Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22095 and RR42_RS22100 are separated by 496 nucleotides RR42_RS22100 and RR42_RS22105 are separated by 46 nucleotides
RR42_RS22095: RR42_RS22095 - hypothetical protein, at 293,215 to 294,285
_RS22095
RR42_RS22100: RR42_RS22100 - type VI secretion protein VasK, at 294,782 to 298,351
_RS22100
RR42_RS22105: RR42_RS22105 - type VI secretion protein, at 298,398 to 299,972
_RS22105
Position (kb) 294
295
296
297
298
299 Strain fitness (log2 ratio) -1
0
1 at 294.072 kb on - strand, within RR42_RS22095 at 294.398 kb on + strand at 294.398 kb on + strand at 294.412 kb on + strand at 294.423 kb on - strand at 294.423 kb on - strand at 294.502 kb on + strand at 294.667 kb on + strand at 294.667 kb on + strand at 294.667 kb on + strand at 294.668 kb on - strand at 294.668 kb on - strand at 294.668 kb on - strand at 294.676 kb on + strand at 294.792 kb on + strand at 294.808 kb on - strand at 294.816 kb on + strand at 294.817 kb on - strand at 296.826 kb on - strand, within RR42_RS22100 at 296.838 kb on - strand, within RR42_RS22100 at 296.838 kb on - strand, within RR42_RS22100 at 298.656 kb on + strand, within RR42_RS22105 at 298.656 kb on + strand, within RR42_RS22105 at 298.657 kb on - strand, within RR42_RS22105 at 298.657 kb on - strand, within RR42_RS22105 at 298.659 kb on + strand, within RR42_RS22105 at 298.659 kb on + strand, within RR42_RS22105 at 298.660 kb on - strand, within RR42_RS22105 at 298.661 kb on - strand, within RR42_RS22105 at 298.665 kb on + strand, within RR42_RS22105 at 298.866 kb on - strand, within RR42_RS22105 at 299.100 kb on + strand, within RR42_RS22105 at 299.100 kb on + strand, within RR42_RS22105 at 299.125 kb on - strand, within RR42_RS22105 at 299.155 kb on + strand, within RR42_RS22105 at 299.166 kb on - strand, within RR42_RS22105 at 299.343 kb on + strand, within RR42_RS22105 at 299.344 kb on - strand, within RR42_RS22105 at 299.346 kb on + strand, within RR42_RS22105 at 299.347 kb on - strand, within RR42_RS22105
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 294,072 - RR42_RS22095 0.80 +0.0 294,398 + +1.2 294,398 + +0.9 294,412 + +0.3 294,423 - -0.9 294,423 - +0.6 294,502 + -1.0 294,667 + -0.8 294,667 + +0.5 294,667 + +0.9 294,668 - -0.0 294,668 - -0.0 294,668 - +0.2 294,676 + -0.4 294,792 + -0.9 294,808 - +1.6 294,816 + -0.1 294,817 - -0.3 296,826 - RR42_RS22100 0.57 +1.0 296,838 - RR42_RS22100 0.58 +0.4 296,838 - RR42_RS22100 0.58 +0.1 298,656 + RR42_RS22105 0.16 -0.5 298,656 + RR42_RS22105 0.16 +0.4 298,657 - RR42_RS22105 0.16 -0.4 298,657 - RR42_RS22105 0.16 +0.4 298,659 + RR42_RS22105 0.17 +0.7 298,659 + RR42_RS22105 0.17 -0.1 298,660 - RR42_RS22105 0.17 +0.3 298,661 - RR42_RS22105 0.17 +0.7 298,665 + RR42_RS22105 0.17 +0.5 298,866 - RR42_RS22105 0.30 +0.0 299,100 + RR42_RS22105 0.45 -0.9 299,100 + RR42_RS22105 0.45 +0.5 299,125 - RR42_RS22105 0.46 +0.0 299,155 + RR42_RS22105 0.48 +0.4 299,166 - RR42_RS22105 0.49 +0.6 299,343 + RR42_RS22105 0.60 -0.1 299,344 - RR42_RS22105 0.60 +0.3 299,346 + RR42_RS22105 0.60 -0.2 299,347 - RR42_RS22105 0.60 +0.0
Or see this region's nucleotide sequence