Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25480

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25470 and RR42_RS25475 overlap by 4 nucleotidesRR42_RS25475 and RR42_RS25480 are separated by 11 nucleotidesRR42_RS25480 and RR42_RS25485 are separated by 16 nucleotidesRR42_RS25485 and RR42_RS25490 are separated by 30 nucleotides RR42_RS25470: RR42_RS25470 - alpha/beta hydrolase, at 1,061,322 to 1,062,113 _RS25470 RR42_RS25475: RR42_RS25475 - peptidase S15, at 1,062,110 to 1,063,024 _RS25475 RR42_RS25480: RR42_RS25480 - enoyl-CoA hydratase, at 1,063,036 to 1,063,845 _RS25480 RR42_RS25485: RR42_RS25485 - hypothetical protein, at 1,063,862 to 1,064,335 _RS25485 RR42_RS25490: RR42_RS25490 - ABC transporter substrate-binding protein, at 1,064,366 to 1,065,352 _RS25490 Position (kb) 1063 1064Strain fitness (log2 ratio) -2 -1 0 1at 1062.042 kb on + strandat 1062.047 kb on - strandat 1062.103 kb on - strandat 1062.113 kb on + strandat 1062.113 kb on + strandat 1062.145 kb on - strandat 1062.351 kb on + strand, within RR42_RS25475at 1062.351 kb on + strand, within RR42_RS25475at 1062.351 kb on + strand, within RR42_RS25475at 1062.351 kb on + strand, within RR42_RS25475at 1062.352 kb on - strand, within RR42_RS25475at 1062.457 kb on - strand, within RR42_RS25475at 1062.632 kb on + strand, within RR42_RS25475at 1062.684 kb on + strand, within RR42_RS25475at 1062.685 kb on - strand, within RR42_RS25475at 1062.742 kb on - strand, within RR42_RS25475at 1062.894 kb on + strand, within RR42_RS25475at 1062.894 kb on + strand, within RR42_RS25475at 1063.005 kb on + strandat 1063.005 kb on + strandat 1063.005 kb on + strandat 1063.006 kb on - strandat 1063.006 kb on - strandat 1063.402 kb on + strand, within RR42_RS25480at 1063.444 kb on - strand, within RR42_RS25480at 1063.515 kb on - strand, within RR42_RS25480at 1063.739 kb on + strand, within RR42_RS25480at 1063.740 kb on - strand, within RR42_RS25480at 1063.894 kb on + strandat 1064.066 kb on + strand, within RR42_RS25485at 1064.067 kb on - strand, within RR42_RS25485at 1064.074 kb on + strand, within RR42_RS25485at 1064.075 kb on - strand, within RR42_RS25485at 1064.082 kb on + strand, within RR42_RS25485at 1064.196 kb on + strand, within RR42_RS25485at 1064.196 kb on + strand, within RR42_RS25485at 1064.259 kb on + strand, within RR42_RS25485at 1064.259 kb on + strand, within RR42_RS25485at 1064.259 kb on + strand, within RR42_RS25485at 1064.259 kb on + strand, within RR42_RS25485at 1064.260 kb on - strand, within RR42_RS25485at 1064.260 kb on - strand, within RR42_RS25485at 1064.260 kb on - strand, within RR42_RS25485at 1064.278 kb on + strand, within RR42_RS25485at 1064.394 kb on - strandat 1064.428 kb on - strandat 1064.458 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,062,042 + -0.9
1,062,047 - -0.7
1,062,103 - +0.1
1,062,113 + -0.1
1,062,113 + -0.3
1,062,145 - -0.4
1,062,351 + RR42_RS25475 0.26 -0.6
1,062,351 + RR42_RS25475 0.26 -1.1
1,062,351 + RR42_RS25475 0.26 +0.2
1,062,351 + RR42_RS25475 0.26 +0.5
1,062,352 - RR42_RS25475 0.26 +0.5
1,062,457 - RR42_RS25475 0.38 +0.2
1,062,632 + RR42_RS25475 0.57 -0.4
1,062,684 + RR42_RS25475 0.63 -0.6
1,062,685 - RR42_RS25475 0.63 -0.2
1,062,742 - RR42_RS25475 0.69 +0.6
1,062,894 + RR42_RS25475 0.86 -2.2
1,062,894 + RR42_RS25475 0.86 -0.7
1,063,005 + -0.4
1,063,005 + -0.2
1,063,005 + -0.5
1,063,006 - -0.2
1,063,006 - -0.2
1,063,402 + RR42_RS25480 0.45 +0.2
1,063,444 - RR42_RS25480 0.50 -0.7
1,063,515 - RR42_RS25480 0.59 +0.4
1,063,739 + RR42_RS25480 0.87 +0.1
1,063,740 - RR42_RS25480 0.87 -0.4
1,063,894 + +0.2
1,064,066 + RR42_RS25485 0.43 -1.4
1,064,067 - RR42_RS25485 0.43 -0.5
1,064,074 + RR42_RS25485 0.45 +0.6
1,064,075 - RR42_RS25485 0.45 +0.2
1,064,082 + RR42_RS25485 0.46 +0.3
1,064,196 + RR42_RS25485 0.70 -0.8
1,064,196 + RR42_RS25485 0.70 +0.1
1,064,259 + RR42_RS25485 0.84 +0.0
1,064,259 + RR42_RS25485 0.84 +0.5
1,064,259 + RR42_RS25485 0.84 +0.4
1,064,259 + RR42_RS25485 0.84 -0.7
1,064,260 - RR42_RS25485 0.84 +0.4
1,064,260 - RR42_RS25485 0.84 -0.1
1,064,260 - RR42_RS25485 0.84 -0.5
1,064,278 + RR42_RS25485 0.88 -0.6
1,064,394 - -0.5
1,064,428 - -0.7
1,064,458 - -0.2

Or see this region's nucleotide sequence