Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS12415 and RR42_RS12420 are separated by 69 nucleotides RR42_RS12420 and RR42_RS12425 overlap by 11 nucleotides
RR42_RS12415: RR42_RS12415 - ABC transporter substrate-binding protein, at 2,665,735 to 2,666,724
_RS12415
RR42_RS12420: RR42_RS12420 - CoA-transferase, at 2,666,794 to 2,667,948
_RS12420
RR42_RS12425: RR42_RS12425 - 3-hydroxy-3-methylglutaryl-CoA lyase, at 2,667,938 to 2,668,918
_RS12425
Position (kb)
2666
2667
2668 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2665.942 kb on + strand, within RR42_RS12415 at 2665.943 kb on - strand, within RR42_RS12415 at 2665.970 kb on + strand, within RR42_RS12415 at 2665.970 kb on + strand, within RR42_RS12415 at 2665.970 kb on + strand, within RR42_RS12415 at 2665.970 kb on + strand, within RR42_RS12415 at 2666.156 kb on + strand, within RR42_RS12415 at 2666.157 kb on - strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.243 kb on + strand, within RR42_RS12415 at 2666.244 kb on - strand, within RR42_RS12415 at 2666.244 kb on - strand, within RR42_RS12415 at 2666.244 kb on - strand, within RR42_RS12415 at 2666.396 kb on + strand, within RR42_RS12415 at 2666.396 kb on + strand, within RR42_RS12415 at 2666.664 kb on + strand at 2666.664 kb on + strand at 2666.665 kb on - strand at 2666.665 kb on - strand at 2666.689 kb on - strand at 2666.736 kb on + strand at 2666.737 kb on - strand at 2666.737 kb on - strand at 2666.737 kb on - strand at 2666.737 kb on - strand at 2666.743 kb on - strand at 2666.908 kb on + strand at 2666.908 kb on + strand at 2667.126 kb on + strand, within RR42_RS12420 at 2667.321 kb on + strand, within RR42_RS12420 at 2667.322 kb on - strand, within RR42_RS12420 at 2667.322 kb on - strand, within RR42_RS12420 at 2667.500 kb on + strand, within RR42_RS12420 at 2667.501 kb on - strand, within RR42_RS12420 at 2667.501 kb on - strand, within RR42_RS12420 at 2667.554 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.587 kb on + strand, within RR42_RS12420 at 2667.588 kb on - strand, within RR42_RS12420 at 2667.618 kb on + strand, within RR42_RS12420 at 2667.618 kb on + strand, within RR42_RS12420 at 2667.624 kb on - strand, within RR42_RS12420 at 2667.624 kb on - strand, within RR42_RS12420 at 2667.889 kb on - strand at 2668.128 kb on + strand, within RR42_RS12425
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,665,942 + RR42_RS12415 0.21 -2.6 2,665,943 - RR42_RS12415 0.21 -0.4 2,665,970 + RR42_RS12415 0.24 -0.0 2,665,970 + RR42_RS12415 0.24 +0.5 2,665,970 + RR42_RS12415 0.24 -0.8 2,665,970 + RR42_RS12415 0.24 -0.3 2,666,156 + RR42_RS12415 0.43 +0.1 2,666,157 - RR42_RS12415 0.43 +0.1 2,666,243 + RR42_RS12415 0.51 +0.9 2,666,243 + RR42_RS12415 0.51 -0.2 2,666,243 + RR42_RS12415 0.51 +1.0 2,666,243 + RR42_RS12415 0.51 +1.9 2,666,243 + RR42_RS12415 0.51 -0.9 2,666,243 + RR42_RS12415 0.51 +0.0 2,666,244 - RR42_RS12415 0.51 +0.6 2,666,244 - RR42_RS12415 0.51 +1.0 2,666,244 - RR42_RS12415 0.51 +1.4 2,666,396 + RR42_RS12415 0.67 -0.3 2,666,396 + RR42_RS12415 0.67 +1.4 2,666,664 + -0.0 2,666,664 + +1.3 2,666,665 - +0.2 2,666,665 - +0.2 2,666,689 - +0.3 2,666,736 + -0.0 2,666,737 - +0.3 2,666,737 - -0.5 2,666,737 - +0.2 2,666,737 - +0.1 2,666,743 - -0.2 2,666,908 + +0.4 2,666,908 + +0.1 2,667,126 + RR42_RS12420 0.29 +0.6 2,667,321 + RR42_RS12420 0.46 -1.8 2,667,322 - RR42_RS12420 0.46 +0.2 2,667,322 - RR42_RS12420 0.46 +0.3 2,667,500 + RR42_RS12420 0.61 -0.6 2,667,501 - RR42_RS12420 0.61 -0.0 2,667,501 - RR42_RS12420 0.61 +0.2 2,667,554 + RR42_RS12420 0.66 -0.0 2,667,587 + RR42_RS12420 0.69 +0.4 2,667,587 + RR42_RS12420 0.69 +1.4 2,667,587 + RR42_RS12420 0.69 +0.8 2,667,587 + RR42_RS12420 0.69 +0.1 2,667,587 + RR42_RS12420 0.69 +0.2 2,667,587 + RR42_RS12420 0.69 -1.0 2,667,587 + RR42_RS12420 0.69 -0.4 2,667,588 - RR42_RS12420 0.69 -0.2 2,667,618 + RR42_RS12420 0.71 -0.2 2,667,618 + RR42_RS12420 0.71 +0.0 2,667,624 - RR42_RS12420 0.72 -0.3 2,667,624 - RR42_RS12420 0.72 -0.9 2,667,889 - +0.5 2,668,128 + RR42_RS12425 0.19 +0.1
Or see this region's nucleotide sequence