Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS17100 and RR42_RS17105 are separated by 152 nucleotides RR42_RS17105 and RR42_RS17110 overlap by 4 nucleotides RR42_RS17110 and RR42_RS17115 are separated by 513 nucleotides
RR42_RS17100: RR42_RS17100 - membrane protein, at 3,704,766 to 3,705,779
_RS17100
RR42_RS17105: RR42_RS17105 - Clp protease ClpX, at 3,705,932 to 3,708,226
_RS17105
RR42_RS17110: RR42_RS17110 - ATP-dependent Clp protease adapter protein ClpS, at 3,708,223 to 3,708,549
_RS17110
RR42_RS17115: RR42_RS17115 - cold-shock protein, at 3,709,063 to 3,709,269
_RS17115
Position (kb)
3705
3706
3707
3708
3709 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3704.992 kb on + strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3704.993 kb on - strand, within RR42_RS17100 at 3705.121 kb on + strand, within RR42_RS17100 at 3705.135 kb on + strand, within RR42_RS17100 at 3705.136 kb on - strand, within RR42_RS17100 at 3705.136 kb on - strand, within RR42_RS17100 at 3705.187 kb on + strand, within RR42_RS17100 at 3705.187 kb on + strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.188 kb on - strand, within RR42_RS17100 at 3705.249 kb on - strand, within RR42_RS17100 at 3705.274 kb on + strand, within RR42_RS17100 at 3705.407 kb on + strand, within RR42_RS17100 at 3705.408 kb on - strand, within RR42_RS17100 at 3705.408 kb on - strand, within RR42_RS17100 at 3705.412 kb on + strand, within RR42_RS17100 at 3705.427 kb on + strand, within RR42_RS17100 at 3705.428 kb on - strand, within RR42_RS17100 at 3705.428 kb on - strand, within RR42_RS17100 at 3705.868 kb on - strand at 3705.883 kb on - strand at 3705.934 kb on - strand at 3705.934 kb on - strand at 3706.459 kb on + strand, within RR42_RS17105 at 3706.460 kb on - strand, within RR42_RS17105 at 3706.498 kb on + strand, within RR42_RS17105 at 3706.498 kb on + strand, within RR42_RS17105 at 3706.508 kb on - strand, within RR42_RS17105 at 3706.605 kb on + strand, within RR42_RS17105 at 3706.605 kb on + strand, within RR42_RS17105 at 3706.605 kb on + strand, within RR42_RS17105 at 3706.606 kb on - strand, within RR42_RS17105 at 3706.653 kb on + strand, within RR42_RS17105 at 3706.653 kb on + strand, within RR42_RS17105 at 3706.654 kb on - strand, within RR42_RS17105 at 3707.106 kb on + strand, within RR42_RS17105 at 3707.107 kb on - strand, within RR42_RS17105 at 3707.107 kb on - strand, within RR42_RS17105 at 3707.244 kb on + strand, within RR42_RS17105 at 3707.448 kb on + strand, within RR42_RS17105 at 3707.488 kb on - strand, within RR42_RS17105 at 3707.488 kb on - strand, within RR42_RS17105 at 3707.488 kb on - strand, within RR42_RS17105 at 3707.856 kb on + strand, within RR42_RS17105 at 3707.856 kb on + strand, within RR42_RS17105 at 3707.856 kb on + strand, within RR42_RS17105 at 3707.856 kb on + strand, within RR42_RS17105 at 3707.856 kb on + strand, within RR42_RS17105 at 3707.857 kb on - strand, within RR42_RS17105 at 3707.857 kb on - strand, within RR42_RS17105 at 3707.857 kb on - strand, within RR42_RS17105 at 3707.857 kb on - strand, within RR42_RS17105 at 3707.857 kb on - strand, within RR42_RS17105 at 3707.859 kb on + strand, within RR42_RS17105 at 3707.859 kb on + strand, within RR42_RS17105 at 3707.859 kb on + strand, within RR42_RS17105 at 3707.859 kb on + strand, within RR42_RS17105 at 3707.860 kb on - strand, within RR42_RS17105 at 3707.860 kb on - strand, within