Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01650 and RR42_RS01655 are separated by 160 nucleotides RR42_RS01655 and RR42_RS01660 overlap by 10 nucleotides RR42_RS01660 and RR42_RS01665 are separated by 77 nucleotides
RR42_RS01650: RR42_RS01650 - branched-chain amino acid ABC transporter ATP-binding protein, at 376,432 to 377,247
_RS01650
RR42_RS01655: RR42_RS01655 - AMP-dependent synthetase, at 377,408 to 378,658
_RS01655
RR42_RS01660: RR42_RS01660 - hypothetical protein, at 378,649 to 379,047
_RS01660
RR42_RS01665: RR42_RS01665 - 2-nitropropane dioxygenase, at 379,125 to 380,102
_RS01665
Position (kb) 377
378
379 Strain fitness (log2 ratio) -1
0
1
2
3 at 376.456 kb on - strand at 376.468 kb on + strand at 376.468 kb on + strand at 376.469 kb on - strand at 376.469 kb on - strand at 376.760 kb on - strand, within RR42_RS01650 at 376.760 kb on - strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.811 kb on - strand, within RR42_RS01650 at 377.060 kb on - strand, within RR42_RS01650 at 377.074 kb on + strand, within RR42_RS01650 at 377.075 kb on - strand, within RR42_RS01650 at 377.152 kb on + strand, within RR42_RS01650 at 377.152 kb on + strand, within RR42_RS01650 at 377.153 kb on - strand, within RR42_RS01650 at 377.218 kb on + strand at 377.218 kb on + strand at 377.218 kb on + strand at 377.370 kb on - strand at 377.392 kb on - strand at 377.400 kb on + strand at 377.401 kb on - strand at 377.417 kb on + strand at 377.517 kb on - strand at 377.786 kb on + strand, within RR42_RS01655 at 377.786 kb on + strand, within RR42_RS01655 at 377.801 kb on + strand, within RR42_RS01655 at 378.089 kb on + strand, within RR42_RS01655 at 378.089 kb on + strand, within RR42_RS01655 at 378.090 kb on - strand, within RR42_RS01655 at 379.107 kb on + strand at 379.162 kb on + strand at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.358 kb on - strand, within RR42_RS01665 at 379.358 kb on - strand, within RR42_RS01665 at 379.390 kb on + strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 376,456 - -0.2 376,468 + -0.1 376,468 + -0.8 376,469 - -0.2 376,469 - -0.1 376,760 - RR42_RS01650 0.40 +3.0 376,760 - RR42_RS01650 0.40 +0.9 376,761 + RR42_RS01650 0.40 +0.1 376,761 + RR42_RS01650 0.40 -0.2 376,761 + RR42_RS01650 0.40 +1.0 376,811 - RR42_RS01650 0.46 -0.1 377,060 - RR42_RS01650 0.77 +0.1 377,074 + RR42_RS01650 0.79 +1.2 377,075 - RR42_RS01650 0.79 +0.0 377,152 + RR42_RS01650 0.88 +2.2 377,152 + RR42_RS01650 0.88 +0.6 377,153 - RR42_RS01650 0.88 +0.0 377,218 + -0.4 377,218 + -1.7 377,218 + +0.5 377,370 - -0.0 377,392 - +0.9 377,400 + +0.3 377,401 - -0.5 377,417 + +0.9 377,517 - -0.4 377,786 + RR42_RS01655 0.30 -1.0 377,786 + RR42_RS01655 0.30 +0.1 377,801 + RR42_RS01655 0.31 -0.4 378,089 + RR42_RS01655 0.54 -0.1 378,089 + RR42_RS01655 0.54 -1.6 378,090 - RR42_RS01655 0.55 -0.7 379,107 + -0.1 379,162 + +0.4 379,357 + RR42_RS01665 0.24 +0.3 379,357 + RR42_RS01665 0.24 -0.1 379,357 + RR42_RS01665 0.24 -0.7 379,357 + RR42_RS01665 0.24 +0.2 379,357 + RR42_RS01665 0.24 -0.9 379,358 - RR42_RS01665 0.24 -1.5 379,358 - RR42_RS01665 0.24 +0.5 379,390 + RR42_RS01665 0.27 -0.3 379,391 - RR42_RS01665 0.27 -0.2 379,391 - RR42_RS01665 0.27 +0.4 379,391 - RR42_RS01665 0.27 -0.2 379,391 - RR42_RS01665 0.27 -0.7 379,391 - RR42_RS01665 0.27 -1.1
Or see this region's nucleotide sequence