Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22840 and RR42_RS22845 are separated by 115 nucleotides RR42_RS22845 and RR42_RS22850 are separated by 118 nucleotides RR42_RS22850 and RR42_RS22855 are separated by 416 nucleotides RR42_RS22855 and RR42_RS22860 are separated by 81 nucleotides
RR42_RS22840: RR42_RS22840 - DeoR family transcriptional regulator, at 455,951 to 457,186
_RS22840
RR42_RS22845: RR42_RS22845 - cell envelope biogenesis protein OmpA, at 457,302 to 457,796
_RS22845
RR42_RS22850: RR42_RS22850 - amino acid dehydrogenase, at 457,915 to 458,958
_RS22850
RR42_RS22855: RR42_RS22855 - sulfate transporter, at 459,375 to 459,812
_RS22855
RR42_RS22860: RR42_RS22860 - l-threonine 3-dehydrogenase, at 459,894 to 460,949
_RS22860
Position (kb) 457
458
459 Strain fitness (log2 ratio) -2
-1
0
1
2 at 457.006 kb on + strand, within RR42_RS22840 at 457.006 kb on + strand, within RR42_RS22840 at 457.033 kb on - strand, within RR42_RS22840 at 457.276 kb on + strand at 457.276 kb on + strand at 457.277 kb on - strand at 457.277 kb on - strand at 457.589 kb on + strand, within RR42_RS22845 at 457.916 kb on + strand at 457.916 kb on + strand at 457.917 kb on - strand at 458.072 kb on + strand, within RR42_RS22850 at 458.072 kb on + strand, within RR42_RS22850 at 458.088 kb on + strand, within RR42_RS22850 at 458.088 kb on + strand, within RR42_RS22850 at 458.088 kb on + strand, within RR42_RS22850 at 458.126 kb on + strand, within RR42_RS22850 at 458.127 kb on - strand, within RR42_RS22850 at 458.181 kb on + strand, within RR42_RS22850 at 458.570 kb on + strand, within RR42_RS22850 at 458.571 kb on - strand, within RR42_RS22850 at 458.605 kb on + strand, within RR42_RS22850 at 458.606 kb on - strand, within RR42_RS22850 at 458.606 kb on - strand, within RR42_RS22850 at 458.636 kb on + strand, within RR42_RS22850 at 458.637 kb on - strand, within RR42_RS22850 at 458.765 kb on + strand, within RR42_RS22850 at 458.765 kb on + strand, within RR42_RS22850 at 458.766 kb on - strand, within RR42_RS22850 at 458.870 kb on + strand at 458.870 kb on + strand at 459.030 kb on + strand at 459.112 kb on - strand at 459.195 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.234 kb on + strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.235 kb on - strand at 459.282 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.284 kb on + strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.285 kb on - strand at 459.286 kb on + strand at 459.287 kb on - strand at 459.287 kb on - strand at 459.300 kb on + strand at 459.507 kb on + strand, within RR42_RS22855 at 459.507 kb on + strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.508 kb on - strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.513 kb on + strand, within RR42_RS22855 at 459.514 kb on - strand, within RR42_RS22855 at 459.646 kb on + strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.647 kb on - strand, within RR42_RS22855 at 459.661 kb on - strand, within RR42_RS22855 at 459.782 kb on + strand at 459.782 kb on + strand at 459.782 kb on + strand at 459.783 kb on - strand at 459.783 kb on - strand at 459.805 kb on - strand at 459.805 kb on - strand at 459.908 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 457,006 + RR42_RS22840 0.85 -0.1 457,006 + RR42_RS22840 0.85 -0.2 457,033 - RR42_RS22840 0.88 -0.2 457,276 + -0.2 457,276 + -0.2 457,277 - -0.3 457,277 - +0.3 457,589 + RR42_RS22845 0.58 +0.8 457,916 + +0.0 457,916 + -0.8 457,917 - -0.3 458,072 + RR42_RS22850 0.15 +0.2 458,072 + RR42_RS22850 0.15 +0.2 458,088 + RR42_RS22850 0.17 +0.5 458,088 + RR42_RS22850 0.17 +1.0 458,088 + RR42_RS22850 0.17 -0.3 458,126 + RR42_RS22850 0.20 -0.3 458,127 - RR42_RS22850 0.20 -0.6 458,181 + RR42_RS22850 0.25 +1.8 458,570 + RR42_RS22850 0.63 -0.0 458,571 - RR42_RS22850 0.63 -0.6 458,605 + RR42_RS22850 0.66 -0.4 458,606 - RR42_RS22850 0.66 -0.7 458,606 - RR42_RS22850 0.66 -0.5 458,636 + RR42_RS22850 0.69 +0.6 458,637 - RR42_RS22850 0.69 -0.1 458,765 + RR42_RS22850 0.81 +0.3 458,765 + RR42_RS22850 0.81 +1.4 458,766 - RR42_RS22850 0.82 -0.7 458,870 + -0.6 458,870 + +0.4 459,030 + -0.5 459,112 - -0.4 459,195 + -1.9 459,234 + -0.7 459,234 + -0.6 459,234 + +0.0 459,234 + -0.7 459,234 + -1.7 459,234 + -0.0 459,234 + -0.7 459,235 - -0.4 459,235 - +0.8 459,235 - +0.8 459,282 + -0.3 459,284 + -0.4 459,284 + -0.5 459,284 + -0.1 459,285 - +0.6 459,285 - -1.1 459,285 - +0.0 459,285 - +0.2 459,285 - +0.3 459,285 - -0.5 459,286 + +0.2 459,287 - -0.2 459,287 - -0.7 459,300 + +1.1 459,507 + RR42_RS22855 0.30 -0.4 459,507 + RR42_RS22855 0.30 -0.3 459,508 - RR42_RS22855 0.30 +1.1 459,508 - RR42_RS22855 0.30 +0.4 459,513 + RR42_RS22855 0.32 +0.4 459,513 + RR42_RS22855 0.32 +0.6 459,514 - RR42_RS22855 0.32 +0.0 459,646 + RR42_RS22855 0.62 -1.0 459,647 - RR42_RS22855 0.62 -0.1 459,647 - RR42_RS22855 0.62 -0.7 459,661 - RR42_RS22855 0.65 -0.4 459,782 + -0.5 459,782 + +0.1 459,782 + -0.0 459,783 - -2.0 459,783 - -0.5 459,805 - +0.4 459,805 - -0.4 459,908 + -1.5
Or see this region's nucleotide sequence