Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS09545

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS09535 and RR42_RS09540 are separated by 260 nucleotidesRR42_RS09540 and RR42_RS09545 are separated by 34 nucleotidesRR42_RS09545 and RR42_RS09550 are separated by 109 nucleotidesRR42_RS09550 and RR42_RS09555 are separated by 276 nucleotides RR42_RS09535: RR42_RS09535 - AraC family transcriptional regulator, at 2,020,976 to 2,022,025 _RS09535 RR42_RS09540: RR42_RS09540 - twin-arginine translocation pathway signal protein, at 2,022,286 to 2,022,846 _RS09540 RR42_RS09545: RR42_RS09545 - peptidase, at 2,022,881 to 2,024,464 _RS09545 RR42_RS09550: RR42_RS09550 - universal stress protein UspA, at 2,024,574 to 2,025,029 _RS09550 RR42_RS09555: RR42_RS09555 - universal stress protein UspA, at 2,025,306 to 2,025,806 _RS09555 Position (kb) 2022 2023 2024 2025Strain fitness (log2 ratio) -1 0 1 2at 2021.914 kb on - strand, within RR42_RS09535at 2022.170 kb on - strandat 2022.185 kb on + strandat 2022.185 kb on + strandat 2022.287 kb on + strandat 2022.338 kb on + strandat 2022.339 kb on - strandat 2022.339 kb on - strandat 2022.339 kb on - strandat 2022.435 kb on + strand, within RR42_RS09540at 2022.435 kb on + strand, within RR42_RS09540at 2022.436 kb on - strand, within RR42_RS09540at 2022.445 kb on - strand, within RR42_RS09540at 2022.445 kb on - strand, within RR42_RS09540at 2022.575 kb on - strand, within RR42_RS09540at 2022.674 kb on + strand, within RR42_RS09540at 2022.675 kb on - strand, within RR42_RS09540at 2022.882 kb on + strandat 2022.883 kb on - strandat 2022.883 kb on - strandat 2022.969 kb on + strandat 2022.969 kb on + strandat 2022.970 kb on - strandat 2022.970 kb on - strandat 2022.970 kb on - strandat 2022.973 kb on + strandat 2023.024 kb on - strandat 2023.233 kb on + strand, within RR42_RS09545at 2023.233 kb on + strand, within RR42_RS09545at 2023.234 kb on - strand, within RR42_RS09545at 2023.234 kb on - strand, within RR42_RS09545at 2023.305 kb on - strand, within RR42_RS09545at 2023.305 kb on - strand, within RR42_RS09545at 2023.473 kb on + strand, within RR42_RS09545at 2023.526 kb on - strand, within RR42_RS09545at 2023.812 kb on + strand, within RR42_RS09545at 2023.813 kb on - strand, within RR42_RS09545at 2023.813 kb on - strand, within RR42_RS09545at 2023.890 kb on + strand, within RR42_RS09545at 2023.891 kb on - strand, within RR42_RS09545at 2024.166 kb on + strand, within RR42_RS09545at 2024.167 kb on - strand, within RR42_RS09545at 2024.878 kb on + strand, within RR42_RS09550at 2024.878 kb on + strand, within RR42_RS09550at 2024.878 kb on + strand, within RR42_RS09550at 2024.878 kb on + strand, within RR42_RS09550at 2024.878 kb on + strand, within RR42_RS09550at 2024.878 kb on + strand, within RR42_RS09550at 2024.879 kb on - strand, within RR42_RS09550at 2024.900 kb on + strand, within RR42_RS09550at 2024.920 kb on + strand, within RR42_RS09550at 2024.920 kb on + strand, within RR42_RS09550at 2024.920 kb on + strand, within RR42_RS09550at 2024.931 kb on + strand, within RR42_RS09550at 2024.932 kb on - strand, within RR42_RS09550at 2024.932 kb on - strand, within RR42_RS09550at 2024.980 kb on - strand, within RR42_RS09550at 2024.980 kb on - strand, within RR42_RS09550at 2025.016 kb on - strandat 2025.016 kb on - strandat 2025.025 kb on + strandat 2025.025 kb on + strandat 2025.025 kb on + strandat 2025.073 kb on + strandat 2025.111 kb on + strandat 2025.112 kb on - strandat 2025.112 kb on - strandat 2025.112 kb on - strandat 2025.132 kb on + strandat 2025.132 kb on + strandat 2025.133 kb on - strandat 2025.133 kb on - strandat 2025.133 kb on - strandat 2025.218 kb on - strandat 2025.244 kb on + strandat 2025.300 kb on + strandat 2025.300 kb on + strandat 2025.301 kb on - strandat 2025.309 kb on + strandat 2025.309 kb on + strandat 2025.310 kb on - strandat 2025.432 kb on + strand, within RR42_RS09555at 2025.432 kb on + strand, within RR42_RS09555at 2025.432 kb on + strand, within RR42_RS09555

