Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21775 and RR42_RS21780 are separated by 1 nucleotides RR42_RS21780 and RR42_RS21785 are separated by 45 nucleotides RR42_RS21785 and RR42_RS21790 are separated by 58 nucleotides RR42_RS21790 and RR42_RS21795 are separated by 62 nucleotides
RR42_RS21775: RR42_RS21775 - branched-chain alpha-keto acid dehydrogenase subunit E2, at 214,176 to 215,408
_RS21775
RR42_RS21780: RR42_RS21780 - bleomycin resistance protein, at 215,410 to 215,895
_RS21780
RR42_RS21785: RR42_RS21785 - MFS transporter, at 215,941 to 218,130
_RS21785
RR42_RS21790: RR42_RS21790 - short-chain dehydrogenase, at 218,189 to 218,947
_RS21790
RR42_RS21795: RR42_RS21795 - mannosyltransferase, at 219,010 to 219,744
_RS21795
Position (kb)
215
216
217
218
219 Strain fitness (log2 ratio)
-2
-1
0
1 at 215.325 kb on - strand at 215.395 kb on - strand at 215.510 kb on + strand, within RR42_RS21780 at 215.511 kb on - strand, within RR42_RS21780 at 215.511 kb on - strand, within RR42_RS21780 at 215.607 kb on + strand, within RR42_RS21780 at 215.608 kb on - strand, within RR42_RS21780 at 215.619 kb on - strand, within RR42_RS21780 at 215.645 kb on + strand, within RR42_RS21780 at 215.645 kb on + strand, within RR42_RS21780 at 215.646 kb on - strand, within RR42_RS21780 at 215.823 kb on - strand, within RR42_RS21780 at 215.899 kb on + strand at 216.093 kb on - strand at 216.163 kb on + strand, within RR42_RS21785 at 216.164 kb on - strand, within RR42_RS21785 at 216.302 kb on + strand, within RR42_RS21785 at 216.303 kb on - strand, within RR42_RS21785 at 216.303 kb on - strand, within RR42_RS21785 at 216.303 kb on - strand, within RR42_RS21785 at 216.365 kb on + strand, within RR42_RS21785 at 216.404 kb on + strand, within RR42_RS21785 at 216.560 kb on + strand, within RR42_RS21785 at 216.687 kb on - strand, within RR42_RS21785 at 217.290 kb on - strand, within RR42_RS21785 at 217.361 kb on + strand, within RR42_RS21785 at 217.361 kb on + strand, within RR42_RS21785 at 217.405 kb on - strand, within RR42_RS21785 at 217.577 kb on + strand, within RR42_RS21785 at 218.119 kb on - strand at 218.392 kb on - strand, within RR42_RS21790 at 218.425 kb on - strand, within RR42_RS21790 at 218.487 kb on + strand, within RR42_RS21790 at 218.996 kb on - strand at 219.013 kb on + strand at 219.013 kb on + strand at 219.014 kb on - strand at 219.042 kb on + strand at 219.116 kb on - strand, within RR42_RS21795
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 215,325 - +0.3 215,395 - +0.1 215,510 + RR42_RS21780 0.21 -0.1 215,511 - RR42_RS21780 0.21 -0.4 215,511 - RR42_RS21780 0.21 +0.4 215,607 + RR42_RS21780 0.41 +0.4 215,608 - RR42_RS21780 0.41 +0.1 215,619 - RR42_RS21780 0.43 +0.5 215,645 + RR42_RS21780 0.48 -0.2 215,645 + RR42_RS21780 0.48 +0.4 215,646 - RR42_RS21780 0.49 +0.1 215,823 - RR42_RS21780 0.85 -0.2 215,899 + -0.3 216,093 - -0.9 216,163 + RR42_RS21785 0.10 -0.3 216,164 - RR42_RS21785 0.10 -0.3 216,302 + RR42_RS21785 0.16 +0.4 216,303 - RR42_RS21785 0.17 +0.4 216,303 - RR42_RS21785 0.17 +0.1 216,303 - RR42_RS21785 0.17 +1.6 216,365 + RR42_RS21785 0.19 -0.3 216,404 + RR42_RS21785 0.21 +0.4 216,560 + RR42_RS21785 0.28 -2.2 216,687 - RR42_RS21785 0.34 -0.6 217,290 - RR42_RS21785 0.62 +0.8 217,361 + RR42_RS21785 0.65 -0.2 217,361 + RR42_RS21785 0.65 -0.3 217,405 - RR42_RS21785 0.67 +0.0 217,577 + RR42_RS21785 0.75 -0.9 218,119 - -0.0 218,392 - RR42_RS21790 0.27 +0.3 218,425 - RR42_RS21790 0.31 +1.3 218,487 + RR42_RS21790 0.39 +0.1 218,996 - -0.4 219,013 + +0.1 219,013 + -2.4 219,014 - +0.1 219,042 + -0.3 219,116 - RR42_RS21795 0.14 -0.3
Or see this region's nucleotide sequence