Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21860 and RR42_RS21865 are separated by 155 nucleotides RR42_RS21865 and RR42_RS21870 are separated by 165 nucleotides
RR42_RS21860: RR42_RS21860 - hypothetical protein, at 230,852 to 231,661
_RS21860
RR42_RS21865: RR42_RS21865 - 3-ketoacyl-ACP reductase, at 231,817 to 232,560
_RS21865
RR42_RS21870: RR42_RS21870 - transcriptional regulator, at 232,726 to 233,607
_RS21870
Position (kb) 231
232
233 Strain fitness (log2 ratio) -2
-1
0
1
2 at 230.873 kb on + strand at 230.873 kb on + strand at 230.873 kb on + strand at 230.874 kb on - strand at 230.874 kb on - strand at 230.874 kb on - strand at 230.874 kb on - strand at 230.874 kb on - strand at 230.875 kb on + strand at 231.114 kb on + strand, within RR42_RS21860 at 231.114 kb on + strand, within RR42_RS21860 at 231.114 kb on + strand, within RR42_RS21860 at 231.114 kb on + strand, within RR42_RS21860 at 231.114 kb on + strand, within RR42_RS21860 at 231.115 kb on - strand, within RR42_RS21860 at 231.115 kb on - strand, within RR42_RS21860 at 231.115 kb on - strand, within RR42_RS21860 at 231.129 kb on + strand, within RR42_RS21860 at 231.130 kb on - strand, within RR42_RS21860 at 231.191 kb on + strand, within RR42_RS21860 at 231.191 kb on + strand, within RR42_RS21860 at 231.203 kb on - strand, within RR42_RS21860 at 231.418 kb on - strand, within RR42_RS21860 at 231.587 kb on + strand at 231.704 kb on + strand at 231.704 kb on + strand at 231.704 kb on + strand at 231.718 kb on + strand at 232.097 kb on + strand, within RR42_RS21865 at 232.097 kb on + strand, within RR42_RS21865 at 232.097 kb on + strand, within RR42_RS21865 at 232.098 kb on - strand, within RR42_RS21865 at 232.107 kb on - strand, within RR42_RS21865 at 232.235 kb on + strand, within RR42_RS21865 at 232.235 kb on + strand, within RR42_RS21865 at 232.235 kb on + strand, within RR42_RS21865 at 232.236 kb on - strand, within RR42_RS21865 at 232.236 kb on - strand, within RR42_RS21865 at 232.236 kb on - strand, within RR42_RS21865 at 232.448 kb on + strand at 232.448 kb on + strand, within RR42_RS21865 at 232.449 kb on - strand, within RR42_RS21865 at 232.449 kb on - strand, within RR42_RS21865 at 232.449 kb on - strand, within RR42_RS21865 at 232.449 kb on - strand, within RR42_RS21865 at 232.579 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.590 kb on + strand at 232.591 kb on - strand at 232.591 kb on - strand at 232.591 kb on - strand at 232.636 kb on - strand at 232.921 kb on + strand, within RR42_RS21870 at 233.230 kb on + strand, within RR42_RS21870 at 233.230 kb on + strand, within RR42_RS21870 at 233.293 kb on + strand, within RR42_RS21870 at 233.293 kb on + strand, within RR42_RS21870 at 233.293 kb on + strand, within RR42_RS21870 at 233.293 kb on + strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.294 kb on - strand, within RR42_RS21870 at 233.401 kb on + strand, within RR42_RS21870 at 233.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 230,873 + +0.7 230,873 + +0.0 230,873 + +0.3 230,874 - +0.4 230,874 - -0.2 230,874 - -0.7 230,874 - -0.7 230,874 - +0.7 230,875 + -0.6 231,114 + RR42_RS21860 0.32 -1.2 231,114 + RR42_RS21860 0.32 +0.6 231,114 + RR42_RS21860 0.32 -0.3 231,114 + RR42_RS21860 0.32 -0.0 231,114 + RR42_RS21860 0.32 -0.5 231,115 - RR42_RS21860 0.32 +0.5 231,115 - RR42_RS21860 0.32 -0.3 231,115 - RR42_RS21860 0.32 -0.9 231,129 + RR42_RS21860 0.34 +0.6 231,130 - RR42_RS21860 0.34 -1.8 231,191 + RR42_RS21860 0.42 +0.4 231,191 + RR42_RS21860 0.42 -0.8 231,203 - RR42_RS21860 0.43 +0.1 231,418 - RR42_RS21860 0.70 -1.9 231,587 + -0.4 231,704 + -0.7 231,704 + -0.6 231,704 + -0.2 231,718 + +0.4 232,097 + RR42_RS21865 0.38 +0.9 232,097 + RR42_RS21865 0.38 -1.2 232,097 + RR42_RS21865 0.38 +0.4 232,098 - RR42_RS21865 0.38 +0.2 232,107 - RR42_RS21865 0.39 -1.1 232,235 + RR42_RS21865 0.56 -1.5 232,235 + RR42_RS21865 0.56 +1.3 232,235 + RR42_RS21865 0.56 +0.3 232,236 - RR42_RS21865 0.56 +0.7 232,236 - RR42_RS21865 0.56 +0.7 232,236 - RR42_RS21865 0.56 -0.1 232,448 + +0.9 232,448 + RR42_RS21865 0.85 -0.9 232,449 - RR42_RS21865 0.85 +0.9 232,449 - RR42_RS21865 0.85 +0.8 232,449 - RR42_RS21865 0.85 -0.1 232,449 - RR42_RS21865 0.85 -0.5 232,579 + -0.1 232,590 + +0.5 232,590 + -0.3 232,590 + +0.6 232,590 + +0.3 232,590 + -0.2 232,590 + +0.9 232,590 + -0.1 232,590 + -0.2 232,590 + -0.7 232,591 - -0.7 232,591 - +0.4 232,591 - +0.2 232,636 - -0.2 232,921 + RR42_RS21870 0.22 +0.7 233,230 + RR42_RS21870 0.57 -0.0 233,230 + RR42_RS21870 0.57 +0.7 233,293 + RR42_RS21870 0.64 +2.5 233,293 + RR42_RS21870 0.64 -0.7 233,293 + RR42_RS21870 0.64 +0.7 233,293 + RR42_RS21870 0.64 -1.0 233,294 - RR42_RS21870 0.64 +0.2 233,294 - RR42_RS21870 0.64 -0.1 233,294 - RR42_RS21870 0.64 -0.5 233,294 - RR42_RS21870 0.64 +0.4 233,294 - RR42_RS21870 0.64 -0.0 233,294 - RR42_RS21870 0.64 +0.8 233,401 + RR42_RS21870 0.77 -0.1 233,555 + +0.6
Or see this region's nucleotide sequence