Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS21120 and RR42_RS21125 are separated by 249 nucleotides RR42_RS21125 and RR42_RS21130 are separated by 248 nucleotides
RR42_RS21120: RR42_RS21120 - acetate kinase, at 63,333 to 64,745
_RS21120
RR42_RS21125: RR42_RS21125 - membrane protein, at 64,995 to 65,507
_RS21125
RR42_RS21130: RR42_RS21130 - ABC transporter permease, at 65,756 to 66,892
_RS21130
Position (kb) 64
65
66 Strain fitness (log2 ratio) -1
0
1 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.025 kb on - strand, within RR42_RS21120 at 64.042 kb on - strand, within RR42_RS21120 at 64.155 kb on + strand, within RR42_RS21120 at 64.167 kb on + strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.168 kb on - strand, within RR42_RS21120 at 64.209 kb on + strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.210 kb on - strand, within RR42_RS21120 at 64.347 kb on - strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.395 kb on + strand, within RR42_RS21120 at 64.635 kb on + strand at 64.660 kb on - strand at 64.827 kb on - strand at 64.827 kb on - strand at 64.842 kb on + strand at 64.843 kb on - strand at 65.006 kb on + strand at 65.006 kb on + strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.007 kb on - strand at 65.117 kb on + strand, within RR42_RS21125 at 65.118 kb on - strand, within RR42_RS21125 at 65.614 kb on - strand at 65.681 kb on + strand at 65.682 kb on - strand at 65.682 kb on - strand at 65.931 kb on - strand, within RR42_RS21130 at 65.931 kb on - strand, within RR42_RS21130 at 65.962 kb on - strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.990 kb on + strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 65.991 kb on - strand, within RR42_RS21130 at 66.076 kb on - strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.198 kb on + strand, within RR42_RS21130 at 66.199 kb on - strand, within RR42_RS21130 at 66.209 kb on + strand, within RR42_RS21130 at 66.279 kb on - strand, within RR42_RS21130
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 64,025 - RR42_RS21120 0.49 -0.4 64,025 - RR42_RS21120 0.49 +0.5 64,025 - RR42_RS21120 0.49 -0.8 64,025 - RR42_RS21120 0.49 -0.1 64,042 - RR42_RS21120 0.50 +0.1 64,155 + RR42_RS21120 0.58 -1.4 64,167 + RR42_RS21120 0.59 -0.3 64,168 - RR42_RS21120 0.59 -0.7 64,168 - RR42_RS21120 0.59 +0.3 64,209 + RR42_RS21120 0.62 -0.4 64,210 - RR42_RS21120 0.62 +0.4 64,210 - RR42_RS21120 0.62 +1.3 64,347 - RR42_RS21120 0.72 +0.5 64,395 + RR42_RS21120 0.75 -0.2 64,395 + RR42_RS21120 0.75 +0.5 64,395 + RR42_RS21120 0.75 +0.6 64,635 + -0.2 64,660 - +0.2 64,827 - +0.5 64,827 - +0.6 64,842 + -1.1 64,843 - +0.3 65,006 + +1.2 65,006 + +1.1 65,007 - -0.0 65,007 - -0.3 65,007 - -0.7 65,117 + RR42_RS21125 0.24 -0.1 65,118 - RR42_RS21125 0.24 +0.9 65,614 - -0.4 65,681 + +0.9 65,682 - +0.1 65,682 - -0.1 65,931 - RR42_RS21130 0.15 +0.6 65,931 - RR42_RS21130 0.15 -0.7 65,962 - RR42_RS21130 0.18 -0.1 65,990 + RR42_RS21130 0.21 +1.1 65,990 + RR42_RS21130 0.21 -0.1 65,991 - RR42_RS21130 0.21 +0.5 65,991 - RR42_RS21130 0.21 +0.2 66,076 - RR42_RS21130 0.28 +0.9 66,198 + RR42_RS21130 0.39 -0.7 66,198 + RR42_RS21130 0.39 +0.3 66,198 + RR42_RS21130 0.39 -0.4 66,199 - RR42_RS21130 0.39 +0.1 66,209 + RR42_RS21130 0.40 -1.0 66,279 - RR42_RS21130 0.46 -0.6
Or see this region's nucleotide sequence