Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21290 and RR42_RS21295 overlap by 4 nucleotides RR42_RS21295 and RR42_RS21300 are separated by 138 nucleotides RR42_RS21300 and RR42_RS21305 are separated by 179 nucleotides
RR42_RS21290: RR42_RS21290 - L-lactate dehydrogenase, LutC subunit <small>(from data)</small>, at 103,577 to 104,281
_RS21290
RR42_RS21295: RR42_RS21295 - L-lactate dehydrogenase, LutA subunit <small>(from data)</small>, at 104,278 to 105,066
_RS21295
RR42_RS21300: RR42_RS21300 - GntR family transcriptional regulator, at 105,205 to 105,930
_RS21300
RR42_RS21305: RR42_RS21305 - membrane protein, at 106,110 to 107,147
_RS21305
Position (kb) 105
106 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 104.240 kb on + strand at 104.711 kb on + strand, within RR42_RS21295 at 104.895 kb on + strand, within RR42_RS21295 at 104.896 kb on - strand, within RR42_RS21295 at 105.026 kb on + strand at 105.130 kb on + strand at 105.331 kb on + strand, within RR42_RS21300 at 105.331 kb on + strand, within RR42_RS21300 at 105.332 kb on - strand, within RR42_RS21300 at 105.711 kb on + strand, within RR42_RS21300 at 105.711 kb on + strand, within RR42_RS21300 at 105.712 kb on - strand, within RR42_RS21300 at 105.794 kb on + strand, within RR42_RS21300 at 105.795 kb on - strand, within RR42_RS21300 at 105.795 kb on - strand, within RR42_RS21300 at 106.156 kb on + strand at 106.156 kb on + strand at 106.157 kb on - strand at 106.157 kb on - strand at 106.190 kb on - strand at 106.190 kb on - strand at 106.464 kb on + strand, within RR42_RS21305 at 106.477 kb on + strand, within RR42_RS21305 at 106.477 kb on + strand, within RR42_RS21305 at 106.478 kb on - strand, within RR42_RS21305 at 106.619 kb on - strand, within RR42_RS21305 at 106.847 kb on + strand, within RR42_RS21305 at 106.848 kb on - strand, within RR42_RS21305
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 104,240 + +0.9 104,711 + RR42_RS21295 0.55 -2.4 104,895 + RR42_RS21295 0.78 -3.7 104,896 - RR42_RS21295 0.78 -1.4 105,026 + -2.0 105,130 + -2.4 105,331 + RR42_RS21300 0.17 -0.1 105,331 + RR42_RS21300 0.17 -1.2 105,332 - RR42_RS21300 0.17 -0.2 105,711 + RR42_RS21300 0.70 -2.7 105,711 + RR42_RS21300 0.70 -1.3 105,712 - RR42_RS21300 0.70 -3.6 105,794 + RR42_RS21300 0.81 -3.1 105,795 - RR42_RS21300 0.81 -2.6 105,795 - RR42_RS21300 0.81 -2.2 106,156 + -0.2 106,156 + +0.1 106,157 - -0.0 106,157 - -1.9 106,190 - -0.1 106,190 - -0.9 106,464 + RR42_RS21305 0.34 +3.1 106,477 + RR42_RS21305 0.35 -0.7 106,477 + RR42_RS21305 0.35 +0.1 106,478 - RR42_RS21305 0.35 -0.2 106,619 - RR42_RS21305 0.49 +1.3 106,847 + RR42_RS21305 0.71 -2.8 106,848 - RR42_RS21305 0.71 +1.0
Or see this region's nucleotide sequence