Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15270 and RR42_RS15275 are separated by 232 nucleotides RR42_RS15275 and RR42_RS15280 are separated by 57 nucleotides
RR42_RS15270: RR42_RS15270 - gamma-glutamyl cyclotransferase, at 3,303,806 to 3,304,510
_RS15270
RR42_RS15275: RR42_RS15275 - ankyrin, at 3,304,743 to 3,305,330
_RS15275
RR42_RS15280: RR42_RS15280 - long-chain fatty acid--CoA ligase, at 3,305,388 to 3,307,103
_RS15280
Position (kb)
3304
3305
3306 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3303.795 kb on - strand at 3303.883 kb on - strand, within RR42_RS15270 at 3303.977 kb on + strand, within RR42_RS15270 at 3304.008 kb on + strand, within RR42_RS15270 at 3304.008 kb on + strand, within RR42_RS15270 at 3304.008 kb on + strand, within RR42_RS15270 at 3304.009 kb on - strand, within RR42_RS15270 at 3304.058 kb on + strand, within RR42_RS15270 at 3304.058 kb on + strand, within RR42_RS15270 at 3304.058 kb on + strand, within RR42_RS15270 at 3304.058 kb on + strand, within RR42_RS15270 at 3304.058 kb on + strand, within RR42_RS15270 at 3304.059 kb on - strand, within RR42_RS15270 at 3304.059 kb on - strand, within RR42_RS15270 at 3304.059 kb on - strand, within RR42_RS15270 at 3304.059 kb on - strand, within RR42_RS15270 at 3304.221 kb on - strand, within RR42_RS15270 at 3304.221 kb on - strand, within RR42_RS15270 at 3304.307 kb on + strand, within RR42_RS15270 at 3304.307 kb on + strand, within RR42_RS15270 at 3304.308 kb on - strand, within RR42_RS15270 at 3304.367 kb on + strand, within RR42_RS15270 at 3304.368 kb on - strand, within RR42_RS15270 at 3304.385 kb on + strand, within RR42_RS15270 at 3304.727 kb on + strand at 3304.866 kb on + strand, within RR42_RS15275 at 3304.866 kb on + strand, within RR42_RS15275 at 3304.878 kb on - strand, within RR42_RS15275 at 3305.034 kb on + strand, within RR42_RS15275 at 3305.035 kb on - strand, within RR42_RS15275 at 3305.123 kb on + strand, within RR42_RS15275 at 3305.286 kb on + strand at 3305.287 kb on - strand at 3305.287 kb on - strand at 3305.287 kb on - strand at 3305.506 kb on + strand at 3305.507 kb on - strand at 3305.507 kb on - strand at 3305.507 kb on - strand at 3305.512 kb on + strand at 3305.512 kb on + strand at 3305.512 kb on + strand at 3305.513 kb on - strand at 3305.668 kb on + strand, within RR42_RS15280 at 3305.669 kb on - strand, within RR42_RS15280 at 3305.669 kb on - strand, within RR42_RS15280 at 3305.669 kb on - strand, within RR42_RS15280 at 3305.749 kb on + strand, within RR42_RS15280 at 3305.749 kb on + strand, within RR42_RS15280 at 3305.749 kb on + strand, within RR42_RS15280 at 3305.749 kb on + strand, within RR42_RS15280 at 3305.750 kb on - strand, within RR42_RS15280 at 3305.750 kb on - strand, within RR42_RS15280 at 3305.794 kb on + strand, within RR42_RS15280 at 3305.794 kb on + strand, within RR42_RS15280 at 3305.948 kb on + strand, within