Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS12495

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS12485 and RR42_RS12490 overlap by 8 nucleotidesRR42_RS12490 and RR42_RS12495 are separated by 50 nucleotidesRR42_RS12495 and RR42_RS12500 are separated by 112 nucleotidesRR42_RS12500 and RR42_RS12505 overlap by 4 nucleotides RR42_RS12485: RR42_RS12485 - ABC transporter ATP-binding protein, at 2,685,743 to 2,686,489 _RS12485 RR42_RS12490: RR42_RS12490 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,686,482 to 2,687,276 _RS12490 RR42_RS12495: RR42_RS12495 - ABC transporter permease, at 2,687,327 to 2,688,511 _RS12495 RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403 _RS12500 RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005 _RS12505 Position (kb) 2687 2688 2689Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2686.537 kb on + strandat 2686.537 kb on + strandat 2686.989 kb on + strand, within RR42_RS12490at 2686.989 kb on + strand, within RR42_RS12490at 2686.990 kb on - strand, within RR42_RS12490at 2687.134 kb on + strand, within RR42_RS12490at 2687.135 kb on - strand, within RR42_RS12490at 2687.135 kb on - strand, within RR42_RS12490at 2687.135 kb on - strand, within RR42_RS12490at 2687.301 kb on - strandat 2687.301 kb on - strandat 2687.301 kb on - strandat 2687.433 kb on + strandat 2687.433 kb on + strandat 2687.434 kb on - strandat 2687.634 kb on + strand, within RR42_RS12495at 2687.634 kb on + strand, within RR42_RS12495at 2687.685 kb on + strand, within RR42_RS12495at 2687.686 kb on - strand, within RR42_RS12495at 2687.691 kb on + strand, within RR42_RS12495at 2687.692 kb on - strand, within RR42_RS12495at 2687.692 kb on - strand, within RR42_RS12495at 2687.692 kb on - strand, within RR42_RS12495at 2687.692 kb on - strand, within RR42_RS12495at 2687.724 kb on + strand, within RR42_RS12495at 2687.775 kb on + strand, within RR42_RS12495at 2687.922 kb on + strand, within RR42_RS12495at 2687.922 kb on + strand, within RR42_RS12495at 2687.922 kb on + strand, within RR42_RS12495at 2687.923 kb on - strand, within RR42_RS12495at 2687.994 kb on + strand, within RR42_RS12495at 2687.994 kb on + strand, within RR42_RS12495at 2688.093 kb on + strand, within RR42_RS12495at 2688.093 kb on + strand, within RR42_RS12495at 2688.094 kb on - strand, within RR42_RS12495at 2688.094 kb on - strand, within RR42_RS12495at 2688.241 kb on - strand, within RR42_RS12495at 2688.345 kb on + strand, within RR42_RS12495at 2688.414 kb on + strandat 2688.415 kb on - strandat 2688.415 kb on - strandat 2688.626 kb on - strandat 2688.626 kb on - strandat 2688.626 kb on - strandat 2688.991 kb on + strand, within RR42_RS12500at 2688.991 kb on + strand, within RR42_RS12500at 2688.991 kb on + strand, within RR42_RS12500at 2688.992 kb on - strand, within RR42_RS12500at 2688.992 kb on - strand, within RR42_RS12500at 2688.994 kb on + strand, within RR42_RS12500at 2688.994 kb on + strand, within RR42_RS12500at 2688.995 kb on - strand, within RR42_RS12500at 2689.441 kb on + strandat 2689.486 kb on - strand, within RR42_RS12505

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,686,537 + +0.5
2,686,537 + +1.2
2,686,989 + RR42_RS12490 0.64 -0.4
2,686,989 + RR42_RS12490 0.64 +0.0
2,686,990 - RR42_RS12490 0.64 -0.6
2,687,134 + RR42_RS12490 0.82 -1.5
2,687,135 - RR42_RS12490 0.82 -0.6
2,687,135 - RR42_RS12490 0.82 +1.3
2,687,135 - RR42_RS12490 0.82 -0.4
2,687,301 - -1.7
2,687,301 - +0.2
2,687,301 - +1.1
2,687,433 + -1.7
2,687,433 + -0.7
2,687,434 - -1.7
2,687,634 + RR42_RS12495 0.26 +0.7
2,687,634 + RR42_RS12495 0.26 +0.1
2,687,685 + RR42_RS12495 0.30 +0.3
2,687,686 - RR42_RS12495 0.30 -0.0
2,687,691 + RR42_RS12495 0.31 +0.4
2,687,692 - RR42_RS12495 0.31 +0.7
2,687,692 - RR42_RS12495 0.31 +0.1
2,687,692 - RR42_RS12495 0.31 +0.2
2,687,692 - RR42_RS12495 0.31 +0.4
2,687,724 + RR42_RS12495 0.34 -2.9
2,687,775 + RR42_RS12495 0.38 +0.3
2,687,922 + RR42_RS12495 0.50 +1.9
2,687,922 + RR42_RS12495 0.50 +0.3
2,687,922 + RR42_RS12495 0.50 -0.0
2,687,923 - RR42_RS12495 0.50 +0.4
2,687,994 + RR42_RS12495 0.56 +0.4
2,687,994 + RR42_RS12495 0.56 +0.5
2,688,093 + RR42_RS12495 0.65 +1.4
2,688,093 + RR42_RS12495 0.65 +0.6
2,688,094 - RR42_RS12495 0.65 +1.2
2,688,094 - RR42_RS12495 0.65 +0.6
2,688,241 - RR42_RS12495 0.77 +1.7
2,688,345 + RR42_RS12495 0.86 +0.0
2,688,414 + -0.5
2,688,415 - +0.2
2,688,415 - +0.3
2,688,626 - +0.5
2,688,626 - +0.2
2,688,626 - -2.9
2,688,991 + RR42_RS12500 0.47 +0.5
2,688,991 + RR42_RS12500 0.47 +0.6
2,688,991 + RR42_RS12500 0.47 +0.1
2,688,992 - RR42_RS12500 0.47 +0.2
2,688,992 - RR42_RS12500 0.47 -0.8
2,688,994 + RR42_RS12500 0.47 +0.3
2,688,994 + RR42_RS12500 0.47 -0.3
2,688,995 - RR42_RS12500 0.48 -0.4
2,689,441 + +0.0
2,689,486 - RR42_RS12505 0.14 -0.6

Or see this region's nucleotide sequence