Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS05500

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS05490 and RR42_RS05495 are separated by 128 nucleotidesRR42_RS05495 and RR42_RS05500 are separated by 46 nucleotidesRR42_RS05500 and RR42_RS05505 are separated by 208 nucleotidesRR42_RS05505 and RR42_RS05510 are separated by 164 nucleotidesRR42_RS05510 and RR42_RS05515 are separated by 6 nucleotides RR42_RS05490: RR42_RS05490 - NAD(P)H-quinone oxidoreductase, at 1,212,370 to 1,213,374 _RS05490 RR42_RS05495: RR42_RS05495 - triosephosphate isomerase, at 1,213,503 to 1,214,240 _RS05495 RR42_RS05500: RR42_RS05500 - preprotein translocase subunit SecG, at 1,214,287 to 1,214,655 _RS05500 RR42_RS05505: RR42_RS05505 - tRNA-Leu, at 1,214,864 to 1,214,948 _RS05505 RR42_RS05510: RR42_RS05510 - NADH:ubiquinone oxidoreductase subunit A, at 1,215,113 to 1,215,472 _RS05510 RR42_RS05515: RR42_RS05515 - NADH-quinone oxidoreductase subunit B, at 1,215,479 to 1,215,961 _RS05515 Position (kb) 1214 1215Strain fitness (log2 ratio) -1 0 1at 1214.521 kb on + strand, within RR42_RS05500at 1214.674 kb on + strandat 1214.674 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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1,214,521 + RR42_RS05500 0.63 -1.0
1,214,674 + +1.1
1,214,674 + +0.2

Or see this region's nucleotide sequence