Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26710 and RR42_RS26715 are separated by 20 nucleotides RR42_RS26715 and RR42_RS26720 overlap by 97 nucleotides RR42_RS26720 and RR42_RS26725 overlap by 44 nucleotides RR42_RS26725 and RR42_RS26730 are separated by 7 nucleotides
RR42_RS26710: RR42_RS26710 - alkanesulfonate monooxygenase, at 1,349,720 to 1,350,829
_RS26710
RR42_RS26715: RR42_RS26715 - sulfonate ABC transporter substrate-binding protein, at 1,350,850 to 1,351,818
_RS26715
RR42_RS26720: RR42_RS26720 - hypothetical protein, at 1,351,722 to 1,351,940
_RS26720
RR42_RS26725: RR42_RS26725 - diguanylate cyclase, at 1,351,897 to 1,352,634
_RS26725
RR42_RS26730: RR42_RS26730 - Fe-S oxidoreductase, at 1,352,642 to 1,353,031
_RS26730
Position (kb)
1350
1351
1352 Strain fitness (log2 ratio)
-1
0
1 at 1349.903 kb on + strand, within RR42_RS26710 at 1349.904 kb on - strand, within RR42_RS26710 at 1349.904 kb on - strand, within RR42_RS26710 at 1350.176 kb on + strand, within RR42_RS26710 at 1350.176 kb on + strand, within RR42_RS26710 at 1350.176 kb on + strand, within RR42_RS26710 at 1350.176 kb on + strand, within RR42_RS26710 at 1350.176 kb on + strand, within RR42_RS26710 at 1350.177 kb on - strand, within RR42_RS26710 at 1350.177 kb on - strand, within RR42_RS26710 at 1350.177 kb on - strand, within RR42_RS26710 at 1350.180 kb on - strand, within RR42_RS26710 at 1350.296 kb on + strand, within RR42_RS26710 at 1350.297 kb on - strand, within RR42_RS26710 at 1350.297 kb on - strand, within RR42_RS26710 at 1350.335 kb on - strand, within RR42_RS26710 at 1350.668 kb on + strand, within RR42_RS26710 at 1350.671 kb on + strand, within RR42_RS26710 at 1350.672 kb on - strand, within RR42_RS26710 at 1350.672 kb on - strand, within RR42_RS26710 at 1350.672 kb on - strand, within RR42_RS26710 at 1350.734 kb on + strand at 1351.018 kb on + strand, within RR42_RS26715 at 1351.019 kb on - strand, within RR42_RS26715 at 1351.019 kb on - strand, within RR42_RS26715 at 1351.409 kb on - strand, within RR42_RS26715 at 1351.483 kb on + strand, within RR42_RS26715 at 1351.483 kb on + strand, within RR42_RS26715 at 1351.484 kb on - strand, within RR42_RS26715 at 1351.574 kb on - strand, within RR42_RS26715 at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.723 kb on + strand at 1351.970 kb on + strand at 1351.994 kb on + strand, within RR42_RS26725 at 1352.249 kb on - strand, within RR42_RS26725 at 1352.476 kb on + strand, within RR42_RS26725 at 1352.647 kb on + strand at 1352.742 kb on + strand, within RR42_RS26730
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,349,903 + RR42_RS26710 0.16 -0.1 1,349,904 - RR42_RS26710 0.17 +0.7 1,349,904 - RR42_RS26710 0.17 +0.1 1,350,176 + RR42_RS26710 0.41 -0.3 1,350,176 + RR42_RS26710 0.41 +0.5 1,350,176 + RR42_RS26710 0.41 +0.1 1,350,176 + RR42_RS26710 0.41 -0.2 1,350,176 + RR42_RS26710 0.41 +0.2 1,350,177 - RR42_RS26710 0.41 -0.9 1,350,177 - RR42_RS26710 0.41 -0.4 1,350,177 - RR42_RS26710 0.41 +0.6 1,350,180 - RR42_RS26710 0.41 -0.5 1,350,296 + RR42_RS26710 0.52 +0.8 1,350,297 - RR42_RS26710 0.52 -0.3 1,350,297 - RR42_RS26710 0.52 +0.5 1,350,335 - RR42_RS26710 0.55 +0.4 1,350,668 + RR42_RS26710 0.85 +0.0 1,350,671 + RR42_RS26710 0.86 +0.6 1,350,672 - RR42_RS26710 0.86 -0.7 1,350,672 - RR42_RS26710 0.86 +0.6 1,350,672 - RR42_RS26710 0.86 -0.3 1,350,734 + -1.1 1,351,018 + RR42_RS26715 0.17 -1.1 1,351,019 - RR42_RS26715 0.17 -1.3 1,351,019 - RR42_RS26715 0.17 +0.8 1,351,409 - RR42_RS26715 0.58 +0.1 1,351,483 + RR42_RS26715 0.65 -0.8 1,351,483 + RR42_RS26715 0.65 -0.9 1,351,484 - RR42_RS26715 0.65 -1.5 1,351,574 - RR42_RS26715 0.75 -1.5 1,351,723 + -0.7 1,351,723 + -1.1 1,351,723 + +0.8 1,351,723 + -0.8 1,351,970 + -0.7 1,351,994 + RR42_RS26725 0.13 -1.1 1,352,249 - RR42_RS26725 0.48 +0.5 1,352,476 + RR42_RS26725 0.78 +0.0 1,352,647 + -0.6 1,352,742 + RR42_RS26730 0.26 -0.5
Or see this region's nucleotide sequence