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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2,021,914 - RR42_RS09535 0.89 +0.8
2,022,170 - -1.4
2,022,185 + -0.1
2,022,185 + +0.3
2,022,287 + +0.4
2,022,338 + -0.8
2,022,339 - -0.2
2,022,339 - +0.8
2,022,339 - +0.2
2,022,435 + RR42_RS09540 0.27 +0.2
2,022,435 + RR42_RS09540 0.27 -0.2
2,022,436 - RR42_RS09540 0.27 +1.4
2,022,445 - RR42_RS09540 0.28 -0.6
2,022,445 - RR42_RS09540 0.28 +0.4
2,022,575 - RR42_RS09540 0.52 -0.8
2,022,674 + RR42_RS09540 0.69 +0.4
2,022,675 - RR42_RS09540 0.69 -0.2
2,022,882 + -0.2
2,022,883 - -0.2
2,022,883 - -0.4
2,022,969 + +0.3
2,022,969 + -0.4
2,022,970 - -0.4
2,022,970 - -0.1
2,022,970 - -0.4
2,022,973 + -0.2
2,023,024 - -0.2
2,023,233 + RR42_RS09545 0.22 +0.6
2,023,233 + RR42_RS09545 0.22 -0.2
2,023,234 - RR42_RS09545 0.22 +0.1
2,023,234 - RR42_RS09545 0.22 +0.0
2,023,305 - RR42_RS09545 0.27 +0.9
2,023,305 - RR42_RS09545 0.27 -0.4
2,023,473 + RR42_RS09545 0.37 -0.0
2,023,526 - RR42_RS09545 0.41 -0.3
2,023,812 + RR42_RS09545 0.59 -0.4
2,023,813 - RR42_RS09545 0.59 +0.3
2,023,813 - RR42_RS09545 0.59 -1.6
2,023,890 + RR42_RS09545 0.64 +1.2
2,023,891 - RR42_RS09545 0.64 +0.5
2,024,166 + RR42_RS09545 0.81 -1.6
2,024,167 - RR42_RS09545 0.81 -0.2
2,024,878 + RR42_RS09550 0.67 -0.5
2,024,878 + RR42_RS09550 0.67 +0.0
2,024,878 + RR42_RS09550 0.67 +1.0
2,024,878 + RR42_RS09550 0.67 -0.2
2,024,878 + RR42_RS09550 0.67 -1.1
2,024,878 + RR42_RS09550 0.67 -1.0
2,024,879 - RR42_RS09550 0.67 +0.5
2,024,900 + RR42_RS09550 0.71 +0.2
2,024,920 + RR42_RS09550 0.76 +0.3
2,024,920 + RR42_RS09550 0.76 +0.1
2,024,920 + RR42_RS09550 0.76 -0.3
2,024,931 + RR42_RS09550 0.78 -0.9
2,024,932 - RR42_RS09550 0.79 -0.2
2,024,932 - RR42_RS09550 0.79 +0.6
2,024,980 - RR42_RS09550 0.89 +0.1
2,024,980 - RR42_RS09550 0.89 +0.6
2,025,016 - -0.5
2,025,016 - -0.5
2,025,025 + +0.8
2,025,025 + +0.6
2,025,025 + +0.1
2,025,073 + +0.3
2,025,111 + +0.1
2,025,112 - -0.1
2,025,112 - -0.1
2,025,112 - -0.8
2,025,132 + +0.6
2,025,132 + +2.7
2,025,133 - +0.0
2,025,133 - +0.2
2,025,133 - +1.2
2,025,218 - -0.1
2,025,244 + -1.2
2,025,300 + +1.9
2,025,300 + -0.0
2,025,301 - +0.5
2,025,309 + -0.0
2,025,309 + +1.0
2,025,310 - +0.8
2,025,432 + RR42_RS09555 0.25 -1.4
2,025,432 + RR42_RS09555 0.25 +0.4
2,025,432 + RR42_RS09555 0.25 -0.4

Or see this region's nucleotide sequence