Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24790 and RR42_RS24795 are separated by 174 nucleotides RR42_RS24795 and RR42_RS24800 are separated by 28 nucleotides RR42_RS24800 and RR42_RS24805 are separated by 10 nucleotides
RR42_RS24790: RR42_RS24790 - LysR family transcriptional regulator, at 900,148 to 901,089
_RS24790
RR42_RS24795: RR42_RS24795 - butyryl-CoA dehydrogenase, at 901,264 to 902,412
_RS24795
RR42_RS24800: RR42_RS24800 - dehydratase, at 902,441 to 902,956
_RS24800
RR42_RS24805: RR42_RS24805 - thiolase, at 902,967 to 904,181
_RS24805
Position (kb) 901
902
903 Strain fitness (log2 ratio) -2
-1
0
1 at 900.429 kb on - strand, within RR42_RS24790 at 900.914 kb on + strand, within RR42_RS24790 at 901.204 kb on - strand at 901.226 kb on + strand at 901.226 kb on + strand at 901.348 kb on + strand at 901.349 kb on - strand at 901.349 kb on - strand at 901.384 kb on + strand, within RR42_RS24795 at 901.385 kb on - strand, within RR42_RS24795 at 901.394 kb on - strand, within RR42_RS24795 at 901.594 kb on + strand, within RR42_RS24795 at 901.595 kb on - strand, within RR42_RS24795 at 902.018 kb on - strand, within RR42_RS24795 at 902.323 kb on + strand at 902.324 kb on - strand at 902.434 kb on + strand at 902.435 kb on - strand at 902.562 kb on - strand, within RR42_RS24800 at 902.562 kb on - strand, within RR42_RS24800 at 902.609 kb on + strand, within RR42_RS24800 at 902.660 kb on + strand, within RR42_RS24800 at 902.661 kb on - strand, within RR42_RS24800 at 902.711 kb on + strand, within RR42_RS24800 at 902.712 kb on - strand, within RR42_RS24800 at 902.712 kb on - strand, within RR42_RS24800 at 902.747 kb on + strand, within RR42_RS24800 at 902.747 kb on + strand, within RR42_RS24800 at 902.773 kb on - strand, within RR42_RS24800 at 903.013 kb on - strand at 903.013 kb on - strand at 903.405 kb on + strand, within RR42_RS24805
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 900,429 - RR42_RS24790 0.30 -0.1 900,914 + RR42_RS24790 0.81 -0.3 901,204 - -0.6 901,226 + -0.6 901,226 + -0.3 901,348 + -0.2 901,349 - -0.4 901,349 - -1.4 901,384 + RR42_RS24795 0.10 +0.2 901,385 - RR42_RS24795 0.11 -0.3 901,394 - RR42_RS24795 0.11 -0.9 901,594 + RR42_RS24795 0.29 -0.2 901,595 - RR42_RS24795 0.29 +0.1 902,018 - RR42_RS24795 0.66 -1.3 902,323 + -0.7 902,324 - -0.0 902,434 + +0.1 902,435 - -0.2 902,562 - RR42_RS24800 0.23 +0.4 902,562 - RR42_RS24800 0.23 -1.2 902,609 + RR42_RS24800 0.33 -1.9 902,660 + RR42_RS24800 0.42 +0.6 902,661 - RR42_RS24800 0.43 +0.5 902,711 + RR42_RS24800 0.52 +0.5 902,712 - RR42_RS24800 0.53 -1.6 902,712 - RR42_RS24800 0.53 -0.4 902,747 + RR42_RS24800 0.59 +0.6 902,747 + RR42_RS24800 0.59 +0.3 902,773 - RR42_RS24800 0.64 +0.7 903,013 - -0.6 903,013 - -0.7 903,405 + RR42_RS24805 0.36 -0.5
Or see this region's nucleotide sequence