7
1,740,153 + RR42_RS28440 0.54 +0.9
1,740,395 + RR42_RS28440 0.74 +0.0
1,740,493 - RR42_RS28440 0.83 +0.0
1,740,695 + -1.8
1,740,695 + -0.7
1,740,695 + +0.9
1,741,056 - RR42_RS28445 0.23 -1.7
1,741,056 - RR42_RS28445 0.23 -0.4
1,741,056 - RR42_RS28445 0.23 -0.7
1,741,062 - RR42_RS28445 0.24 +2.9
1,741,320 - RR42_RS28445 0.50 -1.8
1,741,478 - RR42_RS28445 0.66 -0.7
1,741,726 + +0.3
1,741,796 + -0.3
1,741,796 + -0.4
1,741,905 - +1.1
1,741,905 - +0.1
1,741,994 + -0.3
1,741,995 - +0.3
1,742,231 + RR42_RS28450 0.27 +2.7
1,742,232 - RR42_RS28450 0.27 -0.4
1,742,232 - RR42_RS28450 0.27 -0.5
1,742,232 - RR42_RS28450 0.27 +0.7
1,742,385 - RR42_RS28450 0.38 -0.5
1,742,465 + RR42_RS28450 0.44 +0.5
1,742,507 + RR42_RS28450 0.47 -0.1
1,742,508 - RR42_RS28450 0.47 +0.3
1,742,562 - RR42_RS28450 0.51 +0.7
1,742,640 + RR42_RS28450 0.56 -1.0
1,742,640 + RR42_RS28450 0.56 -0.6
1,742,640 + RR42_RS28450 0.56 +0.1
1,742,640 + RR42_RS28450 0.56 +0.9
1,742,640 + RR42_RS28450 0.56 -0.1
1,742,641 - RR42_RS28450 0.57 -0.3
1,742,641 - RR42_RS28450 0.57 +0.0
1,742,641 - RR42_RS28450 0.57 +0.0
1,742,641 - RR42_RS28450 0.57 +0.7
1,742,641 - RR42_RS28450 0.57 -0.3
1,742,771 + RR42_RS28450 0.66 -0.6
1,742,771 + RR42_RS28450 0.66 -3.0
1,742,771 + RR42_RS28450 0.66 +0.0

Or see this region's nucleotide sequence