Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00640 and RR42_RS00645 overlap by 4 nucleotides RR42_RS00645 and RR42_RS00650 are separated by 95 nucleotides RR42_RS00650 and RR42_RS00655 are separated by 125 nucleotides
RR42_RS00640: RR42_RS00640 - allophanate hydrolase, at 166,582 to 167,232
_RS00640
RR42_RS00645: RR42_RS00645 - allophanate hydrolase, at 167,229 to 168,251
_RS00645
RR42_RS00650: RR42_RS00650 - hypothetical protein, at 168,347 to 169,087
_RS00650
RR42_RS00655: RR42_RS00655 - membrane protein, at 169,213 to 169,950
_RS00655
Position (kb)
167
168
169 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 166.397 kb on + strand at 166.411 kb on + strand at 166.412 kb on - strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.424 kb on + strand at 166.449 kb on + strand at 166.467 kb on - strand at 166.528 kb on + strand at 166.529 kb on - strand at 166.529 kb on - strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.546 kb on + strand at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.653 kb on - strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.658 kb on + strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.659 kb on - strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.668 kb on + strand, within RR42_RS00640 at 166.669 kb on - strand, within RR42_RS00640 at 166.951 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.960 kb on + strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.961 kb on - strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.990 kb on + strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.991 kb on - strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.992 kb on + strand, within RR42_RS00640 at 166.993 kb on - strand, within RR42_RS00640 at 167.095 kb on + strand, within RR42_RS00640 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.562 kb on + strand, within RR42_RS00645 at 167.563 kb on - strand, within RR42_RS00645 at 168.157 kb on - strand at 168.282 kb on + strand at 168.282 kb on + strand at 168.282 kb on + strand at 168.282 kb on + strand at 168.282 kb on + strand at 168.283 kb on - strand at 168.581 kb on + strand, within RR42_RS00650 at 168.581 kb on + strand, within RR42_RS00650 at 168.582 kb on - strand, within RR42_RS00650 at 168.596 kb on + strand, within RR42_RS00650 at 168.597 kb on - strand, within RR42_RS00650 at 168.597 kb on - strand, within RR42_RS00650 at 168.597 kb on - strand, within RR42_RS00650 at 168.675 kb on - strand, within RR42_RS00650 at 168.839 kb on + strand, within RR42_RS00650 at 168.839 kb on + strand, within RR42_RS00650 at 168.840 kb on - strand, within RR42_RS00650 at 168.840 kb on - strand, within RR42_RS00650 at 168.840 kb on - strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.965 kb on + strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.966 kb on - strand, within RR42_RS00650 at 168.969 kb on + strand, within RR42_RS00650 at 169.103 kb on + strand at 169.104 kb on - strand at 169.104 kb on - strand at 169.199 kb on + strand at 169.199 kb on + strand at 169.200 kb on - strand at 169.200 kb on - strand at 169.241 kb on + strand at 169.241 kb on + strand at 169.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 166,397 + -1.2 166,411 + +0.7 166,412 - +0.4 166,424 + -0.4 166,424 + -0.1 166,424 + -0.4 166,424 + +0.8 166,449 + -2.6 166,467 - +0.3 166,528 + -2.4 166,529 - +1.5 166,529 - -0.1 166,546 + -0.9 166,546 + -0.4 166,546 + +0.3 166,546 + +0.5 166,546 + +0.2 166,653 - RR42_RS00640 0.11 -0.0 166,653 - RR42_RS00640 0.11 -1.0 166,653 - RR42_RS00640 0.11 +0.2 166,658 + RR42_RS00640 0.12 -0.0 166,658 + RR42_RS00640 0.12 +0.3 166,658 + RR42_RS00640 0.12 -1.4 166,659 - RR42_RS00640 0.12 -0.8 166,659 - RR42_RS00640 0.12 +1.1 166,659 - RR42_RS00640 0.12 +0.4 166,659 - RR42_RS00640 0.12 +0.5 166,659 - RR42_RS00640 0.12 -0.0 166,659 - RR42_RS00640 0.12 +0.7 166,668 + RR42_RS00640 0.13 +1.4 166,668 + RR42_RS00640 0.13 +0.1 166,668 + RR42_RS00640 0.13 -1.1 166,669 - RR42_RS00640 0.13 +1.3 166,951 + RR42_RS00640 0.57 -1.0 166,960 + RR42_RS00640 0.58 +0.5 166,960 + RR42_RS00640 0.58 +0.3 166,961 - RR42_RS00640 0.58 +0.2 166,961 - RR42_RS00640 0.58 +0.5 166,990 + RR42_RS00640 0.63 +0.1 166,990 + RR42_RS00640 0.63 -0.4 166,990 + RR42_RS00640 0.63 -0.3 166,990 + RR42_RS00640 0.63 +0.6 166,991 - RR42_RS00640 0.63 +1.1 166,991 - RR42_RS00640 0.63 -0.1 166,991 - RR42_RS00640 0.63 +0.3 166,992 + RR42_RS00640 0.63 +0.0 166,992 + RR42_RS00640 0.63 -0.5 166,993 - RR42_RS00640 0.63 -0.2 167,095 + RR42_RS00640 0.79 -0.0 167,562 + RR42_RS00645 0.33 -1.9 167,562 + RR42_RS00645 0.33 -0.5 167,562 + RR42_RS00645 0.33 +0.1 167,562 + RR42_RS00645 0.33 -0.6 167,563 - RR42_RS00645 0.33 -0.7 168,157 - +0.4 168,282 + -0.5 168,282 + -0.2 168,282 + -0.4 168,282 + +1.0 168,282 + -2.6 168,283 - -0.6 168,581 + RR42_RS00650 0.32 -0.1 168,581 + RR42_RS00650 0.32 +0.5 168,582 - RR42_RS00650 0.32 -0.0 168,596 + RR42_RS00650 0.34 +0.8 168,597 - RR42_RS00650 0.34 +0.1 168,597 - RR42_RS00650 0.34 -0.7 168,597 - RR42_RS00650 0.34 +0.2 168,675 - RR42_RS00650 0.44 -1.2 168,839 + RR42_RS00650 0.66 -0.0 168,839 + RR42_RS00650 0.66 +0.7 168,840 - RR42_RS00650 0.67 +0.1 168,840 - RR42_RS00650 0.67 -1.9 168,840 - RR42_RS00650 0.67 -1.0 168,965 + RR42_RS00650 0.83 -1.5 168,965 + RR42_RS00650 0.83 -0.1 168,965 + RR42_RS00650 0.83 +0.5 168,965 + RR42_RS00650 0.83 -0.4 168,965 + RR42_RS00650 0.83 +0.1 168,965 + RR42_RS00650 0.83 -0.1 168,966 - RR42_RS00650 0.84 +0.3 168,966 - RR42_RS00650 0.84 -0.7 168,966 - RR42_RS00650 0.84 -0.6 168,966 - RR42_RS00650 0.84 +0.8 168,966 - RR42_RS00650 0.84 -0.2 168,966 - RR42_RS00650 0.84 +0.4 168,966 - RR42_RS00650 0.84 +0.4 168,966 - RR42_RS00650 0.84 -1.0 168,966 - RR42_RS00650 0.84 -0.5 168,969 + RR42_RS00650 0.84 +0.6 169,103 + -0.0 169,104 - +0.4 169,104 - -0.4 169,199 + +0.2 169,199 + +2.1 169,200 - +0.5 169,200 - -0.1 169,241 + +0.2 169,241 + -0.4 169,242 - -1.3
Or see this region's nucleotide sequence