Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00860 and RR42_RS00865 are separated by 85 nucleotides RR42_RS00865 and RR42_RS00870 are separated by 72 nucleotides RR42_RS00870 and RR42_RS00875 are separated by 161 nucleotides
RR42_RS00860: RR42_RS00860 - MerR family transcriptional regulator, at 208,798 to 209,232
_RS00860
RR42_RS00865: RR42_RS00865 - phenylacetic acid degradation protein PaaI, at 209,318 to 209,776
_RS00865
RR42_RS00870: RR42_RS00870 - isovaleryl-CoA dehydrogenase, at 209,849 to 211,039
_RS00870
RR42_RS00875: RR42_RS00875 - isocitrate dehydrogenase, at 211,201 to 213,045
_RS00875
Position (kb)
209
210
211
212 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 208.882 kb on + strand, within RR42_RS00860 at 208.945 kb on - strand, within RR42_RS00860 at 209.036 kb on - strand, within RR42_RS00860 at 209.036 kb on - strand, within RR42_RS00860 at 209.151 kb on - strand, within RR42_RS00860 at 209.233 kb on + strand at 209.242 kb on - strand at 209.280 kb on - strand at 209.290 kb on - strand at 209.295 kb on - strand at 209.455 kb on + strand, within RR42_RS00865 at 209.455 kb on + strand, within RR42_RS00865 at 209.456 kb on - strand, within RR42_RS00865 at 209.456 kb on - strand, within RR42_RS00865 at 209.456 kb on - strand, within RR42_RS00865 at 209.456 kb on - strand, within RR42_RS00865 at 209.591 kb on + strand, within RR42_RS00865 at 209.591 kb on + strand, within RR42_RS00865 at 209.592 kb on - strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.691 kb on + strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.692 kb on - strand, within RR42_RS00865 at 209.693 kb on + strand, within RR42_RS00865 at 209.693 kb on + strand, within RR42_RS00865 at 209.693 kb on + strand, within RR42_RS00865 at 209.693 kb on + strand, within RR42_RS00865 at 209.694 kb on - strand, within RR42_RS00865 at 209.694 kb on - strand, within RR42_RS00865 at 209.694 kb on - strand, within RR42_RS00865 at 209.695 kb on + strand, within RR42_RS00865 at 209.695 kb on + strand, within RR42_RS00865 at 209.695 kb on + strand, within RR42_RS00865 at 209.695 kb on + strand, within RR42_RS00865 at 209.696 kb on - strand, within RR42_RS00865 at 209.696 kb on - strand, within RR42_RS00865 at 209.696 kb on - strand, within RR42_RS00865 at 209.764 kb on - strand at 209.801 kb on + strand at 209.802 kb on - strand at 209.802 kb on - strand at 209.802 kb on - strand at 209.901 kb on + strand at 209.901 kb on + strand at 209.901 kb on + strand at 209.901 kb on + strand at 209.901 kb on + strand at 209.902 kb on - strand at 209.902 kb on - strand at 209.902 kb on - strand at 209.902 kb on - strand at 209.902 kb on - strand at 209.902 kb on - strand at 209.902 kb on - strand at 211.155 kb on + strand at 211.184 kb on - strand at 211.240 kb on + strand at 211.381 kb on + strand at 211.381 kb on + strand at 211.426 kb on + strand, within RR42_RS00875 at 211.426 kb on + strand, within RR42_RS00875 at 211.427 kb on - strand, within RR42_RS00875 at 211.427 kb on - strand, within RR42_RS00875 at 211.441 kb on - strand, within RR42_RS00875 at 211.477 kb on + strand, within RR42_RS00875 at 211.478 kb on - strand, within RR42_RS00875 at 211.478 kb on - strand, within RR42_RS00875 at 211.564 kb on + strand, within RR42_RS00875 at 211.564 kb on + strand, within RR42_RS00875 at 211.565 kb on - strand, within RR42_RS00875 at 211.565 kb on - strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.612 kb on + strand, within RR42_RS00875 at 211.613 kb on - strand, within RR42_RS00875 at 211.658 kb on - strand, within RR42_RS00875 at 211.661 kb on - strand, within RR42_RS00875 at 211.711 kb on - strand, within RR42_RS00875 at 211.752 kb on + strand, within RR42_RS00875 at 211.759 kb on + strand, within RR42_RS00875 at 211.760 kb on - strand, within RR42_RS00875 at 211.760 kb on - strand, within RR42_RS00875 at 212.038 kb on + strand, within RR42_RS00875 at 212.038 kb on + strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875 at 212.039 kb on - strand, within RR42_RS00875
