Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS26635 and RR42_RS26640 are separated by 123 nucleotides RR42_RS26640 and RR42_RS26645 are separated by 46 nucleotides
RR42_RS26635: RR42_RS26635 - 2-hydroxyacid dehydrogenase, at 1,328,791 to 1,330,191
_RS26635
RR42_RS26640: RR42_RS26640 - 2-keto-3-deoxy-L-rhamnonate aldolase, at 1,330,315 to 1,331,118
_RS26640
RR42_RS26645: RR42_RS26645 - succinate-semialdehyde dehydrogenase, at 1,331,165 to 1,332,649
_RS26645
Position (kb)
1330
1331
1332 Strain fitness (log2 ratio)
-1
0
1
2 at 1329.419 kb on + strand, within RR42_RS26635 at 1329.683 kb on + strand, within RR42_RS26635 at 1330.000 kb on - strand, within RR42_RS26635 at 1330.122 kb on - strand at 1330.122 kb on - strand at 1330.133 kb on + strand at 1330.319 kb on + strand at 1330.320 kb on - strand at 1330.320 kb on - strand at 1330.397 kb on + strand, within RR42_RS26640 at 1330.397 kb on + strand, within RR42_RS26640 at 1330.397 kb on + strand, within RR42_RS26640 at 1330.602 kb on + strand, within RR42_RS26640 at 1330.603 kb on - strand, within RR42_RS26640 at 1330.798 kb on + strand, within RR42_RS26640 at 1330.952 kb on - strand, within RR42_RS26640 at 1331.188 kb on + strand at 1331.190 kb on + strand at 1331.191 kb on - strand at 1331.205 kb on + strand at 1331.205 kb on + strand at 1331.205 kb on + strand at 1331.370 kb on + strand, within RR42_RS26645 at 1331.517 kb on + strand, within RR42_RS26645 at 1331.517 kb on + strand, within RR42_RS26645 at 1331.518 kb on - strand, within RR42_RS26645 at 1331.521 kb on - strand, within RR42_RS26645 at 1331.715 kb on + strand, within RR42_RS26645 at 1331.716 kb on - strand, within RR42_RS26645 at 1331.736 kb on - strand, within RR42_RS26645 at 1331.739 kb on + strand, within RR42_RS26645 at 1332.021 kb on - strand, within RR42_RS26645 at 1332.021 kb on - strand, within RR42_RS26645 at 1332.054 kb on + strand, within RR42_RS26645 at 1332.054 kb on + strand, within RR42_RS26645
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,329,419 + RR42_RS26635 0.45 -1.0 1,329,683 + RR42_RS26635 0.64 -0.0 1,330,000 - RR42_RS26635 0.86 -0.6 1,330,122 - -0.2 1,330,122 - -0.1 1,330,133 + -0.6 1,330,319 + -0.3 1,330,320 - +0.8 1,330,320 - +0.2 1,330,397 + RR42_RS26640 0.10 -0.0 1,330,397 + RR42_RS26640 0.10 +1.3 1,330,397 + RR42_RS26640 0.10 +1.9 1,330,602 + RR42_RS26640 0.36 -1.1 1,330,603 - RR42_RS26640 0.36 +0.1 1,330,798 + RR42_RS26640 0.60 +1.1 1,330,952 - RR42_RS26640 0.79 +1.2 1,331,188 + -0.0 1,331,190 + +0.5 1,331,191 - -1.0 1,331,205 + -0.0 1,331,205 + -0.4 1,331,205 + -0.9 1,331,370 + RR42_RS26645 0.14 -0.2 1,331,517 + RR42_RS26645 0.24 +0.5 1,331,517 + RR42_RS26645 0.24 -0.3 1,331,518 - RR42_RS26645 0.24 +0.4 1,331,521 - RR42_RS26645 0.24 -0.6 1,331,715 + RR42_RS26645 0.37 +0.7 1,331,716 - RR42_RS26645 0.37 +1.4 1,331,736 - RR42_RS26645 0.38 -0.6 1,331,739 + RR42_RS26645 0.39 -0.1 1,332,021 - RR42_RS26645 0.58 -0.0 1,332,021 - RR42_RS26645 0.58 +0.2 1,332,054 + RR42_RS26645 0.60 -0.9 1,332,054 + RR42_RS26645 0.60 -1.0
Or see this region's nucleotide sequence