Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS05300 and RR42_RS05305 are separated by 282 nucleotides RR42_RS05305 and RR42_RS05310 are separated by 95 nucleotides RR42_RS05310 and RR42_RS05315 are separated by 218 nucleotides
RR42_RS05300: RR42_RS05300 - TonB-dependent receptor, at 1,170,036 to 1,172,360
_RS05300
RR42_RS05305: RR42_RS05305 - NADP oxidoreductase, at 1,172,643 to 1,173,416
_RS05305
RR42_RS05310: RR42_RS05310 - hypothetical protein, at 1,173,512 to 1,173,940
_RS05310
RR42_RS05315: RR42_RS05315 - fatty acid desaturase, at 1,174,159 to 1,175,259
_RS05315
Position (kb)
1172
1173
1174 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1171.767 kb on + strand, within RR42_RS05300 at 1171.846 kb on + strand, within RR42_RS05300 at 1171.846 kb on + strand, within RR42_RS05300 at 1171.846 kb on + strand, within RR42_RS05300 at 1171.847 kb on - strand, within RR42_RS05300 at 1171.958 kb on - strand, within RR42_RS05300 at 1172.011 kb on + strand, within RR42_RS05300 at 1172.011 kb on + strand, within RR42_RS05300 at 1172.043 kb on + strand, within RR42_RS05300 at 1172.044 kb on - strand, within RR42_RS05300 at 1172.458 kb on + strand at 1172.458 kb on + strand at 1172.458 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.462 kb on + strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.463 kb on - strand at 1172.467 kb on + strand at 1172.468 kb on - strand at 1172.967 kb on + strand, within RR42_RS05305 at 1172.967 kb on + strand, within RR42_RS05305 at 1172.968 kb on - strand, within RR42_RS05305 at 1172.968 kb on - strand, within RR42_RS05305 at 1172.997 kb on + strand, within RR42_RS05305 at 1173.115 kb on - strand, within RR42_RS05305 at 1173.588 kb on + strand, within RR42_RS05310 at 1173.589 kb on - strand, within RR42_RS05310 at 1173.732 kb on + strand, within RR42_RS05310 at 1173.822 kb on - strand, within RR42_RS05310 at 1174.029 kb on + strand at 1174.030 kb on - strand at 1174.086 kb on + strand at 1174.094 kb on + strand at 1174.095 kb on - strand at 1174.095 kb on - strand at 1174.232 kb on + strand at 1174.233 kb on - strand at 1174.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,171,767 + RR42_RS05300 0.74 -2.3 1,171,846 + RR42_RS05300 0.78 +1.7 1,171,846 + RR42_RS05300 0.78 -0.7 1,171,846 + RR42_RS05300 0.78 +0.2 1,171,847 - RR42_RS05300 0.78 +0.7 1,171,958 - RR42_RS05300 0.83 -0.3 1,172,011 + RR42_RS05300 0.85 -0.2 1,172,011 + RR42_RS05300 0.85 -2.9 1,172,043 + RR42_RS05300 0.86 -0.9 1,172,044 - RR42_RS05300 0.86 -0.1 1,172,458 + -0.9 1,172,458 + -0.7 1,172,458 + -0.3 1,172,462 + -0.8 1,172,462 + -0.7 1,172,462 + -0.3 1,172,462 + -0.1 1,172,463 - -1.0 1,172,463 - +0.4 1,172,463 - +0.1 1,172,463 - +0.9 1,172,463 - +0.4 1,172,467 + +0.2 1,172,468 - +0.1 1,172,967 + RR42_RS05305 0.42 -0.6 1,172,967 + RR42_RS05305 0.42 -0.3 1,172,968 - RR42_RS05305 0.42 -2.0 1,172,968 - RR42_RS05305 0.42 -1.5 1,172,997 + RR42_RS05305 0.46 +0.0 1,173,115 - RR42_RS05305 0.61 -2.3 1,173,588 + RR42_RS05310 0.18 -0.6 1,173,589 - RR42_RS05310 0.18 -1.4 1,173,732 + RR42_RS05310 0.51 -0.1 1,173,822 - RR42_RS05310 0.72 +1.0 1,174,029 + -0.7 1,174,030 - -0.1 1,174,086 + -1.7 1,174,094 + -1.0 1,174,095 - +1.0 1,174,095 - -0.5 1,174,232 + +0.1 1,174,233 - -0.3 1,174,233 - -1.5
Or see this region's nucleotide sequence