Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24980 and RR42_RS24985 overlap by 2 nucleotides RR42_RS24985 and RR42_RS24990 overlap by 4 nucleotides RR42_RS24990 and RR42_RS24995 are separated by 384 nucleotides
RR42_RS24980: RR42_RS24980 - 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, at 942,641 to 943,534
_RS24980
RR42_RS24985: RR42_RS24985 - hypothetical protein, at 943,533 to 943,787
_RS24985
RR42_RS24990: RR42_RS24990 - acyl-CoA dehydrogenase, at 943,784 to 944,950
_RS24990
RR42_RS24995: RR42_RS24995 - hypothetical protein, at 945,335 to 946,114
_RS24995
Position (kb) 943
944
945 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 943.081 kb on - strand, within RR42_RS24980 at 943.452 kb on + strand at 943.453 kb on - strand at 943.453 kb on - strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.521 kb on + strand at 943.522 kb on - strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.600 kb on - strand, within RR42_RS24985 at 943.600 kb on - strand, within RR42_RS24985 at 943.648 kb on + strand, within RR42_RS24985 at 943.833 kb on + strand at 943.866 kb on - strand at 943.922 kb on - strand, within RR42_RS24990 at 943.961 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.225 kb on + strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.495 kb on + strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.559 kb on - strand, within RR42_RS24990 at 944.585 kb on + strand, within RR42_RS24990 at 944.585 kb on + strand, within RR42_RS24990 at 944.585 kb on + strand, within RR42_RS24990 at 944.586 kb on - strand, within RR42_RS24990 at 944.586 kb on - strand, within RR42_RS24990 at 944.586 kb on - strand, within RR42_RS24990 at 944.586 kb on - strand, within RR42_RS24990 at 944.757 kb on - strand, within RR42_RS24990 at 944.757 kb on - strand, within RR42_RS24990 at 944.757 kb on - strand, within RR42_RS24990 at 944.757 kb on - strand, within RR42_RS24990 at 944.804 kb on + strand, within RR42_RS24990 at 944.834 kb on + strand at 944.835 kb on - strand at 944.835 kb on - strand at 944.903 kb on + strand at 944.903 kb on + strand at 944.903 kb on + strand at 944.903 kb on + strand at 944.904 kb on - strand at 945.267 kb on - strand at 945.276 kb on + strand at 945.276 kb on + strand at 945.285 kb on + strand at 945.323 kb on - strand at 945.340 kb on + strand at 945.341 kb on - strand at 945.349 kb on + strand at 945.349 kb on + strand at 945.359 kb on - strand at 945.453 kb on + strand, within RR42_RS24995 at 945.454 kb on - strand, within RR42_RS24995 at 945.546 kb on + strand, within RR42_RS24995 at 945.546 kb on + strand, within RR42_RS24995 at 945.546 kb on + strand, within RR42_RS24995 at 945.547 kb on - strand, within RR42_RS24995 at 945.547 kb on - strand, within RR42_RS24995 at 945.588 kb on + strand, within RR42_RS24995 at 945.588 kb on + strand, within RR42_RS24995 at 945.589 kb on - strand, within RR42_RS24995 at 945.596 kb on + strand, within RR42_RS24995 at 945.596 kb on + strand, within RR42_RS24995 at 945.634 kb on - strand, within RR42_RS24995 at 945.710 kb on - strand, within RR42_RS24995 at 945.821 kb on + strand, within RR42_RS24995 at 945.822 kb on - strand, within RR42_RS24995 at 945.822 kb on - strand, within RR42_RS24995 at 945.876 kb on - strand, within RR42_RS24995 at 945.876 kb on - strand, within RR42_RS24995 at 945.876 kb on - strand, within RR42_RS24995 at 945.876 kb on - strand, within RR42_RS24995 at 945.936 kb on - strand, within RR42_RS24995 at 945.944 kb on + strand, within RR42_RS24995 at 945.945 kb on - strand, within RR42_RS24995 at 945.945 kb on - strand, within RR42_RS24995
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 943,081 - RR42_RS24980 0.49 -0.9 943,452 + -0.5 943,453 - +0.8 943,453 - -0.9 943,476 + +1.1 943,476 + -1.4 943,476 + +0.3 943,476 + -1.4 943,521 + -0.3 943,522 - +0.3 943,546 + +0.7 943,546 + -0.6 943,546 + -0.5 943,600 - RR42_RS24985 0.26 -0.6 943,600 - RR42_RS24985 0.26 +0.4 943,648 + RR42_RS24985 0.45 -0.5 943,833 + -1.9 943,866 - +0.0 943,922 - RR42_RS24990 0.12 -0.9 943,961 + RR42_RS24990 0.15 +0.4 944,057 + RR42_RS24990 0.23 -1.3 944,057 + RR42_RS24990 0.23 +0.7 944,058 - RR42_RS24990 0.23 +0.5 944,058 - RR42_RS24990 0.23 +0.3 944,058 - RR42_RS24990 0.23 +0.2 944,225 + RR42_RS24990 0.38 +0.4 944,226 - RR42_RS24990 0.38 +0.0 944,226 - RR42_RS24990 0.38 -0.3 944,495 + RR42_RS24990 0.61 +0.2 944,496 - RR42_RS24990 0.61 -0.0 944,496 - RR42_RS24990 0.61 +0.0 944,496 - RR42_RS24990 0.61 -0.4 944,559 - RR42_RS24990 0.66 -2.0 944,585 + RR42_RS24990 0.69 +0.0 944,585 + RR42_RS24990 0.69 -0.3 944,585 + RR42_RS24990 0.69 +0.4 944,586 - RR42_RS24990 0.69 +0.2 944,586 - RR42_RS24990 0.69 +0.2 944,586 - RR42_RS24990 0.69 -0.6 944,586 - RR42_RS24990 0.69 +0.1 944,757 - RR42_RS24990 0.83 -0.5 944,757 - RR42_RS24990 0.83 +0.2 944,757 - RR42_RS24990 0.83 -0.0 944,757 - RR42_RS24990 0.83 -0.0 944,804 + RR42_RS24990 0.87 -0.6 944,834 + -1.6 944,835 - -1.9 944,835 - +3.0 944,903 + -1.0 944,903 + +0.2 944,903 + +0.4 944,903 + -0.0 944,904 - -0.6 945,267 - -0.2 945,276 + -0.7 945,276 + -1.1 945,285 + -0.6 945,323 - +0.6 945,340 + -0.9 945,341 - +0.2 945,349 + -1.3 945,349 + +1.4 945,359 - -0.2 945,453 + RR42_RS24995 0.15 +0.3 945,454 - RR42_RS24995 0.15 +1.4 945,546 + RR42_RS24995 0.27 -0.2 945,546 + RR42_RS24995 0.27 +1.2 945,546 + RR42_RS24995 0.27 +0.3 945,547 - RR42_RS24995 0.27 -0.8 945,547 - RR42_RS24995 0.27 +0.8 945,588 + RR42_RS24995 0.32 -2.0 945,588 + RR42_RS24995 0.32 +0.1 945,589 - RR42_RS24995 0.33 -0.1 945,596 + RR42_RS24995 0.33 +0.5 945,596 + RR42_RS24995 0.33 +0.3 945,634 - RR42_RS24995 0.38 -0.5 945,710 - RR42_RS24995 0.48 +0.5 945,821 + RR42_RS24995 0.62 -0.2 945,822 - RR42_RS24995 0.62 -1.1 945,822 - RR42_RS24995 0.62 +0.3 945,876 - RR42_RS24995 0.69 -0.3 945,876 - RR42_RS24995 0.69 +0.7 945,876 - RR42_RS24995 0.69 +1.7 945,876 - RR42_RS24995 0.69 +1.7 945,936 - RR42_RS24995 0.77 +0.0 945,944 + RR42_RS24995 0.78 +0.4 945,945 - RR42_RS24995 0.78 +1.0 945,945 - RR42_RS24995 0.78 -0.4
Or see this region's nucleotide sequence