Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25085 and RR42_RS25090 are separated by 156 nucleotides RR42_RS25090 and RR42_RS25095 are separated by 166 nucleotides RR42_RS25095 and RR42_RS25100 are separated by 26 nucleotides RR42_RS25100 and RR42_RS25105 are separated by 24 nucleotides
RR42_RS25085: RR42_RS25085 - transcriptional regulator, at 966,622 to 967,557
_RS25085
RR42_RS25090: RR42_RS25090 - hypothetical protein, at 967,714 to 968,184
_RS25090
RR42_RS25095: RR42_RS25095 - citryl-CoA lyase, at 968,351 to 969,241
_RS25095
RR42_RS25100: RR42_RS25100 - dehydratase, at 969,268 to 969,729
_RS25100
RR42_RS25105: RR42_RS25105 - acyl-CoA dehydrogenase, at 969,754 to 970,920
_RS25105
Position (kb) 968
969
970 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 967.739 kb on - strand at 967.831 kb on + strand, within RR42_RS25090 at 967.831 kb on + strand, within RR42_RS25090 at 967.831 kb on + strand, within RR42_RS25090 at 967.831 kb on + strand, within RR42_RS25090 at 967.832 kb on - strand, within RR42_RS25090 at 967.832 kb on - strand, within RR42_RS25090 at 967.832 kb on - strand, within RR42_RS25090 at 967.864 kb on + strand, within RR42_RS25090 at 967.864 kb on + strand, within RR42_RS25090 at 968.111 kb on - strand, within RR42_RS25090 at 968.239 kb on + strand at 968.239 kb on + strand at 968.239 kb on + strand at 968.240 kb on - strand at 968.248 kb on - strand at 968.267 kb on - strand at 968.267 kb on - strand at 968.337 kb on - strand at 968.358 kb on + strand at 968.358 kb on + strand at 968.585 kb on + strand, within RR42_RS25095 at 968.591 kb on + strand, within RR42_RS25095 at 968.672 kb on - strand, within RR42_RS25095 at 968.771 kb on + strand, within RR42_RS25095 at 968.772 kb on - strand, within RR42_RS25095 at 968.772 kb on - strand, within RR42_RS25095 at 968.891 kb on + strand, within RR42_RS25095 at 968.952 kb on + strand, within RR42_RS25095 at 969.071 kb on + strand, within RR42_RS25095 at 969.071 kb on + strand, within RR42_RS25095 at 969.072 kb on - strand, within RR42_RS25095 at 969.072 kb on - strand, within RR42_RS25095 at 969.072 kb on - strand at 969.102 kb on - strand, within RR42_RS25095 at 969.253 kb on + strand at 969.254 kb on - strand at 969.254 kb on - strand at 969.254 kb on - strand at 969.373 kb on + strand, within RR42_RS25100 at 969.374 kb on - strand, within RR42_RS25100 at 969.418 kb on - strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.484 kb on + strand, within RR42_RS25100 at 969.760 kb on + strand at 969.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 967,739 - -0.0 967,831 + RR42_RS25090 0.25 +0.1 967,831 + RR42_RS25090 0.25 -1.6 967,831 + RR42_RS25090 0.25 -0.7 967,831 + RR42_RS25090 0.25 -1.8 967,832 - RR42_RS25090 0.25 -0.2 967,832 - RR42_RS25090 0.25 +0.5 967,832 - RR42_RS25090 0.25 -0.2 967,864 + RR42_RS25090 0.32 +0.1 967,864 + RR42_RS25090 0.32 -0.2 968,111 - RR42_RS25090 0.84 +0.0 968,239 + -1.6 968,239 + +1.4 968,239 + +0.2 968,240 - -0.5 968,248 - +0.7 968,267 - +0.3 968,267 - +0.1 968,337 - +0.5 968,358 + -1.3 968,358 + +1.0 968,585 + RR42_RS25095 0.26 +0.7 968,591 + RR42_RS25095 0.27 +0.7 968,672 - RR42_RS25095 0.36 -1.6 968,771 + RR42_RS25095 0.47 +0.5 968,772 - RR42_RS25095 0.47 +0.0 968,772 - RR42_RS25095 0.47 -0.1 968,891 + RR42_RS25095 0.61 -0.3 968,952 + RR42_RS25095 0.67 -3.0 969,071 + RR42_RS25095 0.81 -0.2 969,071 + RR42_RS25095 0.81 +0.2 969,072 - RR42_RS25095 0.81 -0.6 969,072 - RR42_RS25095 0.81 -0.1 969,072 - -0.3 969,102 - RR42_RS25095 0.84 +0.5 969,253 + +1.1 969,254 - -0.3 969,254 - -1.4 969,254 - -0.4 969,373 + RR42_RS25100 0.23 +0.1 969,374 - RR42_RS25100 0.23 -0.3 969,418 - RR42_RS25100 0.32 +0.1 969,484 + RR42_RS25100 0.47 +0.2 969,484 + RR42_RS25100 0.47 -0.6 969,484 + RR42_RS25100 0.47 +0.1 969,484 + RR42_RS25100 0.47 -0.1 969,760 + -0.7 969,760 + +1.0
Or see this region's nucleotide sequence