Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS13545

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS13540 and RR42_RS13545 are separated by 15 nucleotidesRR42_RS13545 and RR42_RS13550 are separated by 52 nucleotidesRR42_RS13550 and RR42_RS13555 are separated by 2 nucleotides RR42_RS13540: RR42_RS13540 - acyl-CoA dehydrogenase, at 2,922,637 to 2,923,800 _RS13540 RR42_RS13545: RR42_RS13545 - enoyl-CoA hydratase, at 2,923,816 to 2,924,667 _RS13545 RR42_RS13550: RR42_RS13550 - 3-hydroxyacyl-CoA dehydrogenase, at 2,924,720 to 2,925,520 _RS13550 RR42_RS13555: RR42_RS13555 - anthraniloyl-CoA monooxygenase (EC 1.14.13.40) (from data), at 2,925,523 to 2,927,913 _RS13555 Position (kb) 2923 2924 2925Strain fitness (log2 ratio) -1 0 1at 2922.893 kb on + strand, within RR42_RS13540at 2923.289 kb on + strand, within RR42_RS13540at 2923.290 kb on - strand, within RR42_RS13540at 2923.808 kb on + strandat 2923.809 kb on - strandat 2923.809 kb on - strandat 2923.809 kb on - strandat 2923.809 kb on - strandat 2923.863 kb on - strandat 2924.656 kb on + strandat 2924.767 kb on + strandat 2924.942 kb on - strand, within RR42_RS13550at 2925.051 kb on + strand, within RR42_RS13550at 2925.051 kb on + strand, within RR42_RS13550at 2925.052 kb on - strand, within RR42_RS13550at 2925.058 kb on - strand, within RR42_RS13550at 2925.091 kb on + strand, within RR42_RS13550at 2925.092 kb on - strand, within RR42_RS13550at 2925.608 kb on + strandat 2925.609 kb on - strandat 2925.609 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,922,893 + RR42_RS13540 0.22 +0.1
2,923,289 + RR42_RS13540 0.56 -0.6
2,923,290 - RR42_RS13540 0.56 +0.7
2,923,808 + -0.2
2,923,809 - -0.5
2,923,809 - +0.9
2,923,809 - +0.9
2,923,809 - -0.0
2,923,863 - -0.6
2,924,656 + -1.2
2,924,767 + -0.3
2,924,942 - RR42_RS13550 0.28 -0.1
2,925,051 + RR42_RS13550 0.41 +0.2
2,925,051 + RR42_RS13550 0.41 -0.1
2,925,052 - RR42_RS13550 0.41 -1.2
2,925,058 - RR42_RS13550 0.42 -0.4
2,925,091 + RR42_RS13550 0.46 -0.5
2,925,092 - RR42_RS13550 0.46 -0.3
2,925,608 + +0.7
2,925,609 - +0.7
2,925,609 - +0.7

Or see this region's nucleotide sequence