Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS14080 and RR42_RS14085 are separated by 129 nucleotides RR42_RS14085 and RR42_RS14090 are separated by 64 nucleotides RR42_RS14090 and RR42_RS14095 are separated by 199 nucleotides RR42_RS14095 and RR42_RS14100 are separated by 170 nucleotides
RR42_RS14080: RR42_RS14080 - RNA polymerase sigma factor RpoE, at 3,030,362 to 3,030,961
_RS14080
RR42_RS14085: RR42_RS14085 - hypothetical protein, at 3,031,091 to 3,031,546
_RS14085
RR42_RS14090: RR42_RS14090 - 3-oxoacyl-ACP synthase, at 3,031,611 to 3,032,843
_RS14090
RR42_RS14095: RR42_RS14095 - acyl carrier protein, at 3,033,043 to 3,033,282
_RS14095
RR42_RS14100: RR42_RS14100 - 3-ketoacyl-ACP reductase, at 3,033,453 to 3,034,202
_RS14100
Position (kb)
3031
3032
3033 Strain fitness (log2 ratio)
-1
0
1
2 at 3030.613 kb on + strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.614 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.621 kb on - strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.705 kb on + strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.706 kb on - strand, within RR42_RS14080 at 3030.747 kb on + strand, within RR42_RS14080 at 3030.786 kb on + strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.787 kb on - strand, within RR42_RS14080 at 3030.876 kb on + strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.877 kb on - strand, within RR42_RS14080 at 3030.966 kb on - strand at 3030.966 kb on - strand at 3030.981 kb on + strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3030.982 kb on - strand at 3031.014 kb on + strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.024 kb on - strand at 3031.060 kb on + strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.061 kb on - strand at 3031.081 kb on + strand at 3031.082 kb on - strand at 3031.138 kb on + strand, within RR42_RS14085 at 3031.139 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.304 kb on - strand, within RR42_RS14085 at 3031.359 kb on + strand, within RR42_RS14085 at 3031.360 kb on - strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3031.363 kb on + strand, within RR42_RS14085 at 3033.360 kb on - strand at 3033.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,030,613 + RR42_RS14080 0.42 +0.6 3,030,614 - RR42_RS14080 0.42 -1.5 3,030,614 - RR42_RS14080 0.42 +0.3 3,030,621 - RR42_RS14080 0.43 -0.3 3,030,621 - RR42_RS14080 0.43 -0.1 3,030,705 + RR42_RS14080 0.57 -0.1 3,030,705 + RR42_RS14080 0.57 +0.2 3,030,705 + RR42_RS14080 0.57 +0.7 3,030,705 + RR42_RS14080 0.57 -0.1 3,030,706 - RR42_RS14080 0.57 -0.5 3,030,706 - RR42_RS14080 0.57 -0.1 3,030,747 + RR42_RS14080 0.64 -0.2 3,030,786 + RR42_RS14080 0.71 -0.2 3,030,787 - RR42_RS14080 0.71 -0.5 3,030,787 - RR42_RS14080 0.71 +0.1 3,030,876 + RR42_RS14080 0.86 +0.1 3,030,877 - RR42_RS14080 0.86 -0.5 3,030,877 - RR42_RS14080 0.86 -0.9 3,030,966 - -1.3 3,030,966 - -0.9 3,030,981 + -0.1 3,030,982 - -0.6 3,030,982 - -0.7 3,030,982 - +0.3 3,031,014 + -1.6 3,031,024 - +0.3 3,031,024 - -0.6 3,031,024 - +0.2 3,031,024 - -0.3 3,031,060 + +0.2 3,031,061 - -0.8 3,031,061 - -0.5 3,031,061 - -0.5 3,031,081 + -0.1 3,031,082 - +0.3 3,031,138 + RR42_RS14085 0.10 -0.5 3,031,139 - RR42_RS14085 0.11 +0.1 3,031,304 - RR42_RS14085 0.47 +0.4 3,031,304 - RR42_RS14085 0.47 -0.7 3,031,304 - RR42_RS14085 0.47 +0.2 3,031,304 - RR42_RS14085 0.47 -0.4 3,031,359 + RR42_RS14085 0.59 -0.9 3,031,360 - RR42_RS14085 0.59 +1.2 3,031,363 + RR42_RS14085 0.60 -0.3 3,031,363 + RR42_RS14085 0.60 -0.3 3,031,363 + RR42_RS14085 0.60 +2.2 3,033,360 - +2.2 3,033,372 - +0.7
Or see this region's nucleotide sequence