Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24975 and RR42_RS24980 are separated by 23 nucleotides RR42_RS24980 and RR42_RS24985 overlap by 2 nucleotides RR42_RS24985 and RR42_RS24990 overlap by 4 nucleotides
RR42_RS24975: RR42_RS24975 - acetyl-CoA hydrolase, at 941,301 to 942,617
_RS24975
RR42_RS24980: RR42_RS24980 - 3-alpha,7-alpha, 12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, at 942,641 to 943,534
_RS24980
RR42_RS24985: RR42_RS24985 - hypothetical protein, at 943,533 to 943,787
_RS24985
RR42_RS24990: RR42_RS24990 - acyl-CoA dehydrogenase, at 943,784 to 944,950
_RS24990
Position (kb) 942
943
944 Strain fitness (log2 ratio) -2
-1
0
1
2 at 941.742 kb on - strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.743 kb on + strand, within RR42_RS24975 at 941.744 kb on - strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.133 kb on + strand, within RR42_RS24975 at 942.134 kb on - strand, within RR42_RS24975 at 942.202 kb on + strand, within RR42_RS24975 at 942.203 kb on - strand, within RR42_RS24975 at 942.226 kb on + strand, within RR42_RS24975 at 942.264 kb on + strand, within RR42_RS24975 at 942.265 kb on - strand, within RR42_RS24975 at 942.294 kb on + strand, within RR42_RS24975 at 942.328 kb on - strand, within RR42_RS24975 at 943.081 kb on - strand, within RR42_RS24980 at 943.452 kb on + strand at 943.453 kb on - strand at 943.453 kb on - strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.476 kb on + strand at 943.521 kb on + strand at 943.522 kb on - strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.546 kb on + strand at 943.600 kb on - strand, within RR42_RS24985 at 943.600 kb on - strand, within RR42_RS24985 at 943.648 kb on + strand, within RR42_RS24985 at 943.833 kb on + strand at 943.866 kb on - strand at 943.922 kb on - strand, within RR42_RS24990 at 943.961 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.057 kb on + strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.058 kb on - strand, within RR42_RS24990 at 944.225 kb on + strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.226 kb on - strand, within RR42_RS24990 at 944.495 kb on + strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990 at 944.496 kb on - strand, within RR42_RS24990
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 941,742 - RR42_RS24975 0.33 -0.2 941,743 + RR42_RS24975 0.34 -0.5 941,743 + RR42_RS24975 0.34 +0.4 941,743 + RR42_RS24975 0.34 -0.4 941,743 + RR42_RS24975 0.34 +0.1 941,743 + RR42_RS24975 0.34 -1.8 941,743 + RR42_RS24975 0.34 +0.4 941,744 - RR42_RS24975 0.34 +0.4 942,133 + RR42_RS24975 0.63 +0.2 942,133 + RR42_RS24975 0.63 +1.0 942,133 + RR42_RS24975 0.63 +2.7 942,134 - RR42_RS24975 0.63 -0.3 942,202 + RR42_RS24975 0.68 -0.8 942,203 - RR42_RS24975 0.68 -1.0 942,226 + RR42_RS24975 0.70 +0.4 942,264 + RR42_RS24975 0.73 -0.7 942,265 - RR42_RS24975 0.73 -0.1 942,294 + RR42_RS24975 0.75 +0.3 942,328 - RR42_RS24975 0.78 +0.0 943,081 - RR42_RS24980 0.49 -0.9 943,452 + -0.5 943,453 - +0.8 943,453 - -0.9 943,476 + +1.1 943,476 + -1.4 943,476 + +0.3 943,476 + -1.4 943,521 + -0.3 943,522 - +0.3 943,546 + +0.7 943,546 + -0.6 943,546 + -0.5 943,600 - RR42_RS24985 0.26 -0.6 943,600 - RR42_RS24985 0.26 +0.4 943,648 + RR42_RS24985 0.45 -0.5 943,833 + -1.9 943,866 - +0.0 943,922 - RR42_RS24990 0.12 -0.9 943,961 + RR42_RS24990 0.15 +0.4 944,057 + RR42_RS24990 0.23 -1.3 944,057 + RR42_RS24990 0.23 +0.7 944,058 - RR42_RS24990 0.23 +0.5 944,058 - RR42_RS24990 0.23 +0.3 944,058 - RR42_RS24990 0.23 +0.2 944,225 + RR42_RS24990 0.38 +0.4 944,226 - RR42_RS24990 0.38 +0.0 944,226 - RR42_RS24990 0.38 -0.3 944,495 + RR42_RS24990 0.61 +0.2 944,496 - RR42_RS24990 0.61 -0.0 944,496 - RR42_RS24990 0.61 +0.0 944,496 - RR42_RS24990 0.61 -0.4
Or see this region's nucleotide sequence