Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS25020 and RR42_RS25025 are separated by 21 nucleotides RR42_RS25025 and RR42_RS25030 are separated by 53 nucleotides RR42_RS25030 and RR42_RS25035 overlap by 4 nucleotides RR42_RS25035 and RR42_RS25040 are separated by 6 nucleotides
RR42_RS25020: RR42_RS25020 - amino acid ABC transporter ATPase, at 951,371 to 952,129
_RS25020
RR42_RS25025: RR42_RS25025 - sugar ABC transporter ATPase, at 952,151 to 952,921
_RS25025
RR42_RS25030: RR42_RS25030 - ABC transporter permease, at 952,975 to 953,820
_RS25030
RR42_RS25035: RR42_RS25035 - ABC transporter permease, at 953,817 to 954,710
_RS25035
RR42_RS25040: RR42_RS25040 - amino acid ABC transporter substrate-binding protein, at 954,717 to 955,853
_RS25040
Position (kb) 952
953
954 Strain fitness (log2 ratio) -1
0
1 at 952.164 kb on + strand at 952.165 kb on - strand at 952.242 kb on + strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.243 kb on - strand, within RR42_RS25025 at 952.248 kb on + strand, within RR42_RS25025 at 952.249 kb on - strand, within RR42_RS25025 at 952.430 kb on - strand, within RR42_RS25025 at 953.036 kb on + strand at 953.307 kb on - strand, within RR42_RS25030 at 953.682 kb on - strand, within RR42_RS25030 at 953.769 kb on - strand at 953.769 kb on - strand at 953.810 kb on + strand at 953.811 kb on - strand at 953.811 kb on - strand at 953.820 kb on + strand at 953.933 kb on + strand, within RR42_RS25035 at 953.934 kb on - strand, within RR42_RS25035 at 953.934 kb on - strand, within RR42_RS25035 at 953.935 kb on + strand, within RR42_RS25035 at 953.935 kb on + strand, within RR42_RS25035 at 953.935 kb on + strand, within RR42_RS25035 at 953.935 kb on + strand, within RR42_RS25035 at 953.936 kb on - strand, within RR42_RS25035 at 953.936 kb on - strand, within RR42_RS25035 at 953.936 kb on - strand, within RR42_RS25035 at 953.941 kb on + strand, within RR42_RS25035 at 954.179 kb on + strand, within RR42_RS25035 at 954.319 kb on + strand, within RR42_RS25035 at 954.319 kb on + strand, within RR42_RS25035 at 954.320 kb on - strand, within RR42_RS25035 at 954.428 kb on - strand, within RR42_RS25035 at 954.474 kb on + strand, within RR42_RS25035 at 954.495 kb on + strand, within RR42_RS25035 at 954.772 kb on + strand at 954.804 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 952,164 + +0.5 952,165 - -0.5 952,242 + RR42_RS25025 0.12 -0.0 952,243 - RR42_RS25025 0.12 +0.0 952,243 - RR42_RS25025 0.12 +0.5 952,243 - RR42_RS25025 0.12 +0.7 952,248 + RR42_RS25025 0.13 +0.8 952,249 - RR42_RS25025 0.13 +0.3 952,430 - RR42_RS25025 0.36 -0.1 953,036 + -0.7 953,307 - RR42_RS25030 0.39 +0.4 953,682 - RR42_RS25030 0.84 +0.9 953,769 - +0.4 953,769 - -0.3 953,810 + +0.4 953,811 - +1.3 953,811 - -0.0 953,820 + -0.1 953,933 + RR42_RS25035 0.13 +0.9 953,934 - RR42_RS25035 0.13 -0.2 953,934 - RR42_RS25035 0.13 +0.1 953,935 + RR42_RS25035 0.13 +0.7 953,935 + RR42_RS25035 0.13 -0.4 953,935 + RR42_RS25035 0.13 +1.0 953,935 + RR42_RS25035 0.13 -0.1 953,936 - RR42_RS25035 0.13 -1.2 953,936 - RR42_RS25035 0.13 +0.1 953,936 - RR42_RS25035 0.13 -0.2 953,941 + RR42_RS25035 0.14 -0.3 954,179 + RR42_RS25035 0.40 +0.1 954,319 + RR42_RS25035 0.56 -0.6 954,319 + RR42_RS25035 0.56 +0.1 954,320 - RR42_RS25035 0.56 +0.1 954,428 - RR42_RS25035 0.68 +0.8 954,474 + RR42_RS25035 0.73 +0.1 954,495 + RR42_RS25035 0.76 -0.1 954,772 + +0.0 954,804 - -0.7
Or see this region's nucleotide sequence