Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02320 and RR42_RS02325 are separated by 12 nucleotides RR42_RS02325 and RR42_RS02330 are separated by 34 nucleotides
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
RR42_RS02325: RR42_RS02325 - glucoamylase, at 520,917 to 522,755
_RS02325
RR42_RS02330: RR42_RS02330 - trehalose-6-phosphate synthase, at 522,790 to 524,190
_RS02330
Position (kb) 520
521
522
523 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320 at 521.169 kb on + strand, within RR42_RS02325 at 521.169 kb on + strand, within RR42_RS02325 at 521.170 kb on - strand, within RR42_RS02325 at 521.235 kb on - strand, within RR42_RS02325 at 521.271 kb on + strand, within RR42_RS02325 at 521.272 kb on - strand, within RR42_RS02325 at 521.406 kb on + strand, within RR42_RS02325 at 521.407 kb on - strand, within RR42_RS02325 at 521.408 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.458 kb on - strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.581 kb on - strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.829 kb on + strand, within RR42_RS02325 at 521.889 kb on + strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.085 kb on - strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.264 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.700 kb on + strand at 522.700 kb on + strand at 522.701 kb on - strand at 522.790 kb on - strand at 522.800 kb on + strand at 522.801 kb on - strand at 523.030 kb on + strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.042 kb on + strand, within RR42_RS02330 at 523.075 kb on + strand, within RR42_RS02330 at 523.121 kb on - strand, within RR42_RS02330 at 523.198 kb on + strand, within RR42_RS02330 at 523.269 kb on + strand, within RR42_RS02330 at 523.342 kb on + strand, within RR42_RS02330 at 523.433 kb on - strand, within RR42_RS02330 at 523.540 kb on + strand, within RR42_RS02330 at 523.541 kb on - strand, within RR42_RS02330 at 523.561 kb on + strand, within RR42_RS02330 at 523.562 kb on - strand, within RR42_RS02330 at 523.603 kb on + strand, within RR42_RS02330 at 523.726 kb on + strand, within RR42_RS02330 at 523.727 kb on - strand, within RR42_RS02330
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 519,918 - -0.4 519,918 - +0.4 520,066 + +0.2 520,112 + -0.2 520,113 - +0.6 520,130 - -2.0 520,497 + RR42_RS02320 0.48 -1.2 520,646 + RR42_RS02320 0.67 -0.6 520,697 + RR42_RS02320 0.74 -3.2 520,697 + RR42_RS02320 0.74 -1.7 520,698 - RR42_RS02320 0.74 -0.7 521,169 + RR42_RS02325 0.14 +0.0 521,169 + RR42_RS02325 0.14 -0.3 521,170 - RR42_RS02325 0.14 +0.8 521,235 - RR42_RS02325 0.17 -0.9 521,271 + RR42_RS02325 0.19 -0.1 521,272 - RR42_RS02325 0.19 -1.5 521,406 + RR42_RS02325 0.27 +0.1 521,407 - RR42_RS02325 0.27 +0.8 521,408 + RR42_RS02325 0.27 -0.0 521,457 + RR42_RS02325 0.29 -1.0 521,457 + RR42_RS02325 0.29 +1.2 521,457 + RR42_RS02325 0.29 +0.6 521,457 + RR42_RS02325 0.29 -0.6 521,458 - RR42_RS02325 0.29 +0.1 521,580 + RR42_RS02325 0.36 +0.3 521,580 + RR42_RS02325 0.36 -0.5 521,580 + RR42_RS02325 0.36 -0.1 521,581 - RR42_RS02325 0.36 +0.0 521,651 + RR42_RS02325 0.40 +0.3 521,651 + RR42_RS02325 0.40 -0.1 521,671 - RR42_RS02325 0.41 -0.2 521,671 - RR42_RS02325 0.41 -0.6 521,829 + RR42_RS02325 0.50 +0.4 521,889 + RR42_RS02325 0.53 +2.6 522,010 - RR42_RS02325 0.59 -0.3 522,010 - RR42_RS02325 0.59 -0.6 522,019 + RR42_RS02325 0.60 -0.3 522,019 + RR42_RS02325 0.60 +0.6 522,019 + RR42_RS02325 0.60 -0.5 522,019 + RR42_RS02325 0.60 +1.2 522,085 - RR42_RS02325 0.64 +0.1 522,179 + RR42_RS02325 0.69 -1.1 522,179 + RR42_RS02325 0.69 +0.6 522,179 + RR42_RS02325 0.69 +0.7 522,264 + RR42_RS02325 0.73 -0.6 522,471 + RR42_RS02325 0.85 -0.1 522,471 + RR42_RS02325 0.85 -0.8 522,471 + RR42_RS02325 0.85 +0.5 522,472 - RR42_RS02325 0.85 +1.4 522,472 - RR42_RS02325 0.85 -0.2 522,472 - RR42_RS02325 0.85 +0.1 522,472 - RR42_RS02325 0.85 +0.5 522,473 + RR42_RS02325 0.85 -0.8 522,473 + RR42_RS02325 0.85 -0.3 522,474 - RR42_RS02325 0.85 -0.1 522,474 - RR42_RS02325 0.85 +0.3 522,474 - RR42_RS02325 0.85 +0.2 522,700 + +1.3 522,700 + +0.1 522,701 - +0.4 522,790 - +0.1 522,800 + +0.6 522,801 - +0.5 523,030 + RR42_RS02330 0.17 +0.7 523,034 - RR42_RS02330 0.17 -0.3 523,034 - RR42_RS02330 0.17 +0.5 523,042 + RR42_RS02330 0.18 +0.4 523,075 + RR42_RS02330 0.20 +0.1 523,121 - RR42_RS02330 0.24 +0.4 523,198 + RR42_RS02330 0.29 -2.0 523,269 + RR42_RS02330 0.34 -0.2 523,342 + RR42_RS02330 0.39 -0.5 523,433 - RR42_RS02330 0.46 +0.0 523,540 + RR42_RS02330 0.54 -0.2 523,541 - RR42_RS02330 0.54 -0.7 523,561 + RR42_RS02330 0.55 +0.6 523,562 - RR42_RS02330 0.55 -0.8 523,603 + RR42_RS02330 0.58 +0.5 523,726 + RR42_RS02330 0.67 -0.6 523,727 - RR42_RS02330 0.67 +0.5
Or see this region's nucleotide sequence