Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12485 and RR42_RS12490 overlap by 8 nucleotides RR42_RS12490 and RR42_RS12495 are separated by 50 nucleotides RR42_RS12495 and RR42_RS12500 are separated by 112 nucleotides RR42_RS12500 and RR42_RS12505 overlap by 4 nucleotides
RR42_RS12485: RR42_RS12485 - ABC transporter ATP-binding protein, at 2,685,743 to 2,686,489
_RS12485
RR42_RS12490: RR42_RS12490 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,686,482 to 2,687,276
_RS12490
RR42_RS12495: RR42_RS12495 - ABC transporter permease, at 2,687,327 to 2,688,511
_RS12495
RR42_RS12500: RR42_RS12500 - enoyl-CoA hydratase, at 2,688,624 to 2,689,403
_RS12500
RR42_RS12505: RR42_RS12505 - TetR family transcriptional regulator, at 2,689,400 to 2,690,005
_RS12505
Position (kb)
2687
2688
2689 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2686.537 kb on + strand at 2686.537 kb on + strand at 2686.989 kb on + strand, within RR42_RS12490 at 2686.989 kb on + strand, within RR42_RS12490 at 2686.990 kb on - strand, within RR42_RS12490 at 2687.134 kb on + strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.135 kb on - strand, within RR42_RS12490 at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.301 kb on - strand at 2687.433 kb on + strand at 2687.433 kb on + strand at 2687.434 kb on - strand at 2687.634 kb on + strand, within RR42_RS12495 at 2687.634 kb on + strand, within RR42_RS12495 at 2687.685 kb on + strand, within RR42_RS12495 at 2687.686 kb on - strand, within RR42_RS12495 at 2687.691 kb on + strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.692 kb on - strand, within RR42_RS12495 at 2687.724 kb on + strand, within RR42_RS12495 at 2687.775 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.922 kb on + strand, within RR42_RS12495 at 2687.923 kb on - strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2687.994 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.093 kb on + strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.094 kb on - strand, within RR42_RS12495 at 2688.241 kb on - strand, within RR42_RS12495 at 2688.345 kb on + strand, within RR42_RS12495 at 2688.414 kb on + strand at 2688.415 kb on - strand at 2688.415 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.626 kb on - strand at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.991 kb on + strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.992 kb on - strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.994 kb on + strand, within RR42_RS12500 at 2688.995 kb on - strand, within RR42_RS12500 at 2689.441 kb on + strand at 2689.486 kb on - strand, within RR42_RS12505
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,686,537 + +0.5 2,686,537 + +1.2 2,686,989 + RR42_RS12490 0.64 -0.4 2,686,989 + RR42_RS12490 0.64 +0.0 2,686,990 - RR42_RS12490 0.64 -0.6 2,687,134 + RR42_RS12490 0.82 -1.5 2,687,135 - RR42_RS12490 0.82 -0.6 2,687,135 - RR42_RS12490 0.82 +1.3 2,687,135 - RR42_RS12490 0.82 -0.4 2,687,301 - -1.7 2,687,301 - +0.2 2,687,301 - +1.1 2,687,433 + -1.7 2,687,433 + -0.7 2,687,434 - -1.7 2,687,634 + RR42_RS12495 0.26 +0.7 2,687,634 + RR42_RS12495 0.26 +0.1 2,687,685 + RR42_RS12495 0.30 +0.3 2,687,686 - RR42_RS12495 0.30 -0.0 2,687,691 + RR42_RS12495 0.31 +0.4 2,687,692 - RR42_RS12495 0.31 +0.7 2,687,692 - RR42_RS12495 0.31 +0.1 2,687,692 - RR42_RS12495 0.31 +0.2 2,687,692 - RR42_RS12495 0.31 +0.4 2,687,724 + RR42_RS12495 0.34 -2.9 2,687,775 + RR42_RS12495 0.38 +0.3 2,687,922 + RR42_RS12495 0.50 +1.9 2,687,922 + RR42_RS12495 0.50 +0.3 2,687,922 + RR42_RS12495 0.50 -0.0 2,687,923 - RR42_RS12495 0.50 +0.4 2,687,994 + RR42_RS12495 0.56 +0.4 2,687,994 + RR42_RS12495 0.56 +0.5 2,688,093 + RR42_RS12495 0.65 +1.4 2,688,093 + RR42_RS12495 0.65 +0.6 2,688,094 - RR42_RS12495 0.65 +1.2 2,688,094 - RR42_RS12495 0.65 +0.6 2,688,241 - RR42_RS12495 0.77 +1.7 2,688,345 + RR42_RS12495 0.86 +0.0 2,688,414 + -0.5 2,688,415 - +0.2 2,688,415 - +0.3 2,688,626 - +0.5 2,688,626 - +0.2 2,688,626 - -2.9 2,688,991 + RR42_RS12500 0.47 +0.5 2,688,991 + RR42_RS12500 0.47 +0.6 2,688,991 + RR42_RS12500 0.47 +0.1 2,688,992 - RR42_RS12500 0.47 +0.2 2,688,992 - RR42_RS12500 0.47 -0.8 2,688,994 + RR42_RS12500 0.47 +0.3 2,688,994 + RR42_RS12500 0.47 -0.3 2,688,995 - RR42_RS12500 0.48 -0.4 2,689,441 + +0.0 2,689,486 - RR42_RS12505 0.14 -0.6
Or see this region's nucleotide sequence