RR42_RS17105 at 3707.860 kb on - strand, within RR42_RS17105 at 3708.076 kb on + strand at 3708.077 kb on - strand at 3708.134 kb on + strand at 3708.152 kb on + strand at 3708.153 kb on - strand at 3708.154 kb on + strand at 3708.154 kb on + strand at 3708.155 kb on - strand at 3708.315 kb on + strand, within RR42_RS17110 at 3708.316 kb on - strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.317 kb on + strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.318 kb on - strand, within RR42_RS17110 at 3708.336 kb on + strand, within RR42_RS17110 at 3708.337 kb on - strand, within RR42_RS17110 at 3708.395 kb on + strand, within RR42_RS17110 at 3708.395 kb on + strand, within RR42_RS17110 at 3708.395 kb on + strand, within RR42_RS17110 at 3708.396 kb on - strand, within RR42_RS17110 at 3708.396 kb on - strand, within RR42_RS17110 at 3708.461 kb on + strand, within RR42_RS17110 at 3708.461 kb on + strand, within RR42_RS17110 at 3708.461 kb on + strand, within RR42_RS17110 at 3708.461 kb on + strand, within RR42_RS17110 at 3708.461 kb on + strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.462 kb on - strand, within RR42_RS17110 at 3708.502 kb on + strand, within RR42_RS17110 at 3708.502 kb on + strand, within RR42_RS17110 at 3708.502 kb on + strand, within RR42_RS17110 at 3708.503 kb on - strand, within RR42_RS17110 at 3708.503 kb on - strand, within RR42_RS17110 at 3708.503 kb on - strand, within RR42_RS17110 at 3708.543 kb on + strand at 3708.543 kb on + strand at 3708.543 kb on + strand at 3708.544 kb on - strand at 3708.555 kb on + strand at 3708.555 kb on + strand at 3708.555 kb on + strand at 3708.568 kb on - strand at 3708.568 kb on - strand at 3708.724 kb on + strand at 3708.724 kb on + strand at 3708.731 kb on + strand at 3708.731 kb on + strand at 3708.731 kb on + strand at 3708.731 kb on + strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.732 kb on - strand at 3708.834 kb on + strand at 3708.995 kb on + strand at 3708.996 kb on - strand at 3709.035 kb on + strand at 3709.035 kb on + strand at 3709.035 kb on + strand at 3709.036 kb on - strand at 3709.036 kb on - strand at 3709.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,704,992 + RR42_RS17100 0.22 +0.4 3,704,993 - RR42_RS17100 0.22 +1.0 3,704,993 - RR42_RS17100 0.22 +0.6 3,704,993 - RR42_RS17100 0.22 -0.4 3,705,121 + RR42_RS17100 0.35 -0.1 3,705,135 + RR42_RS17100 0.36 -1.5 3,705,136 - RR42_RS17100 0.36 +0.5 3,705,136 - RR42_RS17100 0.36 +0.8 3,705,187 + RR42_RS17100 0.42 -0.1 3,705,187 + RR42_RS17100 0.42 +0.5 3,705,188 - RR42_RS17100 0.42 -0.7 3,705,188 - RR42_RS17100 0.42 +1.0 3,705,188 - RR42_RS17100 0.42 -0.2 3,705,188 - RR42_RS17100 0.42 -0.4 3,705,188 - RR42_RS17100 0.42 +1.4 3,705,249 - RR42_RS17100 0.48 +0.5 3,705,274 + RR42_RS17100 0.50 -0.5 3,705,407 + RR42_RS17100 0.63 +0.0 3,705,408 - RR42_RS17100 0.63 -0.5 3,705,408 - RR42_RS17100 0.63 -0.7 3,705,412 + RR42_RS17100 0.64 -0.8 3,705,427 + RR42_RS17100 0.65 -0.2 3,705,428 - RR42_RS17100 0.65 +0.2 3,705,428 - RR42_RS17100 0.65 -0.2 3,705,868 - -0.1 3,705,883 - -0.5 3,705,934 - +0.3 3,705,934 - -0.0 3,706,459 + RR42_RS17105 0.23 -2.7 3,706,460 - RR42_RS17105 0.23 -4.2 3,706,498 + RR42_RS17105 0.25 -2.7 3,706,498 + RR42_RS17105 0.25 -1.5 3,706,508 - RR42_RS17105 0.25 -1.7 3,706,605 + RR42_RS17105 0.29 -1.9 3,706,605 + RR42_RS17105 0.29 -2.9 3,706,605 + RR42_RS17105 0.29 +0.4 3,706,606 - RR42_RS17105 0.29 -2.2 