Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03015 and RR42_RS03020 are separated by 354 nucleotides RR42_RS03020 and RR42_RS03025 are separated by 4 nucleotides
RR42_RS03015: RR42_RS03015 - enoyl-CoA hydratase, at 667,753 to 668,550
_RS03015
RR42_RS03020: RR42_RS03020 - MarR family transcriptional regulator, at 668,905 to 669,366
_RS03020
RR42_RS03025: RR42_RS03025 - Inner membrane protein (FUSC-like) required for 4-hydroxybenzoate transport, together with NodT, MFP, and DUF1656 proteins (RR42_RS03040, RR42_RS03035, and RR42_RS03030) <small>(from data)</small>, at 669,371 to 671,365
_RS03025
Position (kb) 668
669
670 Strain fitness (log2 ratio) -2
-1
0
1
2 at 667.969 kb on + strand, within RR42_RS03015 at 667.969 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.972 kb on + strand, within RR42_RS03015 at 667.973 kb on - strand, within RR42_RS03015 at 668.198 kb on - strand, within RR42_RS03015 at 668.729 kb on - strand at 668.730 kb on + strand at 668.731 kb on - strand at 668.838 kb on - strand at 669.085 kb on + strand, within RR42_RS03020 at 669.086 kb on - strand, within RR42_RS03020 at 669.364 kb on + strand at 669.364 kb on + strand at 669.455 kb on + strand at 669.497 kb on + strand at 669.497 kb on + strand at 669.498 kb on - strand at 669.498 kb on - strand at 669.498 kb on - strand at 669.500 kb on - strand at 669.500 kb on - strand at 669.500 kb on - strand at 669.695 kb on + strand, within RR42_RS03025 at 669.695 kb on + strand, within RR42_RS03025 at 669.695 kb on + strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.696 kb on - strand, within RR42_RS03025 at 669.716 kb on + strand, within RR42_RS03025 at 669.717 kb on - strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.718 kb on + strand, within RR42_RS03025 at 669.719 kb on - strand, within RR42_RS03025 at 669.739 kb on - strand, within RR42_RS03025 at 669.918 kb on - strand, within RR42_RS03025 at 670.023 kb on - strand, within RR42_RS03025 at 670.111 kb on + strand, within RR42_RS03025
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 667,969 + RR42_RS03015 0.27 +0.1 667,969 + RR42_RS03015 0.27 +0.1 667,972 + RR42_RS03015 0.27 +2.0 667,972 + RR42_RS03015 0.27 -0.5 667,972 + RR42_RS03015 0.27 -0.9 667,973 - RR42_RS03015 0.28 +0.8 668,198 - RR42_RS03015 0.56 -0.8 668,729 - +1.4 668,730 + +0.3 668,731 - +0.9 668,838 - -1.2 669,085 + RR42_RS03020 0.39 -0.9 669,086 - RR42_RS03020 0.39 -1.0 669,364 + +1.3 669,364 + +0.1 669,455 + +0.7 669,497 + -1.2 669,497 + -0.2 669,498 - +0.2 669,498 - -0.0 669,498 - -2.7 669,500 - +0.2 669,500 - -0.2 669,500 - +0.1 669,695 + RR42_RS03025 0.16 -0.6 669,695 + RR42_RS03025 0.16 +0.0 669,695 + RR42_RS03025 0.16 -1.6 669,696 - RR42_RS03025 0.16 +0.7 669,696 - RR42_RS03025 0.16 +0.2 669,696 - RR42_RS03025 0.16 +0.7 669,696 - RR42_RS03025 0.16 -0.1 669,696 - RR42_RS03025 0.16 +0.7 669,716 + RR42_RS03025 0.17 -0.1 669,717 - RR42_RS03025 0.17 -0.2 669,718 + RR42_RS03025 0.17 +0.8 669,718 + RR42_RS03025 0.17 +2.0 669,718 + RR42_RS03025 0.17 +0.4 669,718 + RR42_RS03025 0.17 -0.5 669,719 - RR42_RS03025 0.17 -0.5 669,739 - RR42_RS03025 0.18 +0.1 669,918 - RR42_RS03025 0.27 +0.4 670,023 - RR42_RS03025 0.33 +0.9 670,111 + RR42_RS03025 0.37 +0.3
Or see this region's nucleotide sequence