Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03000 and RR42_RS03005 are separated by 162 nucleotides RR42_RS03005 and RR42_RS03010 are separated by 44 nucleotides
RR42_RS03000: RR42_RS03000 - ABC transporter substrate-binding protein, at 664,589 to 665,557
_RS03000
RR42_RS03005: RR42_RS03005 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 665,720 to 666,484
_RS03005
RR42_RS03010: RR42_RS03010 - acetyl-CoA acetyltransferase, at 666,529 to 667,707
_RS03010
Position (kb) 665
666
667 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 664.799 kb on + strand, within RR42_RS03000 at 665.097 kb on + strand, within RR42_RS03000 at 665.097 kb on + strand, within RR42_RS03000 at 665.098 kb on - strand, within RR42_RS03000 at 665.232 kb on + strand, within RR42_RS03000 at 665.247 kb on + strand, within RR42_RS03000 at 665.256 kb on + strand, within RR42_RS03000 at 665.256 kb on + strand, within RR42_RS03000 at 665.256 kb on + strand, within RR42_RS03000 at 665.257 kb on - strand, within RR42_RS03000 at 665.257 kb on - strand, within RR42_RS03000 at 665.298 kb on + strand, within RR42_RS03000 at 665.299 kb on - strand, within RR42_RS03000 at 665.539 kb on + strand at 665.540 kb on - strand at 665.791 kb on + strand at 665.792 kb on - strand at 665.902 kb on + strand, within RR42_RS03005 at 665.903 kb on - strand, within RR42_RS03005 at 666.006 kb on + strand, within RR42_RS03005 at 666.033 kb on + strand, within RR42_RS03005 at 666.033 kb on + strand, within RR42_RS03005 at 666.489 kb on + strand at 666.770 kb on + strand, within RR42_RS03010 at 667.379 kb on + strand, within RR42_RS03010 at 667.379 kb on + strand, within RR42_RS03010
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 664,799 + RR42_RS03000 0.22 -0.5 665,097 + RR42_RS03000 0.52 +0.9 665,097 + RR42_RS03000 0.52 -2.1 665,098 - RR42_RS03000 0.53 +0.2 665,232 + RR42_RS03000 0.66 +0.5 665,247 + RR42_RS03000 0.68 +0.3 665,256 + RR42_RS03000 0.69 +0.5 665,256 + RR42_RS03000 0.69 +1.0 665,256 + RR42_RS03000 0.69 +2.9 665,257 - RR42_RS03000 0.69 +0.2 665,257 - RR42_RS03000 0.69 -1.1 665,298 + RR42_RS03000 0.73 +0.1 665,299 - RR42_RS03000 0.73 -0.2 665,539 + +0.5 665,540 - +0.2 665,791 + +0.7 665,792 - +0.0 665,902 + RR42_RS03005 0.24 -1.5 665,903 - RR42_RS03005 0.24 -1.2 666,006 + RR42_RS03005 0.37 +0.3 666,033 + RR42_RS03005 0.41 +3.2 666,033 + RR42_RS03005 0.41 -1.9 666,489 + +0.2 666,770 + RR42_RS03010 0.20 +0.2 667,379 + RR42_RS03010 0.72 +0.2 667,379 + RR42_RS03010 0.72 -1.1
Or see this region's nucleotide sequence