RR42_RS15280 at 3305.949 kb on - strand, within RR42_RS15280 at 3306.035 kb on - strand, within RR42_RS15280 at 3306.035 kb on - strand, within RR42_RS15280 at 3306.035 kb on - strand, within RR42_RS15280 at 3306.046 kb on + strand, within RR42_RS15280 at 3306.047 kb on - strand, within RR42_RS15280 at 3306.047 kb on - strand, within RR42_RS15280 at 3306.050 kb on + strand, within RR42_RS15280 at 3306.118 kb on + strand, within RR42_RS15280 at 3306.119 kb on - strand, within RR42_RS15280 at 3306.188 kb on + strand, within RR42_RS15280 at 3306.270 kb on - strand, within RR42_RS15280 at 3306.301 kb on + strand, within RR42_RS15280
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,303,795 - +0.1 3,303,883 - RR42_RS15270 0.11 +0.3 3,303,977 + RR42_RS15270 0.24 -0.1 3,304,008 + RR42_RS15270 0.29 -0.1 3,304,008 + RR42_RS15270 0.29 -0.7 3,304,008 + RR42_RS15270 0.29 -0.8 3,304,009 - RR42_RS15270 0.29 -1.6 3,304,058 + RR42_RS15270 0.36 -0.2 3,304,058 + RR42_RS15270 0.36 -0.6 3,304,058 + RR42_RS15270 0.36 -0.9 3,304,058 + RR42_RS15270 0.36 -2.8 3,304,058 + RR42_RS15270 0.36 +0.7 3,304,059 - RR42_RS15270 0.36 +0.8 3,304,059 - RR42_RS15270 0.36 -0.0 3,304,059 - RR42_RS15270 0.36 -2.3 3,304,059 - RR42_RS15270 0.36 -0.7 3,304,221 - RR42_RS15270 0.59 -0.3 3,304,221 - RR42_RS15270 0.59 +0.3 3,304,307 + RR42_RS15270 0.71 +0.1 3,304,307 + RR42_RS15270 0.71 -0.1 3,304,308 - RR42_RS15270 0.71 +0.1 3,304,367 + RR42_RS15270 0.80 +1.7 3,304,368 - RR42_RS15270 0.80 -0.8 3,304,385 + RR42_RS15270 0.82 +0.0 3,304,727 + +0.2 3,304,866 + RR42_RS15275 0.21 -0.4 3,304,866 + RR42_RS15275 0.21 -1.3 3,304,878 - RR42_RS15275 0.23 +2.3 3,305,034 + RR42_RS15275 0.49 -0.3 3,305,035 - RR42_RS15275 0.50 -0.4 3,305,123 + RR42_RS15275 0.65 -1.0 3,305,286 + -0.0 3,305,287 - -0.3 3,305,287 - +1.2 3,305,287 - -0.5 3,305,506 + -0.4 3,305,507 - +0.1 3,305,507 - +0.3 3,305,507 - -1.5 3,305,512 + +1.3 3,305,512 + +0.1 3,305,512 + +0.8 3,305,513 - -1.2 3,305,668 + RR42_RS15280 0.16 +0.3 3,305,669 - RR42_RS15280 0.16 +1.5 3,305,669 - RR42_RS15280 0.16 -0.4 3,305,669 - RR42_RS15280 0.16 +0.2 3,305,749 + RR42_RS15280 0.21 +0.5 3,305,749 + RR42_RS15280 0.21 -1.6 3,305,749 + RR42_RS15280 0.21 +0.3 3,305,749 + RR42_RS15280 0.21 +0.7 3,305,750 - RR42_RS15280 0.21 -1.3 3,305,750 - RR42_RS15280 0.21 +0.9 3,305,794 + RR42_RS15280 0.24 -0.1 3,305,794 + RR42_RS15280 0.24 +0.0 3,305,948 + RR42_RS15280 0.33 +0.0 3,305,949 - RR42_RS15280 0.33 +0.4 3,306,035 - RR42_RS15280 0.38 +2.5 3,306,035 - RR42_RS15280 0.38 +0.0 3,306,035 - RR42_RS15280 0.38 -0.3 3,306,046 + RR42_RS15280 0.38 -0.4 3,306,047 - RR42_RS15280 0.38 +0.2 3,306,047 - RR42_RS15280 0.38 -0.6 3,306,050 + RR42_RS15280 0.39 +0.3 3,306,118 + RR42_RS15280 0.43 +0.2 3,306,119 - RR42_RS15280 0.43 -0.2 3,306,188 + RR42_RS15280 0.47 +0.3 3,306,270 - RR42_RS15280 0.51 +0.2 3,306,301 + RR42_RS15280 0.53 -1.6
Or see this region's nucleotide sequence