Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25095 and RR42_RS25100 are separated by 26 nucleotides RR42_RS25100 and RR42_RS25105 are separated by 24 nucleotides RR42_RS25105 and RR42_RS25110 are separated by 143 nucleotides
RR42_RS25095: RR42_RS25095 - citryl-CoA lyase, at 968,351 to 969,241
_RS25095
RR42_RS25100: RR42_RS25100 - dehydratase, at 969,268 to 969,729
_RS25100
RR42_RS25105: RR42_RS25105 - acyl-CoA dehydrogenase, at 969,754 to 970,920
_RS25105
RR42_RS25110: RR42_RS25110 - nitroreductase, at 971,064 to 971,747
_RS25110
Position (kb) 969
970
971 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 968.771 kb on + strand, within RR42_RS25095 at 968.772 kb on - strand, within RR42_RS25095 at 968.772 kb on - strand, within RR42_RS25095 at 968.891 kb on + strand, within RR42_RS25095 at 968.952 kb on + strand, within RR42_RS25095 at 969.071 kb on + strand, within RR42_RS25095 at 969.071 kb on + strand, within RR42_RS25095 at 969.072 kb on - strand, within RR42_RS25095 at 969.072 kb on - strand, within RR42_RS25095 at 969.072 kb on - strand at 969.102 kb on - strand, within RR42_RS25095 at 969.253 kb on + strand at 969.254 kb on - strand at 969.254 kb on - strand at 969.254 kb on - strand at 969.373 kb on + strand, within RR42_RS25100 at 969.374 kb on - strand, within RR42_RS25100 at 969.418 kb on - strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.760 kb on + strand at 969.760 kb on + strand at 970.321 kb on + strand, within RR42_RS25105 at 970.322 kb on - strand, within RR42_RS25105 at 970.342 kb on + strand, within RR42_RS25105 at 970.343 kb on - strand, within RR42_RS25105 at 970.343 kb on - strand, within RR42_RS25105 at 970.465 kb on + strand, within RR42_RS25105 at 970.466 kb on - strand, within RR42_RS25105 at 970.466 kb on - strand, within RR42_RS25105 at 970.466 kb on - strand, within RR42_RS25105 at 970.466 kb on - strand, within RR42_RS25105 at 970.869 kb on + strand at 970.869 kb on + strand at 971.096 kb on - strand at 971.133 kb on + strand, within RR42_RS25110 at 971.230 kb on + strand, within RR42_RS25110 at 971.234 kb on + strand, within RR42_RS25110 at 971.235 kb on - strand, within RR42_RS25110 at 971.343 kb on + strand, within RR42_RS25110 at 971.379 kb on + strand, within RR42_RS25110 at 971.380 kb on - strand, within RR42_RS25110 at 971.502 kb on + strand, within RR42_RS25110 at 971.502 kb on + strand, within RR42_RS25110 at 971.503 kb on - strand, within RR42_RS25110 at 971.503 kb on - strand, within RR42_RS25110 at 971.889 kb on + strand at 971.911 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 968,771 + RR42_RS25095 0.47 +0.5 968,772 - RR42_RS25095 0.47 +0.0 968,772 - RR42_RS25095 0.47 -0.1 968,891 + RR42_RS25095 0.61 -0.3 968,952 + RR42_RS25095 0.67 -3.0 969,071 + RR42_RS25095 0.81 -0.2 969,071 + RR42_RS25095 0.81 +0.2 969,072 - RR42_RS25095 0.81 -0.6 969,072 - RR42_RS25095 0.81 -0.1 969,072 - -0.3 969,102 - RR42_RS25095 0.84 +0.5 969,253 + +1.1 969,254 - -0.3 969,254 - -1.4 969,254 - -0.4 969,373 + RR42_RS25100 0.23 +0.1 969,374 - RR42_RS25100 0.23 -0.3 969,418 - RR42_RS25100 0.32 +0.1 969,484 + RR42_RS25100 0.47 +0.2 969,484 + RR42_RS25100 0.47 -0.6 969,484 + RR42_RS25100 0.47 +0.1 969,484 + RR42_RS25100 0.47 -0.1 969,760 + -0.7 969,760 + +1.0 970,321 + RR42_RS25105 0.49 -0.4 970,322 - RR42_RS25105 0.49 -1.3 970,342 + RR42_RS25105 0.50 -0.2 970,343 - RR42_RS25105 0.50 -1.4 970,343 - RR42_RS25105 0.50 -0.0 970,465 + RR42_RS25105 0.61 +0.4 970,466 - RR42_RS25105 0.61 -1.1 970,466 - RR42_RS25105 0.61 -1.1 970,466 - RR42_RS25105 0.61 +0.0 970,466 - RR42_RS25105 0.61 +0.0 970,869 + -2.2 970,869 + +0.3 971,096 - -0.1 971,133 + RR42_RS25110 0.10 +0.5 971,230 + RR42_RS25110 0.24 -0.1 971,234 + RR42_RS25110 0.25 +0.1 971,235 - RR42_RS25110 0.25 +0.7 971,343 + RR42_RS25110 0.41 +1.3 971,379 + RR42_RS25110 0.46 -0.1 971,380 - RR42_RS25110 0.46 +0.1 971,502 + RR42_RS25110 0.64 +0.8 971,502 + RR42_RS25110 0.64 -0.0 971,503 - RR42_RS25110 0.64 -0.7 971,503 - RR42_RS25110 0.64 -0.3 971,889 + +0.2 971,911 + -0.4
Or see this region's nucleotide sequence