3,706,653 + RR42_RS17105 0.31 -2.0 3,706,653 + RR42_RS17105 0.31 +0.9 3,706,654 - RR42_RS17105 0.31 -3.5 3,707,106 + RR42_RS17105 0.51 -3.5 3,707,107 - RR42_RS17105 0.51 -3.6 3,707,107 - RR42_RS17105 0.51 -3.3 3,707,244 + RR42_RS17105 0.57 -1.9 3,707,448 + RR42_RS17105 0.66 -2.0 3,707,488 - RR42_RS17105 0.68 -2.9 3,707,488 - RR42_RS17105 0.68 -0.1 3,707,488 - RR42_RS17105 0.68 -2.8 3,707,856 + RR42_RS17105 0.84 -2.9 3,707,856 + RR42_RS17105 0.84 -0.1 3,707,856 + RR42_RS17105 0.84 -2.7 3,707,856 + RR42_RS17105 0.84 -2.8 3,707,856 + RR42_RS17105 0.84 -1.5 3,707,857 - RR42_RS17105 0.84 -1.3 3,707,857 - RR42_RS17105 0.84 -2.0 3,707,857 - RR42_RS17105 0.84 -4.0 3,707,857 - RR42_RS17105 0.84 -0.6 3,707,857 - RR42_RS17105 0.84 -3.0 3,707,859 + RR42_RS17105 0.84 -3.7 3,707,859 + RR42_RS17105 0.84 -4.3 3,707,859 + RR42_RS17105 0.84 -0.5 3,707,859 + RR42_RS17105 0.84 -2.1 3,707,860 - RR42_RS17105 0.84 -1.5 3,707,860 - RR42_RS17105 0.84 -3.3 3,707,860 - RR42_RS17105 0.84 -1.1 3,708,076 + -2.5 3,708,077 - -2.6 3,708,134 + -3.1 3,708,152 + -0.9 3,708,153 - -3.5 3,708,154 + -3.1 3,708,154 + -4.2 3,708,155 - -1.1 3,708,315 + RR42_RS17110 0.28 -3.5 3,708,316 - RR42_RS17110 0.28 -0.1 3,708,317 + RR42_RS17110 0.29 -2.2 3,708,317 + RR42_RS17110 0.29 -0.7 3,708,317 + RR42_RS17110 0.29 -3.1 3,708,317 + RR42_RS17110 0.29 +1.9 3,708,317 + RR42_RS17110 0.29 -2.8 3,708,317 + RR42_RS17110 0.29 -3.1 3,708,317 + RR42_RS17110 0.29 -0.7 3,708,317 + RR42_RS17110 0.29 -2.3 3,708,317 + RR42_RS17110 0.29 -0.1 3,708,317 + RR42_RS17110 0.29 -2.0 3,708,317 + RR42_RS17110 0.29 -3.2 3,708,317 + RR42_RS17110 0.29 -2.6 3,708,317 + RR42_RS17110 0.29 -2.3 3,708,318 - RR42_RS17110 0.29 -1.0 3,708,318 - RR42_RS17110 0.29 +0.0 3,708,318 - RR42_RS17110 0.29 -1.5 3,708,318 - RR42_RS17110 0.29 +0.1 3,708,318 - RR42_RS17110 0.29 -0.3 3,708,318 - RR42_RS17110 0.29 +1.5 3,708,318 - RR42_RS17110 0.29 +0.0 3,708,318 - RR42_RS17110 0.29 -0.4 3,708,318 - RR42_RS17110 0.29 -0.8 3,708,318 - RR42_RS17110 0.29 +0.2 3,708,336 + RR42_RS17110 0.35 -2.7 3,708,337 - RR42_RS17110 0.35 -1.6 3,708,395 + RR42_RS17110 0.53 -2.4 3,708,395 + RR42_RS17110 0.53 -3.1 3,708,395 + RR42_RS17110 0.53 -2.1 3,708,396 - RR42_RS17110 0.53 +0.2 3,708,396 - RR42_RS17110 0.53 +0.2 3,708,461 + RR42_RS17110 0.73 -2.3 3,708,461 + RR42_RS17110 0.73 -3.3 3,708,461 + RR42_RS17110 0.73 -1.1 3,708,461 + RR42_RS17110 0.73 -1.6 3,708,461 + RR42_RS17110 0.73 -1.6 3,708,462 - RR42_RS17110 0.73 -1.1 3,708,462 - RR42_RS17110 0.73 +0.2 3,708,462 - RR42_RS17110 0.73 -0.7 3,708,462 - RR42_RS17110 0.73 -0.6 3,708,462 - RR42_RS17110 0.73 +1.4 3,708,462 - RR42_RS17110 0.73 +0.0 3,708,502 + RR42_RS17110 0.85 -1.8 3,708,502 + RR42_RS17110 0.85 -2.5 3,708,502 + RR42_RS17110 0.85 -1.6 3,708,503 - RR42_RS17110 0.86 +0.9 3,708,503 - RR42_RS17110 0.86 +0.3 3,708,503 - RR42_RS17110 0.86 -0.6 3,708,543 + -2.7 3,708,543 + -0.1 3,708,543 + -1.4 3,708,544 - +0.0 3,708,555 + -2.1 3,708,555 + -1.7 3,708,555 + -2.2 3,708,568 - -0.0 3,708,568 - -0.2 3,708,724 + +0.4 3,708,724 + -0.2 3,708,731 + +0.3 3,708,731 + -0.3 3,708,731 + -0.4 3,708,731 + +0.3 3,708,732 - +0.1 3,708,732 - +0.3 3,708,732 - +0.0 3,708,732 - +0.6 3,708,732 - +0.9 3,708,732 - +0.7 3,708,732 - +0.3 3,708,732 - -0.3 3,708,834 + -0.0 3,708,995 + -1.1 3,708,996 - +0.6 3,709,035 + +0.6 3,709,035 + +0.1 3,709,035 + -0.3 3,709,036 - +0.4 3,709,036 - +0.7 3,709,063 - -1.1
Or see this region's nucleotide sequence