Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02845 and RR42_RS02850 are separated by 11 nucleotides RR42_RS02850 and RR42_RS02855 are separated by 40 nucleotides
RR42_RS02845: RR42_RS02845 - 16S rRNA maturation RNase YbeY, at 634,388 to 635,143
_RS02845
RR42_RS02850: RR42_RS02850 - phoH-like protein, at 635,155 to 636,150
_RS02850
RR42_RS02855: RR42_RS02855 - dimethylallyladenosine tRNA methylthiotransferase, at 636,191 to 637,543
_RS02855
Position (kb) 635
636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 634.342 kb on + strand at 634.342 kb on + strand at 634.343 kb on - strand at 634.353 kb on - strand at 634.383 kb on + strand at 634.383 kb on + strand at 634.383 kb on + strand at 634.383 kb on + strand at 634.384 kb on - strand at 634.384 kb on - strand at 634.389 kb on + strand at 634.389 kb on + strand at 634.390 kb on - strand at 634.390 kb on - strand at 634.422 kb on + strand at 634.423 kb on - strand at 634.423 kb on - strand at 634.423 kb on - strand at 634.423 kb on - strand at 634.423 kb on - strand at 635.228 kb on - strand at 635.466 kb on - strand, within RR42_RS02850 at 635.466 kb on - strand, within RR42_RS02850 at 635.550 kb on - strand, within RR42_RS02850 at 635.564 kb on + strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.565 kb on - strand, within RR42_RS02850 at 635.699 kb on + strand, within RR42_RS02850 at 635.700 kb on - strand, within RR42_RS02850 at 635.874 kb on - strand, within RR42_RS02850 at 635.958 kb on - strand, within RR42_RS02850 at 636.272 kb on - strand at 636.272 kb on - strand at 636.536 kb on - strand, within RR42_RS02855 at 636.688 kb on - strand, within RR42_RS02855 at 636.789 kb on + strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.790 kb on - strand, within RR42_RS02855 at 636.838 kb on - strand, within RR42_RS02855 at 636.931 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.005 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.060 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855 at 637.069 kb on - strand, within RR42_RS02855
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 634,342 + -0.1 634,342 + +0.4 634,343 - +0.1 634,353 - +0.6 634,383 + -1.3 634,383 + -0.6 634,383 + +0.5 634,383 + -0.7 634,384 - +0.1 634,384 - +0.5 634,389 + -0.0 634,389 + +0.1 634,390 - +0.2 634,390 - -1.9 634,422 + -0.4 634,423 - +0.0 634,423 - +0.6 634,423 - -0.8 634,423 - -0.9 634,423 - -1.2 635,228 - +0.3 635,466 - RR42_RS02850 0.31 -3.2 635,466 - RR42_RS02850 0.31 -0.1 635,550 - RR42_RS02850 0.40 -1.9 635,564 + RR42_RS02850 0.41 +0.9 635,565 - RR42_RS02850 0.41 -1.6 635,565 - RR42_RS02850 0.41 +0.7 635,699 + RR42_RS02850 0.55 -1.5 635,700 - RR42_RS02850 0.55 -0.0 635,874 - RR42_RS02850 0.72 -2.1 635,958 - RR42_RS02850 0.81 -0.8 636,272 - -0.3 636,272 - +0.1 636,536 - RR42_RS02855 0.25 +0.6 636,688 - RR42_RS02855 0.37 -0.1 636,789 + RR42_RS02855 0.44 -1.0 636,790 - RR42_RS02855 0.44 +1.1 636,790 - RR42_RS02855 0.44 +0.2 636,790 - RR42_RS02855 0.44 -0.0 636,838 - RR42_RS02855 0.48 -0.1 636,931 - RR42_RS02855 0.55 -0.2 637,005 - RR42_RS02855 0.60 +0.2 637,005 - RR42_RS02855 0.60 -0.4 637,060 - RR42_RS02855 0.64 -0.2 637,060 - RR42_RS02855 0.64 -0.8 637,069 - RR42_RS02855 0.65 -0.9 637,069 - RR42_RS02855 0.65 -2.3
Or see this region's nucleotide sequence