Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03625 and RR42_RS03630 are separated by 335 nucleotides RR42_RS03630 and RR42_RS03635 are separated by 97 nucleotides RR42_RS03635 and RR42_RS03640 are separated by 61 nucleotides RR42_RS03640 and RR42_RS03645 are separated by 192 nucleotides RR42_RS03645 and RR42_RS03650 are separated by 95 nucleotides
RR42_RS03625: RR42_RS03625 - hypothetical protein, at 808,311 to 808,550
_RS03625
RR42_RS03630: RR42_RS03630 - cold-shock protein, at 808,886 to 809,089
_RS03630
RR42_RS03635: RR42_RS03635 - trypsin-like serine protease, at 809,187 to 810,089
_RS03635
RR42_RS03640: RR42_RS03640 - hypothetical protein, at 810,151 to 810,435
_RS03640
RR42_RS03645: RR42_RS03645 - hypothetical protein, at 810,628 to 810,951
_RS03645
RR42_RS03650: RR42_RS03650 - hypothetical protein, at 811,047 to 811,331
_RS03650
Position (kb) 809
810
811 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 808.278 kb on - strand at 808.387 kb on + strand, within RR42_RS03625 at 808.388 kb on - strand, within RR42_RS03625 at 808.482 kb on + strand, within RR42_RS03625 at 808.482 kb on + strand, within RR42_RS03625 at 808.483 kb on - strand, within RR42_RS03625 at 808.483 kb on - strand, within RR42_RS03625 at 808.483 kb on - strand, within RR42_RS03625 at 808.582 kb on + strand at 808.583 kb on - strand at 808.599 kb on + strand at 808.599 kb on + strand at 808.601 kb on + strand at 808.620 kb on + strand at 808.620 kb on + strand at 808.620 kb on + strand at 808.620 kb on + strand at 808.621 kb on - strand at 808.621 kb on - strand at 808.621 kb on - strand at 808.621 kb on - strand at 808.716 kb on + strand at 808.730 kb on - strand at 808.865 kb on - strand at 808.871 kb on + strand at 808.872 kb on - strand at 808.872 kb on - strand at 809.033 kb on + strand, within RR42_RS03630 at 809.087 kb on + strand at 809.111 kb on - strand at 809.179 kb on - strand at 809.192 kb on + strand at 809.193 kb on - strand at 809.677 kb on - strand, within RR42_RS03635 at 809.890 kb on - strand, within RR42_RS03635 at 809.890 kb on - strand, within RR42_RS03635 at 810.087 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.117 kb on + strand at 810.118 kb on - strand at 810.175 kb on + strand at 810.176 kb on - strand at 810.192 kb on - strand, within RR42_RS03640 at 810.192 kb on - strand, within RR42_RS03640 at 810.193 kb on + strand, within RR42_RS03640 at 810.193 kb on + strand, within RR42_RS03640 at 810.194 kb on - strand, within RR42_RS03640 at 810.206 kb on - strand, within RR42_RS03640 at 810.206 kb on - strand, within RR42_RS03640 at 810.632 kb on + strand at 810.632 kb on + strand at 810.632 kb on + strand at 810.633 kb on - strand at 810.633 kb on - strand at 810.633 kb on - strand at 810.650 kb on + strand at 810.650 kb on + strand at 810.650 kb on + strand at 810.753 kb on + strand, within RR42_RS03645 at 810.753 kb on + strand, within RR42_RS03645 at 810.753 kb on + strand, within RR42_RS03645 at 810.833 kb on + strand, within RR42_RS03645 at 810.834 kb on - strand, within RR42_RS03645 at 810.834 kb on - strand, within RR42_RS03645 at 810.835 kb on + strand, within RR42_RS03645 at 810.835 kb on + strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.836 kb on - strand, within RR42_RS03645 at 810.842 kb on + strand, within RR42_RS03645 at 810.843 kb on - strand, within RR42_RS03645 at 810.950 kb on - strand at 811.055 kb on + strand at 811.056 kb on - strand at 811.071 kb on + strand at 811.072 kb on - strand at 811.072 kb on - strand at 811.072 kb on - strand at 811.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 808,278 - +0.2 808,387 + RR42_RS03625 0.32 +0.5 808,388 - RR42_RS03625 0.32 -0.1 808,482 + RR42_RS03625 0.71 +0.2 808,482 + RR42_RS03625 0.71 +0.3 808,483 - RR42_RS03625 0.72 +0.4 808,483 - RR42_RS03625 0.72 -0.6 808,483 - RR42_RS03625 0.72 -0.8 808,582 + -0.0 808,583 - -1.1 808,599 + +0.4 808,599 + +0.0 808,601 + +0.8 808,620 + -0.3 808,620 + +0.5 808,620 + -0.7 808,620 + +0.6 808,621 - +0.7 808,621 - -0.6 808,621 - -1.4 808,621 - +1.2 808,716 + -0.6 808,730 - +1.2 808,865 - +0.7 808,871 + +0.5 808,872 - +0.7 808,872 - +0.4 809,033 + RR42_RS03630 0.72 -0.2 809,087 + +0.2 809,111 - +0.5 809,179 - -0.8 809,192 + +0.5 809,193 - +0.2 809,677 - RR42_RS03635 0.54 +0.3 809,890 - RR42_RS03635 0.78 +0.5 809,890 - RR42_RS03635 0.78 +1.1 810,087 + +0.4 810,117 + -0.1 810,117 + -2.5 810,117 + -0.1 810,117 + -0.6 810,118 - -0.5 810,175 + +0.1 810,176 - -0.4 810,192 - RR42_RS03640 0.14 +0.3 810,192 - RR42_RS03640 0.14 +0.8 810,193 + RR42_RS03640 0.15 -0.8 810,193 + RR42_RS03640 0.15 +1.2 810,194 - RR42_RS03640 0.15 +0.2 810,206 - RR42_RS03640 0.19 +0.8 810,206 - RR42_RS03640 0.19 +0.9 810,632 + +0.6 810,632 + -0.3 810,632 + -0.3 810,633 - +0.4 810,633 - -0.3 810,633 - +0.6 810,650 + +0.8 810,650 + -0.7 810,650 + +0.6 810,753 + RR42_RS03645 0.39 -0.5 810,753 + RR42_RS03645 0.39 +0.3 810,753 + RR42_RS03645 0.39 -0.2 810,833 + RR42_RS03645 0.63 -0.2 810,834 - RR42_RS03645 0.64 +1.4 810,834 - RR42_RS03645 0.64 +1.8 810,835 + RR42_RS03645 0.64 +0.2 810,835 + RR42_RS03645 0.64 +0.1 810,836 - RR42_RS03645 0.64 +1.2 810,836 - RR42_RS03645 0.64 -0.8 810,836 - RR42_RS03645 0.64 +1.5 810,836 - RR42_RS03645 0.64 +0.8 810,842 + RR42_RS03645 0.66 -0.5 810,843 - RR42_RS03645 0.66 +0.4 810,950 - +0.3 811,055 + -0.1 811,056 - +3.8 811,071 + +1.1 811,072 - +0.2 811,072 - -0.3 811,072 - +0.4 811,072 - +0.1
Or see this region's nucleotide sequence