Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03390 and RR42_RS03395 are separated by 137 nucleotides RR42_RS03395 and RR42_RS03400 are separated by 179 nucleotides
RR42_RS03390: RR42_RS03390 - ABC transporter substrate-binding protein, at 754,848 to 755,816
_RS03390
RR42_RS03395: RR42_RS03395 - membrane protein, at 755,954 to 756,523
_RS03395
RR42_RS03400: RR42_RS03400 - permease, at 756,703 to 757,596
_RS03400
Position (kb) 755
756
757 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 755.189 kb on - strand, within RR42_RS03390 at 755.359 kb on + strand, within RR42_RS03390 at 755.365 kb on + strand, within RR42_RS03390 at 755.366 kb on - strand, within RR42_RS03390 at 755.407 kb on + strand, within RR42_RS03390 at 755.407 kb on + strand, within RR42_RS03390 at 755.408 kb on - strand, within RR42_RS03390 at 755.408 kb on - strand, within RR42_RS03390 at 755.497 kb on + strand, within RR42_RS03390 at 755.563 kb on + strand, within RR42_RS03390 at 755.865 kb on + strand at 755.865 kb on + strand at 756.244 kb on + strand, within RR42_RS03395 at 756.245 kb on - strand, within RR42_RS03395 at 756.245 kb on - strand, within RR42_RS03395 at 756.360 kb on + strand, within RR42_RS03395 at 756.360 kb on + strand, within RR42_RS03395 at 756.580 kb on + strand at 756.581 kb on - strand at 756.581 kb on - strand at 756.611 kb on + strand at 756.612 kb on - strand at 756.612 kb on - strand at 756.653 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.688 kb on - strand at 756.691 kb on + strand at 756.818 kb on - strand, within RR42_RS03400 at 756.940 kb on + strand, within RR42_RS03400 at 756.941 kb on - strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.018 kb on + strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.019 kb on - strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.483 kb on + strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.484 kb on - strand, within RR42_RS03400 at 757.485 kb on + strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400 at 757.486 kb on - strand, within RR42_RS03400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 755,189 - RR42_RS03390 0.35 -0.5 755,359 + RR42_RS03390 0.53 +0.3 755,365 + RR42_RS03390 0.53 -2.9 755,366 - RR42_RS03390 0.53 -0.0 755,407 + RR42_RS03390 0.58 +0.9 755,407 + RR42_RS03390 0.58 -1.6 755,408 - RR42_RS03390 0.58 -0.8 755,408 - RR42_RS03390 0.58 -0.1 755,497 + RR42_RS03390 0.67 +1.4 755,563 + RR42_RS03390 0.74 -0.8 755,865 + -0.0 755,865 + +0.6 756,244 + RR42_RS03395 0.51 -0.3 756,245 - RR42_RS03395 0.51 -0.3 756,245 - RR42_RS03395 0.51 -1.8 756,360 + RR42_RS03395 0.71 -0.5 756,360 + RR42_RS03395 0.71 -1.5 756,580 + +0.3 756,581 - +0.3 756,581 - -1.4 756,611 + -0.0 756,612 - -0.1 756,612 - +0.1 756,653 - -0.9 756,688 - -0.5 756,688 - +1.0 756,688 - +0.9 756,691 + -1.2 756,818 - RR42_RS03400 0.13 +0.7 756,940 + RR42_RS03400 0.27 -0.5 756,941 - RR42_RS03400 0.27 +0.1 757,018 + RR42_RS03400 0.35 -0.6 757,018 + RR42_RS03400 0.35 +0.1 757,019 - RR42_RS03400 0.35 +0.1 757,019 - RR42_RS03400 0.35 +1.0 757,019 - RR42_RS03400 0.35 -0.9 757,483 + RR42_RS03400 0.87 +0.4 757,483 + RR42_RS03400 0.87 -0.5 757,484 - RR42_RS03400 0.87 +0.8 757,484 - RR42_RS03400 0.87 -0.8 757,484 - RR42_RS03400 0.87 +0.3 757,484 - RR42_RS03400 0.87 +0.4 757,485 + RR42_RS03400 0.87 +0.2 757,486 - RR42_RS03400 0.88 +0.7 757,486 - RR42_RS03400 0.88 +0.5
Or see this region's nucleotide sequence