Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22730 and RR42_RS22735 are separated by 20 nucleotides RR42_RS22735 and RR42_RS22740 are separated by 121 nucleotides RR42_RS22740 and RR42_RS22745 are separated by 294 nucleotides
RR42_RS22730: RR42_RS22730 - pyridine nucleotide-disulfide oxidoreductase, at 433,813 to 435,018
_RS22730
RR42_RS22735: RR42_RS22735 - transcriptional regulator, at 435,039 to 435,578
_RS22735
RR42_RS22740: RR42_RS22740 - oxidoreductase, at 435,700 to 436,494
_RS22740
RR42_RS22745: RR42_RS22745 - phosphoribosylglycinamide formyltransferase, at 436,789 to 437,949
_RS22745
Position (kb) 435
436
437 Strain fitness (log2 ratio) -1
0
1 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.719 kb on + strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 434.720 kb on - strand, within RR42_RS22730 at 435.011 kb on + strand at 435.016 kb on + strand at 435.105 kb on + strand, within RR42_RS22735 at 435.105 kb on + strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.106 kb on - strand, within RR42_RS22735 at 435.452 kb on - strand, within RR42_RS22735 at 435.495 kb on - strand, within RR42_RS22735 at 435.605 kb on - strand at 435.615 kb on - strand at 435.617 kb on - strand at 435.712 kb on - strand at 435.780 kb on - strand, within RR42_RS22740 at 435.780 kb on - strand, within RR42_RS22740 at 436.034 kb on + strand, within RR42_RS22740 at 436.035 kb on - strand, within RR42_RS22740 at 436.035 kb on - strand, within RR42_RS22740 at 436.057 kb on - strand, within RR42_RS22740 at 436.101 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.124 kb on + strand, within RR42_RS22740 at 436.125 kb on - strand, within RR42_RS22740 at 436.234 kb on + strand, within RR42_RS22740 at 436.537 kb on + strand at 436.537 kb on + strand at 436.538 kb on - strand at 436.538 kb on - strand at 436.635 kb on + strand at 436.635 kb on + strand at 436.635 kb on + strand at 436.636 kb on - strand at 436.707 kb on + strand at 436.707 kb on + strand at 436.708 kb on - strand at 436.708 kb on - strand at 436.722 kb on + strand at 436.723 kb on - strand at 437.140 kb on + strand, within RR42_RS22745 at 437.287 kb on + strand, within RR42_RS22745 at 437.288 kb on - strand, within RR42_RS22745 at 437.353 kb on + strand, within RR42_RS22745
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 434,719 + RR42_RS22730 0.75 +0.7 434,719 + RR42_RS22730 0.75 +0.0 434,719 + RR42_RS22730 0.75 -0.9 434,720 - RR42_RS22730 0.75 +1.2 434,720 - RR42_RS22730 0.75 +0.5 435,011 + -0.8 435,016 + -0.6 435,105 + RR42_RS22735 0.12 +1.0 435,105 + RR42_RS22735 0.12 -0.3 435,106 - RR42_RS22735 0.12 -0.0 435,106 - RR42_RS22735 0.12 -0.8 435,452 - RR42_RS22735 0.76 -0.0 435,495 - RR42_RS22735 0.84 +0.8 435,605 - +0.1 435,615 - +1.0 435,617 - +0.5 435,712 - -0.2 435,780 - RR42_RS22740 0.10 +0.0 435,780 - RR42_RS22740 0.10 -0.2 436,034 + RR42_RS22740 0.42 +0.5 436,035 - RR42_RS22740 0.42 -0.6 436,035 - RR42_RS22740 0.42 +0.1 436,057 - RR42_RS22740 0.45 +0.1 436,101 + RR42_RS22740 0.50 +0.2 436,124 + RR42_RS22740 0.53 -0.1 436,124 + RR42_RS22740 0.53 -0.7 436,124 + RR42_RS22740 0.53 -1.5 436,125 - RR42_RS22740 0.53 -0.5 436,234 + RR42_RS22740 0.67 +0.2 436,537 + +1.5 436,537 + -0.1 436,538 - -0.2 436,538 - -0.3 436,635 + -0.5 436,635 + +0.9 436,635 + +0.3 436,636 - +0.1 436,707 + -0.1 436,707 + +1.0 436,708 - +0.2 436,708 - +1.0 436,722 + -1.4 436,723 - -0.7 437,140 + RR42_RS22745 0.30 +0.6 437,287 + RR42_RS22745 0.43 -0.7 437,288 - RR42_RS22745 0.43 -1.5 437,353 + RR42_RS22745 0.49 +0.3
Or see this region's nucleotide sequence