Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21200 and RR42_RS21205 are separated by 357 nucleotides RR42_RS21205 and RR42_RS21210 are separated by 856 nucleotides
RR42_RS21200: RR42_RS21200 - membrane protein, at 79,901 to 80,545
_RS21200
RR42_RS21205: RR42_RS21205 - 2-isopropylmalate synthase, at 80,903 to 82,600
_RS21205
RR42_RS21210: RR42_RS21210 - hypothetical protein, at 83,457 to 84,767
_RS21210
Position (kb)
80
81
82
83 Strain fitness (log2 ratio)
-1
0
1
2
3 at 80.043 kb on + strand, within RR42_RS21200 at 80.044 kb on - strand, within RR42_RS21200 at 80.084 kb on - strand, within RR42_RS21200 at 80.154 kb on + strand, within RR42_RS21200 at 80.154 kb on + strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.155 kb on - strand, within RR42_RS21200 at 80.188 kb on - strand, within RR42_RS21200 at 80.247 kb on + strand, within RR42_RS21200 at 80.311 kb on - strand, within RR42_RS21200 at 80.410 kb on - strand, within RR42_RS21200 at 80.529 kb on + strand at 80.530 kb on - strand at 80.530 kb on - strand at 80.727 kb on - strand at 80.742 kb on + strand at 81.057 kb on + strand at 81.057 kb on + strand at 81.057 kb on + strand at 81.058 kb on - strand at 81.106 kb on - strand, within RR42_RS21205 at 81.272 kb on - strand, within RR42_RS21205 at 81.273 kb on + strand, within RR42_RS21205 at 81.273 kb on + strand, within RR42_RS21205 at 81.274 kb on - strand, within RR42_RS21205 at 81.274 kb on - strand, within RR42_RS21205 at 81.321 kb on + strand, within RR42_RS21205 at 81.321 kb on + strand, within RR42_RS21205 at 81.321 kb on + strand, within RR42_RS21205 at 81.322 kb on - strand, within RR42_RS21205 at 81.409 kb on + strand, within RR42_RS21205 at 81.410 kb on - strand, within RR42_RS21205 at 81.410 kb on - strand, within RR42_RS21205 at 81.411 kb on + strand, within RR42_RS21205 at 81.411 kb on + strand, within RR42_RS21205 at 81.411 kb on + strand, within RR42_RS21205 at 81.412 kb on - strand, within RR42_RS21205 at 81.412 kb on - strand, within RR42_RS21205 at 81.438 kb on + strand, within RR42_RS21205 at 81.438 kb on + strand, within RR42_RS21205 at 81.470 kb on + strand, within RR42_RS21205 at 81.489 kb on + strand, within RR42_RS21205 at 81.525 kb on + strand, within RR42_RS21205 at 81.526 kb on - strand, within RR42_RS21205 at 81.627 kb on + strand, within RR42_RS21205 at 81.627 kb on + strand, within RR42_RS21205 at 81.627 kb on + strand, within RR42_RS21205 at 81.627 kb on + strand, within RR42_RS21205 at 81.628 kb on - strand, within RR42_RS21205 at 81.628 kb on - strand, within RR42_RS21205 at 81.729 kb on + strand, within RR42_RS21205 at 81.881 kb on + strand, within RR42_RS21205 at 81.882 kb on - strand, within RR42_RS21205 at 81.882 kb on - strand, within RR42_RS21205 at 81.882 kb on - strand, within RR42_RS21205 at 81.882 kb on - strand, within RR42_RS21205 at 81.940 kb on + strand, within RR42_RS21205 at 81.940 kb on + strand, within RR42_RS21205 at 82.081 kb on - strand, within RR42_RS21205 at 82.086 kb on + strand, within RR42_RS21205 at 82.086 kb on + strand, within RR42_RS21205 at 82.087 kb on - strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.218 kb on + strand, within RR42_RS21205 at 82.219 kb on - strand, within RR42_RS21205 at 82.219 kb on - strand, within RR42_RS21205 at 82.219 kb on - strand, within RR42_RS21205 at 82.219 kb on - strand, within RR42_RS21205 at 82.271 kb on + strand, within RR42_RS21205 at 82.553 kb on - strand at 82.553 kb on - strand at 82.556 kb on + strand at 82.556 kb on + strand at 82.556 kb on + strand at 82.557 kb on - strand at 82.557 kb on - strand at 82.557 kb on - strand at 82.557 kb on - strand at 82.764 kb on + strand at 82.764 kb on + strand at 82.765 kb on - strand at 82.765 kb on - strand at 82.772 kb on - strand at 82.791 kb on + strand at 82.792 kb on - strand at 82.797 kb on + strand at 82.797 kb on + strand at 82.798 kb on - strand at 82.798 kb on - strand at 83.009 kb on - strand at 83.017 kb on + strand at 83.017 kb on + strand at 83.017 kb on + strand at 83.018 kb on - strand at 83.018 kb on - strand at 83.040 kb on + strand at 83.041 kb on - strand at 83.041 kb on - strand at 83.041 kb on - strand at 83.092 kb on + strand at 83.093 kb on - strand at 83.217 kb on + strand at 83.217 kb on + strand at 83.218 kb on - strand at 83.218 kb on - strand at 83.218 kb on - strand at 83.218 kb on - strand at 83.218 kb on - strand at 83.221 kb on + strand at 83.221 kb on + strand at 83.221 kb on + strand at 83.221 kb on + strand at 83.222 kb on - strand at 83.222 kb on - strand at 83.222 kb on - strand at 83.222 kb on - strand at 83.252 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 80,043 + RR42_RS21200 0.22 +0.3 80,044 - RR42_RS21200 0.22 +0.1 80,084 - RR42_RS21200 0.28 +0.7 80,154 + RR42_RS21200 0.39 -0.5 80,154 + RR42_RS21200 0.39 -0.2 80,155 - RR42_RS21200 0.39 +0.1 80,155 - RR42_RS21200 0.39 +0.2 80,155 - RR42_RS21200 0.39 -0.6 80,188 - RR42_RS21200 0.44 -1.7 80,247 + RR42_RS21200 0.54 -0.3 80,311 - RR42_RS21200 0.64 +0.6 80,410 - RR42_RS21200 0.79 -0.3 80,529 + +0.3 80,530 - -0.8 80,530 - +0.5 80,727 - +0.2 80,742 + -0.2 81,057 + -0.1 81,057 + +0.3 81,057 + +1.0 81,058 - +3.1 81,106 - RR42_RS21205 0.12 +1.0 81,272 - RR42_RS21205 0.22 -1.6 81,273 + RR42_RS21205 0.22 -0.0 81,273 + RR42_RS21205 0.22 +2.5 81,274 - RR42_RS21205 0.22 -0.2 81,274 - RR42_RS21205 0.22 -0.1 81,321 + RR42_RS21205 0.25 +1.7 81,321 + RR42_RS21205 0.25 -0.2 81,321 + RR42_RS21205 0.25 -0.1 81,322 - RR42_RS21205 0.25 +0.8 81,409 + RR42_RS21205 0.30 +1.5 81,410 - RR42_RS21205 0.30 +1.5 81,410 - RR42_RS21205 0.30 -0.7 81,411 + RR42_RS21205 0.30 +0.7 81,411 + RR42_RS21205 0.30 -0.1 81,411 + RR42_RS21205 0.30 +0.2 81,412 - RR42_RS21205 0.30 -0.3 81,412 - RR42_RS21205 0.30 +0.6 81,438 + RR42_RS21205 0.32 -0.6 81,438 + RR42_RS21205 0.32 -0.1 81,470 + RR42_RS21205 0.33 -1.0 81,489 + RR42_RS21205 0.35 +0.7 81,525 + RR42_RS21205 0.37 +0.2 81,526 - RR42_RS21205 0.37 -1.1 81,627 + RR42_RS21205 0.43 -0.4 81,627 + RR42_RS21205 0.43 -0.1 81,627 + RR42_RS21205 0.43 -0.7 81,627 + RR42_RS21205 0.43 -0.4 81,628 - RR42_RS21205 0.43 -0.3 81,628 - RR42_RS21205 0.43 -0.7 81,729 + RR42_RS21205 0.49 +1.1 81,881 + RR42_RS21205 0.58 +0.3 81,882 - RR42_RS21205 0.58 +0.1 81,882 - RR42_RS21205 0.58 -0.5 81,882 - RR42_RS21205 0.58 +0.5 81,882 - RR42_RS21205 0.58 -0.1 81,940 + RR42_RS21205 0.61 +1.5 81,940 + RR42_RS21205 0.61 -0.6 82,081 - RR42_RS21205 0.69 -0.3 82,086 + RR42_RS21205 0.70 -0.2 82,086 + RR42_RS21205 0.70 -0.0 82,087 - RR42_RS21205 0.70 +0.2 82,218 + RR42_RS21205 0.77 +0.6 82,218 + RR42_RS21205 0.77 -0.1 82,218 + RR42_RS21205 0.77 -0.3 82,218 + RR42_RS21205 0.77 -0.6 82,218 + RR42_RS21205 0.77 -0.4 82,218 + RR42_RS21205 0.77 -0.4 82,219 - RR42_RS21205 0.78 -0.9 82,219 - RR42_RS21205 0.78 +0.3 82,219 - RR42_RS21205 0.78 -0.2 82,219 - RR42_RS21205 0.78 +0.6 82,271 + RR42_RS21205 0.81 +1.1 82,553 - -1.0 82,553 - -0.4 82,556 + -0.1 82,556 + +0.1 82,556 + +0.7 82,557 - +0.3 82,557 - -1.3 82,557 - -0.8 82,557 - -0.1 82,764 + -0.1 82,764 + -0.7 82,765 - +0.5 82,765 - +0.1 82,772 - -0.6 82,791 + +0.7 82,792 - -1.4 82,797 + -1.0 82,797 + +2.9 82,798 - +0.4 82,798 - -0.4 83,009 - +0.1 83,017 + +0.4 83,017 + +0.3 83,017 + -0.4 83,018 - +0.2 83,018 - +0.8 83,040 + -0.1 83,041 - -0.3 83,041 - +0.6 83,041 - +0.0 83,092 + -1.3 83,093 - -0.4 83,217 + -0.3 83,217 + -0.4 83,218 - -0.4 83,218 - +0.4 83,218 - +0.5 83,218 - -0.4 83,218 - +0.7 83,221 + +1.9 83,221 + -0.3 83,221 + +0.1 83,221 + -0.3 83,222 - +0.5 83,222 - -0.0 83,222 - -0.2 83,222 - -0.0 83,252 - -0.2
Or see this region's nucleotide sequence