Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01025 and RR42_RS01030 are separated by 253 nucleotides RR42_RS01030 and RR42_RS01035 overlap by 2 nucleotides RR42_RS01035 and RR42_RS01040 are separated by 282 nucleotides
RR42_RS01025: RR42_RS01025 - GTPase, at 242,771 to 244,066
_RS01025
RR42_RS01030: RR42_RS01030 - molecular chaperone DnaK, at 244,320 to 244,739
_RS01030
RR42_RS01035: RR42_RS01035 - hypothetical protein, at 244,738 to 245,046
_RS01035
RR42_RS01040: RR42_RS01040 - cobalamin biosynthesis protein CobW, at 245,329 to 246,417
_RS01040
Position (kb) 244
245
246 Strain fitness (log2 ratio) -2
-1
0
1 at 243.786 kb on + strand, within RR42_RS01025 at 243.787 kb on - strand, within RR42_RS01025 at 244.041 kb on - strand at 244.122 kb on + strand at 244.189 kb on + strand at 244.190 kb on - strand at 244.190 kb on - strand at 244.195 kb on + strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 245.187 kb on - strand at 245.187 kb on - strand at 245.212 kb on - strand at 245.212 kb on - strand at 245.212 kb on - strand at 245.216 kb on + strand at 245.216 kb on + strand at 245.216 kb on + strand at 245.217 kb on - strand at 245.217 kb on - strand at 245.240 kb on - strand at 245.240 kb on - strand at 245.270 kb on - strand at 245.270 kb on - strand at 245.496 kb on + strand, within RR42_RS01040 at 245.496 kb on + strand, within RR42_RS01040 at 245.496 kb on + strand, within RR42_RS01040 at 245.497 kb on - strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.498 kb on + strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.499 kb on - strand, within RR42_RS01040 at 245.503 kb on + strand, within RR42_RS01040 at 245.503 kb on + strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.504 kb on - strand, within RR42_RS01040 at 245.513 kb on + strand, within RR42_RS01040 at 245.534 kb on + strand, within RR42_RS01040 at 245.534 kb on + strand, within RR42_RS01040
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 243,786 + RR42_RS01025 0.78 +1.1 243,787 - RR42_RS01025 0.78 +0.0 244,041 - +0.7 244,122 + -0.3 244,189 + +0.1 244,190 - +0.4 244,190 - -0.7 244,195 + -0.3 244,196 - +0.4 244,196 - -0.2 244,196 - -0.8 244,196 - +0.1 244,196 - +0.1 245,187 - -0.7 245,187 - -0.5 245,212 - -0.3 245,212 - +0.1 245,212 - +0.2 245,216 + -2.0 245,216 + -1.0 245,216 + -1.4 245,217 - +0.1 245,217 - +0.7 245,240 - -0.4 245,240 - +0.5 245,270 - -0.7 245,270 - +0.4 245,496 + RR42_RS01040 0.15 +0.5 245,496 + RR42_RS01040 0.15 +0.3 245,496 + RR42_RS01040 0.15 -0.2 245,497 - RR42_RS01040 0.15 -1.8 245,498 + RR42_RS01040 0.16 +0.1 245,498 + RR42_RS01040 0.16 -0.4 245,498 + RR42_RS01040 0.16 +1.6 245,498 + RR42_RS01040 0.16 -0.5 245,498 + RR42_RS01040 0.16 +0.6 245,498 + RR42_RS01040 0.16 -0.2 245,498 + RR42_RS01040 0.16 -0.9 245,498 + RR42_RS01040 0.16 +0.7 245,498 + RR42_RS01040 0.16 +0.9 245,498 + RR42_RS01040 0.16 +0.8 245,498 + RR42_RS01040 0.16 -0.7 245,498 + RR42_RS01040 0.16 +0.3 245,498 + RR42_RS01040 0.16 -1.7 245,498 + RR42_RS01040 0.16 -1.5 245,498 + RR42_RS01040 0.16 +0.4 245,499 - RR42_RS01040 0.16 -0.7 245,499 - RR42_RS01040 0.16 -0.4 245,499 - RR42_RS01040 0.16 +0.4 245,499 - RR42_RS01040 0.16 +0.3 245,503 + RR42_RS01040 0.16 +0.3 245,503 + RR42_RS01040 0.16 +0.9 245,504 - RR42_RS01040 0.16 +0.9 245,504 - RR42_RS01040 0.16 -1.1 245,504 - RR42_RS01040 0.16 -0.9 245,504 - RR42_RS01040 0.16 +0.4 245,504 - RR42_RS01040 0.16 +0.8 245,513 + RR42_RS01040 0.17 +1.1 245,534 + RR42_RS01040 0.19 -0.2 245,534 + RR42_RS01040 0.19 +0.9
Or see this region's nucleotide sequence