Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS24175 and RR42_RS24180 are separated by 29 nucleotides
RR42_RS24175: RR42_RS24175 - isopropylmalate isomerase, at 749,287 to 750,696
_RS24175
RR42_RS24180: RR42_RS24180 - 3-isopropylmalate dehydratase, at 750,726 to 751,376
_RS24180
Position (kb) 750
751
752 Strain fitness (log2 ratio) -2
-1
0
1
2 at 750.010 kb on + strand, within RR42_RS24175 at 750.044 kb on + strand, within RR42_RS24175 at 750.045 kb on - strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.259 kb on + strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.260 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.340 kb on - strand, within RR42_RS24175 at 750.586 kb on - strand at 750.586 kb on - strand at 750.659 kb on + strand at 750.659 kb on + strand at 750.660 kb on - strand at 750.660 kb on - strand at 750.702 kb on + strand at 750.703 kb on - strand at 750.706 kb on + strand at 750.706 kb on + strand at 750.707 kb on - strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.742 kb on + strand at 750.931 kb on + strand, within RR42_RS24180 at 751.087 kb on - strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.167 kb on + strand, within RR42_RS24180 at 751.168 kb on - strand, within RR42_RS24180 at 751.225 kb on - strand, within RR42_RS24180 at 751.254 kb on + strand, within RR42_RS24180 at 751.255 kb on - strand, within RR42_RS24180 at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.379 kb on + strand at 751.380 kb on - strand at 751.457 kb on + strand at 751.458 kb on - strand at 751.472 kb on + strand at 751.499 kb on + strand at 751.521 kb on - strand at 751.557 kb on + strand at 751.558 kb on - strand at 751.601 kb on + strand at 751.617 kb on + strand at 751.618 kb on - strand at 751.630 kb on + strand at 751.630 kb on + strand at 751.669 kb on + strand at 751.669 kb on + strand at 751.692 kb on + strand at 751.692 kb on + strand at 751.734 kb on - strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.771 kb on - strand at 751.771 kb on - strand at 751.953 kb on + strand at 751.953 kb on + strand at 751.954 kb on - strand at 751.988 kb on - strand at 751.988 kb on - strand at 752.019 kb on + strand at 752.019 kb on + strand at 752.020 kb on - strand at 752.020 kb on - strand at 752.150 kb on - strand at 752.179 kb on - strand at 752.216 kb on + strand at 752.217 kb on - strand at 752.228 kb on + strand at 752.282 kb on + strand at 752.282 kb on + strand at 752.311 kb on + strand at 752.311 kb on + strand at 752.312 kb on - strand at 752.359 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 750,010 + RR42_RS24175 0.51 +0.0 750,044 + RR42_RS24175 0.54 -0.6 750,045 - RR42_RS24175 0.54 +0.6 750,259 + RR42_RS24175 0.69 +0.1 750,259 + RR42_RS24175 0.69 -0.1 750,259 + RR42_RS24175 0.69 -0.1 750,260 - RR42_RS24175 0.69 +0.4 750,260 - RR42_RS24175 0.69 -0.7 750,260 - RR42_RS24175 0.69 -0.3 750,340 - RR42_RS24175 0.75 -0.2 750,340 - RR42_RS24175 0.75 -0.8 750,586 - -0.2 750,586 - -0.2 750,659 + -1.0 750,659 + -0.7 750,660 - -0.3 750,660 - -1.1 750,702 + +1.4 750,703 - +0.3 750,706 + +0.2 750,706 + -0.2 750,707 - +1.5 750,742 + -1.3 750,742 + -0.2 750,742 + -0.1 750,742 + +0.6 750,931 + RR42_RS24180 0.31 -0.5 751,087 - RR42_RS24180 0.55 -0.7 751,167 + RR42_RS24180 0.68 -0.7 751,167 + RR42_RS24180 0.68 -1.2 751,168 - RR42_RS24180 0.68 +0.0 751,225 - RR42_RS24180 0.77 +1.8 751,254 + RR42_RS24180 0.81 -0.0 751,255 - RR42_RS24180 0.81 +1.1 751,379 + +0.2 751,379 + -1.2 751,379 + -0.4 751,379 + -0.3 751,379 + +0.9 751,380 - +0.5 751,457 + -1.2 751,458 - -0.0 751,472 + +0.7 751,499 + -1.0 751,521 - -0.4 751,557 + +0.9 751,558 - +1.0 751,601 + +0.6 751,617 + +0.0 751,618 - -0.4 751,630 + +0.7 751,630 + +0.8 751,669 + +0.1 751,669 + -0.2 751,692 + +0.2 751,692 + -0.5 751,734 - +0.2 751,770 + -0.5 751,770 + +0.7 751,770 + -0.2 751,770 + +0.4 751,771 - +0.5 751,771 - +0.0 751,953 + -0.9 751,953 + -2.2 751,954 - +0.6 751,988 - -0.5 751,988 - -0.4 752,019 + +0.1 752,019 + +0.1 752,020 - -0.0 752,020 - +0.4 752,150 - +0.8 752,179 - +1.6 752,216 + -0.2 752,217 - +0.4 752,228 + -0.5 752,282 + +0.2 752,282 + -0.0 752,311 + +1.0 752,311 + -0.2 752,312 - +0.8 752,359 - +0.0
Or see this region's nucleotide sequence