Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22385 and RR42_RS22390 are separated by 63 nucleotides RR42_RS22390 and RR42_RS22395 are separated by 312 nucleotides
RR42_RS22385: RR42_RS22385 - twin-arginine translocation pathway signal, at 358,521 to 359,498
_RS22385
RR42_RS22390: RR42_RS22390 - 3-oxoacyl-ACP reductase, at 359,562 to 360,344
_RS22390
RR42_RS22395: RR42_RS22395 - hypothetical protein, at 360,657 to 361,688
_RS22395
Position (kb) 359
360
361 Strain fitness (log2 ratio) -1
0
1
2 at 358.698 kb on + strand, within RR42_RS22385 at 358.729 kb on + strand, within RR42_RS22385 at 358.729 kb on + strand, within RR42_RS22385 at 358.730 kb on - strand, within RR42_RS22385 at 358.777 kb on + strand, within RR42_RS22385 at 358.777 kb on + strand, within RR42_RS22385 at 358.777 kb on + strand, within RR42_RS22385 at 358.815 kb on - strand, within RR42_RS22385 at 358.815 kb on - strand, within RR42_RS22385 at 358.893 kb on - strand, within RR42_RS22385 at 358.943 kb on + strand, within RR42_RS22385 at 358.943 kb on + strand, within RR42_RS22385 at 358.943 kb on + strand, within RR42_RS22385 at 358.943 kb on + strand, within RR42_RS22385 at 359.053 kb on + strand, within RR42_RS22385 at 359.054 kb on - strand, within RR42_RS22385 at 359.195 kb on + strand, within RR42_RS22385 at 359.195 kb on + strand, within RR42_RS22385 at 359.195 kb on + strand, within RR42_RS22385 at 359.227 kb on + strand, within RR42_RS22385 at 359.410 kb on + strand at 359.583 kb on + strand at 359.583 kb on + strand at 359.583 kb on + strand at 359.863 kb on + strand, within RR42_RS22390 at 359.864 kb on - strand, within RR42_RS22390 at 359.864 kb on - strand, within RR42_RS22390 at 359.864 kb on - strand, within RR42_RS22390 at 360.586 kb on + strand at 360.587 kb on - strand at 360.587 kb on - strand at 360.818 kb on + strand, within RR42_RS22395 at 361.153 kb on + strand, within RR42_RS22395
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 358,698 + RR42_RS22385 0.18 -0.6 358,729 + RR42_RS22385 0.21 -0.9 358,729 + RR42_RS22385 0.21 +0.1 358,730 - RR42_RS22385 0.21 +0.2 358,777 + RR42_RS22385 0.26 -0.7 358,777 + RR42_RS22385 0.26 -0.6 358,777 + RR42_RS22385 0.26 +2.5 358,815 - RR42_RS22385 0.30 +0.5 358,815 - RR42_RS22385 0.30 +0.5 358,893 - RR42_RS22385 0.38 -0.4 358,943 + RR42_RS22385 0.43 -0.1 358,943 + RR42_RS22385 0.43 -0.4 358,943 + RR42_RS22385 0.43 -0.1 358,943 + RR42_RS22385 0.43 -1.4 359,053 + RR42_RS22385 0.54 -0.1 359,054 - RR42_RS22385 0.54 -0.4 359,195 + RR42_RS22385 0.69 -1.0 359,195 + RR42_RS22385 0.69 -0.9 359,195 + RR42_RS22385 0.69 -0.1 359,227 + RR42_RS22385 0.72 -0.2 359,410 + -0.6 359,583 + +0.4 359,583 + -0.7 359,583 + +1.0 359,863 + RR42_RS22390 0.38 +0.3 359,864 - RR42_RS22390 0.39 -0.5 359,864 - RR42_RS22390 0.39 +0.5 359,864 - RR42_RS22390 0.39 -0.3 360,586 + +0.2 360,587 - -0.2 360,587 - -0.6 360,818 + RR42_RS22395 0.16 -0.5 361,153 + RR42_RS22395 0.48 -0.5
Or see this region's nucleotide sequence