Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23680 and RR42_RS23685 are separated by 74 nucleotides RR42_RS23685 and RR42_RS23690 are separated by 132 nucleotides RR42_RS23690 and RR42_RS23695 are separated by 85 nucleotides
RR42_RS23680: RR42_RS23680 - LacI family transcriptional regulator, at 639,548 to 640,558
_RS23680
RR42_RS23685: RR42_RS23685 - transcriptional regulator, at 640,633 to 641,154
_RS23685
RR42_RS23690: RR42_RS23690 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase, at 641,287 to 642,054
_RS23690
RR42_RS23695: RR42_RS23695 - dihydroxynaphthoic acid synthetase, at 642,140 to 642,922
_RS23695
Position (kb) 640
641
642 Strain fitness (log2 ratio) -2
-1
0
1 at 639.911 kb on + strand, within RR42_RS23680 at 639.912 kb on - strand, within RR42_RS23680 at 640.049 kb on + strand, within RR42_RS23680 at 640.050 kb on - strand, within RR42_RS23680 at 640.136 kb on + strand, within RR42_RS23680 at 640.136 kb on + strand, within RR42_RS23680 at 640.136 kb on + strand, within RR42_RS23680 at 640.137 kb on - strand, within RR42_RS23680 at 640.137 kb on - strand, within RR42_RS23680 at 640.138 kb on + strand, within RR42_RS23680 at 640.391 kb on + strand, within RR42_RS23680 at 640.391 kb on + strand, within RR42_RS23680 at 640.392 kb on - strand, within RR42_RS23680 at 640.495 kb on + strand at 640.556 kb on + strand at 640.556 kb on + strand at 640.557 kb on - strand at 640.634 kb on + strand at 640.635 kb on - strand at 640.706 kb on + strand, within RR42_RS23685 at 640.707 kb on - strand, within RR42_RS23685 at 640.760 kb on + strand, within RR42_RS23685 at 640.761 kb on - strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.798 kb on + strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.799 kb on - strand, within RR42_RS23685 at 640.931 kb on + strand, within RR42_RS23685 at 640.931 kb on + strand, within RR42_RS23685 at 641.145 kb on + strand at 641.154 kb on + strand at 641.245 kb on - strand at 641.543 kb on + strand, within RR42_RS23690 at 641.544 kb on - strand, within RR42_RS23690 at 641.584 kb on - strand, within RR42_RS23690 at 641.749 kb on + strand, within RR42_RS23690 at 641.872 kb on + strand, within RR42_RS23690 at 641.873 kb on - strand, within RR42_RS23690 at 642.146 kb on + strand at 642.146 kb on + strand at 642.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 639,911 + RR42_RS23680 0.36 -0.0 639,912 - RR42_RS23680 0.36 +0.3 640,049 + RR42_RS23680 0.50 -0.6 640,050 - RR42_RS23680 0.50 +0.3 640,136 + RR42_RS23680 0.58 +0.4 640,136 + RR42_RS23680 0.58 -0.3 640,136 + RR42_RS23680 0.58 +0.6 640,137 - RR42_RS23680 0.58 +1.2 640,137 - RR42_RS23680 0.58 -0.0 640,138 + RR42_RS23680 0.58 -0.3 640,391 + RR42_RS23680 0.83 -0.6 640,391 + RR42_RS23680 0.83 -0.6 640,392 - RR42_RS23680 0.83 +0.2 640,495 + +0.2 640,556 + -0.6 640,556 + -0.2 640,557 - +0.5 640,634 + +0.2 640,635 - -0.1 640,706 + RR42_RS23685 0.14 -0.9 640,707 - RR42_RS23685 0.14 +0.3 640,760 + RR42_RS23685 0.24 -1.9 640,761 - RR42_RS23685 0.25 -0.7 640,798 + RR42_RS23685 0.32 -0.1 640,798 + RR42_RS23685 0.32 -1.1 640,798 + RR42_RS23685 0.32 -0.6 640,798 + RR42_RS23685 0.32 -0.4 640,798 + RR42_RS23685 0.32 -1.7 640,798 + RR42_RS23685 0.32 -2.1 640,799 - RR42_RS23685 0.32 -0.6 640,799 - RR42_RS23685 0.32 -0.9 640,799 - RR42_RS23685 0.32 +0.1 640,799 - RR42_RS23685 0.32 -0.5 640,799 - RR42_RS23685 0.32 -1.6 640,799 - RR42_RS23685 0.32 -1.4 640,799 - RR42_RS23685 0.32 -1.7 640,931 + RR42_RS23685 0.57 -1.0 640,931 + RR42_RS23685 0.57 -1.2 641,145 + -1.6 641,154 + -0.8 641,245 - +0.2 641,543 + RR42_RS23690 0.33 -0.6 641,544 - RR42_RS23690 0.33 -0.2 641,584 - RR42_RS23690 0.39 -0.2 641,749 + RR42_RS23690 0.60 +0.1 641,872 + RR42_RS23690 0.76 +1.0 641,873 - RR42_RS23690 0.76 +0.2 642,146 + +0.5 642,146 + -0.5 642,147 - -0.2
Or see this region's nucleotide sequence