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 208,882 + RR42_RS00860 0.19 -0.5 208,945 - RR42_RS00860 0.34 -0.9 209,036 - RR42_RS00860 0.55 +0.2 209,036 - RR42_RS00860 0.55 -1.6 209,151 - RR42_RS00860 0.81 +0.4 209,233 + -0.1 209,242 - -0.3 209,280 - +0.3 209,290 - -1.6 209,295 - -0.7 209,455 + RR42_RS00865 0.30 +0.7 209,455 + RR42_RS00865 0.30 -0.1 209,456 - RR42_RS00865 0.30 -1.1 209,456 - RR42_RS00865 0.30 +1.4 209,456 - RR42_RS00865 0.30 -0.6 209,456 - RR42_RS00865 0.30 +0.4 209,591 + RR42_RS00865 0.59 -0.8 209,591 + RR42_RS00865 0.59 +0.4 209,592 - RR42_RS00865 0.60 -0.3 209,691 + RR42_RS00865 0.81 +0.0 209,691 + RR42_RS00865 0.81 -0.2 209,691 + RR42_RS00865 0.81 -0.5 209,691 + RR42_RS00865 0.81 -0.6 209,691 + RR42_RS00865 0.81 -0.3 209,691 + RR42_RS00865 0.81 +0.3 209,691 + RR42_RS00865 0.81 +0.1 209,691 + RR42_RS00865 0.81 -0.3 209,692 - RR42_RS00865 0.81 -0.9 209,692 - RR42_RS00865 0.81 -0.6 209,692 - RR42_RS00865 0.81 -0.0 209,692 - RR42_RS00865 0.81 -0.3 209,692 - RR42_RS00865 0.81 +0.2 209,692 - RR42_RS00865 0.81 -0.7 209,692 - RR42_RS00865 0.81 -0.7 209,692 - RR42_RS00865 0.81 -0.3 209,692 - RR42_RS00865 0.81 -0.0 209,692 - RR42_RS00865 0.81 +0.8 209,692 - RR42_RS00865 0.81 -0.6 209,692 - RR42_RS00865 0.81 -0.7 209,692 - RR42_RS00865 0.81 +1.5 209,693 + RR42_RS00865 0.82 +0.7 209,693 + RR42_RS00865 0.82 -3.0 209,693 + RR42_RS00865 0.82 +1.2 209,693 + RR42_RS00865 0.82 -0.2 209,694 - RR42_RS00865 0.82 +0.2 209,694 - RR42_RS00865 0.82 -1.8 209,694 - RR42_RS00865 0.82 -0.2 209,695 + RR42_RS00865 0.82 +0.7 209,695 + RR42_RS00865 0.82 +0.9 209,695 + RR42_RS00865 0.82 -1.2 209,695 + RR42_RS00865 0.82 -0.7 209,696 - RR42_RS00865 0.82 +0.1 209,696 - RR42_RS00865 0.82 -0.6 209,696 - RR42_RS00865 0.82 -0.6 209,764 - -1.2 209,801 + -0.6 209,802 - -0.1 209,802 - +0.2 209,802 - +0.1 209,901 + -0.2 209,901 + +0.6 209,901 + -0.3 209,901 + +0.1 209,901 + +0.5 209,902 - -1.0 209,902 - -1.1 209,902 - +1.3 209,902 - +0.9 209,902 - -0.8 209,902 - +0.1 209,902 - -0.6 211,155 + +0.2 211,184 - +0.4 211,240 + +0.7 211,381 + +0.1 211,381 + -0.1 211,426 + RR42_RS00875 0.12 -0.9 211,426 + RR42_RS00875 0.12 +0.2 211,427 - RR42_RS00875 0.12 -0.4 211,427 - RR42_RS00875 0.12 +0.3 211,441 - RR42_RS00875 0.13 -1.0 211,477 + RR42_RS00875 0.15 +0.0 211,478 - RR42_RS00875 0.15 -0.6 211,478 - RR42_RS00875 0.15 -0.5 211,564 + RR42_RS00875 0.20 -0.9 211,564 + RR42_RS00875 0.20 -0.3 211,565 - RR42_RS00875 0.20 -1.1 211,565 - RR42_RS00875 0.20 -1.4 211,612 + RR42_RS00875 0.22 -1.1 211,612 + RR42_RS00875 0.22 +0.2 211,612 + RR42_RS00875 0.22 +0.8 211,613 - RR42_RS00875 0.22 -1.1 211,658 - RR42_RS00875 0.25 +0.6 211,661 - RR42_RS00875 0.25 -0.4 211,711 - RR42_RS00875 0.28 -0.8 211,752 + RR42_RS00875 0.30 +0.3 211,759 + RR42_RS00875 0.30 +1.1 211,760 - RR42_RS00875 0.30 -0.4 211,760 - RR42_RS00875 0.30 -0.4 212,038 + RR42_RS00875 0.45 +0.5 212,038 + RR42_RS00875 0.45 +0.7 212,039 - RR42_RS00875 0.45 +1.4 212,039 - RR42_RS00875 0.45 -0.6 212,039 - RR42_RS00875 0.45 -0.7
Or see this region's nucleotide sequence