Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS23455 and RR42_RS23460 overlap by 62 nucleotides RR42_RS23460 and RR42_RS23465 are separated by 230 nucleotides
RR42_RS23455: RR42_RS23455 - 6-carboxyhexanoate--CoA ligase, at 588,241 to 590,331
_RS23455
RR42_RS23460: RR42_RS23460 - alpha/beta hydrolase, at 590,270 to 590,644
_RS23460
RR42_RS23465: RR42_RS23465 - 2-methylcitrate dehydratase, at 590,875 to 592,209
_RS23465
Position (kb) 590
591 Strain fitness (log2 ratio) -1
0
1 at 589.378 kb on + strand, within RR42_RS23455 at 589.486 kb on + strand, within RR42_RS23455 at 590.204 kb on + strand at 590.574 kb on + strand, within RR42_RS23460 at 590.575 kb on - strand, within RR42_RS23460 at 590.661 kb on + strand at 590.662 kb on - strand at 590.662 kb on - strand at 590.783 kb on + strand at 590.783 kb on + strand at 590.843 kb on - strand at 591.166 kb on + strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.167 kb on - strand, within RR42_RS23465 at 591.184 kb on - strand, within RR42_RS23465 at 591.219 kb on - strand, within RR42_RS23465 at 591.219 kb on - strand, within RR42_RS23465 at 591.273 kb on + strand, within RR42_RS23465 at 591.314 kb on - strand, within RR42_RS23465 at 591.342 kb on - strand, within RR42_RS23465 at 591.373 kb on + strand, within RR42_RS23465 at 591.373 kb on + strand, within RR42_RS23465 at 591.585 kb on + strand, within RR42_RS23465 at 591.642 kb on + strand, within RR42_RS23465 at 591.642 kb on + strand, within RR42_RS23465 at 591.642 kb on + strand, within RR42_RS23465
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 589,378 + RR42_RS23455 0.54 -1.0 589,486 + RR42_RS23455 0.60 +0.3 590,204 + +0.9 590,574 + RR42_RS23460 0.81 -0.7 590,575 - RR42_RS23460 0.81 -1.1 590,661 + +0.5 590,662 - +0.2 590,662 - -0.1 590,783 + +0.4 590,783 + +0.2 590,843 - +1.5 591,166 + RR42_RS23465 0.22 -0.4 591,167 - RR42_RS23465 0.22 -1.2 591,167 - RR42_RS23465 0.22 +0.5 591,167 - RR42_RS23465 0.22 +0.8 591,184 - RR42_RS23465 0.23 -1.0 591,219 - RR42_RS23465 0.26 +0.5 591,219 - RR42_RS23465 0.26 -0.4 591,273 + RR42_RS23465 0.30 +1.2 591,314 - RR42_RS23465 0.33 -0.6 591,342 - RR42_RS23465 0.35 -0.6 591,373 + RR42_RS23465 0.37 +0.3 591,373 + RR42_RS23465 0.37 -0.3 591,585 + RR42_RS23465 0.53 +0.3 591,642 + RR42_RS23465 0.57 -0.0 591,642 + RR42_RS23465 0.57 +0.3 591,642 + RR42_RS23465 0.57 -0.5
Or see this region's nucleotide sequence